Axioskop 2
plus
OPERATION
Axioskop 2
mot plus
Illumination and contrasting techniques
Carl Zeiss
B 40-075 e 02/01
3-25
•
Swing in 10x objective (yellow ring, see p. 1-12)
in the nosepiece (3-17/
6
) and focus on the
specimen via drive (3-17/
).
•
Close luminous-field diaphragm (3-17/
) until it
becomes visible (even if not in focus) in the field
of view (3-17/
).
•
Use drive for height adjustment (3-18/
2
) to
lower condenser until the edge of the
luminous-field diaphragm appears in focus
).
•
Use both centering screws (3-18/
3
) of the
universal condenser to center the luminous-field
diaphragm (3-17/
C
) and then open the
diaphragm until its edge just disappears from
the field of view (3-17/
D
).
•
For aperture diaphragm setting (contrast),
remove one eyepiece from the tube and look
into the tube with your naked eye. Use sliding
knob (3-17/
) to set the aperture diaphragm to
approx. 2/3
...
4/5 of the diameter of the
E
). In most
applications, this aperture diaphragm setting
provides optimum contrast at almost ideal
resolution, and is therefore the best
compromise for the human eye.
•
Insert eyepiece in the tube again.
☞
Since field size and objective aperture change after every objective change, the setting of the
luminous-field diaphragm and the aperture diaphragm must be repeated to obtain optimum
results.
For < 10x objectives, the front lens of the condenser must be folded out and the aperture
diaphragm fully opened. In the case of such large fields, the luminous-field diaphragm can also
be used for better contrasting by reducing its opening until it becomes visible in the field of
view.
1
0
2
0
30
40
50
6
0
7
0
80
90
100
Fig. 3-17
Microscope settings on the
Axioskop 2
plus
in transmitted-light
brightfield
