OPERATION
Axioskop
2
plus
Carl Zeiss
Illumination and contrasting techniques
Axioskop 2
mot plus
3-26
B 40-075 e 02/01
(4) Setting the height stop on the
condenser carrier
•
Loosen fixation screw of height stop (3-18/
1
)
using SW 3 ball-headed screwdriver.
•
Use focusing drive to focus on the specimen.
•
Close luminous-field diaphragm and image it in
focus via the height adjustment control (3-18/
2
)
of the universal condenser.
•
Carefully lift the condenser slightly without
touching the specimen.
•
Tighten fixation screw (3-18/
1
) of the height
adjustment.
(5)
Transmitted-light brightfield configuration of the Axioskop 2
mot plus
Each Axioskop 2
mot plus
configuration permits the performance of the transmitted-light brightfield
technique.
(6)
Setting the Axioskop 2
mot plus
for transmitted-light brightfield according to KÖHLER
−
The Axioskop 2
mot plus
has been set up as described in chapter 2.
−
Programming of the stage movement in Z, which covers the matching of focus positions and focus
speeds of the used objectives, has been finished.
−
The instrument is switched on and is in the automatic mode.
Illumination intensity, condenser settings (position of the front lens, position of the turret disk and the
opening of the aperture diaphragm) and the status "transmitted light switched on or off" are stored by
pressing the Set key.
•
Setting of KÖHLER illumination to be performed in the same way as described in section 3.3.1 (3) for
the manual Axioskop 2
plus
. Unlike this procedure, however, the luminous-field diaphragm must be
fully opened after the correct setting of the condenser. Shortly press the Set key on the instrument
rear after the last setting step to store the settings. A short beep is heard when the Set key is released
to indicate that storage has been performed.
•
The above diaphragm settings must be repeated for all objectives, since field size and objective
aperture have different values.
☞
For < 10x objectives, the front lens of the universal condenser must be folded out and the
aperture diaphragm fully opened.
Fig. 3-18
Setting the height stop on the
condenser carrier
