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WF-Zeiss_EN_V3 24/01/2017
Start Zen / Observation with the oculars
1.
Start the « ZEN » software (Icon on the desktop).
2.
The stage calibration is necessary only if you use
multiwell plates or if you want to retrieve positions
from a past experiment
3.
Select the tab « Locate ».
4.
« HAL» allows you to observe your sample in brightfield (transmission).
If you want to observe in phase contrast or DIC, first
select « HAL » then select « PH » or « DIC » on the TFT
screen
5.
« DAPI » to observe in blue.
6.
« CFP » to observe in cyan.
7.
« GFP » to observe in green.
8.
« DsRed » to observe in orange-red.
9.
« Cy5 » to observe in far red.
10.
« Light off » close the shutter for the transmission
and the fluorescent light.
For transmitted light acquisitions, phase contrast or DIC, it is important to make adjustments
on the microscope (set the Khöller illumination…)
Refer to the panels in the room of the microscope.
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