lead with these objectives to decreased image quality, drying of systems of high aperture reacts very sensitively! Uncovered preparations
require the observation with whether jektiven, which - with apertures >0,25 - is counted for this purpose. Marking e.g.: GF-PA
50x/0,80
∞
/0 (see to it Bestelliste). One observes at present inzunehmendem measure also uncovered biomedical preparations (large
series of celldeleted or thin cut). Absolutely consider:
Most uncovered (colored) would celldelete oderDünnschnitte lead when observation with Trockensystemennur to
good image quality if they are covered with lacquer. The lacquer prevents the “Verschwärzlichung " of the
object colors and superelevated outline contrast, those as consequence to large refractive index differences
between object
and develop for surrounding air. Commercial cover glass lacquer with brush or as spray as thinly as possible (!) up
carry.
With cover glass lacquer covered preparations are to be observed with drying systems, which are without coverglass-counted
for preparations, if the objective apertures 0.25 over walk!
(4)
adjustable attack for condensor lenses
At the admission for the condensor lens A TRAILEr a set screw (fig. 3/31) is, with which an attack can be specified for those-highest
position of the condensor lenses. To the operation:
•
Preparation on slides with “standard thickness” select
(e.g. Thick ones of the slide l, to 0 mm) and on microscope table for observation furnish.
Set screw (fig. 3/31) on approximately 10 mm of length out screw.
Annunciator screen picture focus and center
Set screw so far screw in, until clear against stood becomes perceptible. The condensor lens must sharp-illustrate the annunciator
screen after lowering and again raising to attack.
In the case of observation of all following preparations maintain the ge fundene attack position of the condensor lens (or if
necessary again with condensor lens impulse start), to correct without those sharpness of the annunciator screen picture.
Operating advantage when using slides, of them
Thick one not too much of the selected “standard thickness”
deviates!
If the full cut width of the condensor lenses must be used (e.g. for accurate lighting after Köhler with thicker slides), set screw on
approximately 10 mm of length out to screw. The attack is waived thereby. Also in this condition it is guaranteed that the achromatic
aplanatische of condensor lens 0.9 beyond the table surface and can dig the preparation.
(5) the Bertrandlinse/work with the aperture diaphragm
With the help of the Bertrandlinse it is possible to make the withdrawal pupil of the objective in image field visible as well as in the
proximity of the pupil-lying pictures of some optical elements and these elements controlled serves, e.g. Aperture diaphragm and
contrast modulators (lightinglateral ring screens and picture-lateral phase rings for phase contrast).
Operation:
Lighting after Köhler stop.
