Zeiss LSM 880, Operating Manual

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CHAPTER 5 - MULTIPHOTON LASER SCANNING …
LSM 880 Non Descanned Detection (NDD) ZEISS
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GaAsP NDD 2 channels for Axio Examiner
This detector module is located in a special objective holder. It replaces a standard objective holder on the
Axio Examiner. It holds one objective. Standard objectives need to be used together with the optical
adapter provided.
For attaching the holder to the microscope, see the safety instructions. Make sure the detector is then
connected to the realtime electronics and the power supply as described in section 2.3of this chapter.
To indicate the detector to the software use the configuration tool in the following way:
Open the tab for the Microscope and choose the tab Objectives. In the top most drop-down list choose
the 1 position Objective nosepiece manual (Fig. 15).
Fig. 15 Configuration tool with Microscope and Objectives tab active for choosing the objective
nosepiece representing also the GaAsP NDD 2 channels
In addition indicate the presence of the GaAsP
NDD 2 channels in the Scan Head/ External
Detectors tab (Fig. 16).
The GaAsP NDD 2 channels provides two highly
sensitive GaAsP detectors which are located inside
the objective holder.
It is a very light sensitive detector which is
protected against too much light by automatic
down-regulation of the voltage or even the shut-
down of the voltage. An additional hardware
shutter closes if the detector is not used for image
acquisition. See further instructions on handling
the detector in the Safety instructions.
Fig. 16 Configuration tool with External
Detectors tab active for activating
the GaAsP NDD 2 channels