8
•
Automatic movements can be interrupted by pressing the STOP button. Prevent
the objective from colliding with the specimen or transillumination base. The end
positions for the motorized range of movement should be set when appropriate
•
When the motorized focusing drive is lowered, there is the risk of pinching
fingers in the working area. Do not reach into the working area or beneath the motorized
focusing drive during lowering
•
The diaphragm slide should initially be at the rear stop in order to achieve a large
homogeneous luminous field. Pull it slowly to the front while observing the microscopy
image to achieve the desired effect
•
The transmitted light can be completely covered with the diaphragm slide (front
stop) to achieve a dark background, e.g. for fluorescence illumination
•
Slight careful deflection of the joystick results in fine focusing, while full
deflection produces faster focusing movements as a course screw would produce
•
If you personalized some ZEN 2 pre-setting, reset the software for the default
definitions. Do not save any alteration to prior experiment sets
•
Make sure you have the correct tube opening regarding the intended light path
(eyepieces or camera)
•
Save all data into your own storage device or cloud as we routinely clean the
computer’s data
•
Do not forget to register your utilization in the logbook
•
A free software license is available for ZEN lite at ZEISS website. Install and use
such version in your own personal computer for downstream image analysis (see
Downstream Image Processing and Analysis)
•
Contact the responsible people in case of any doubt
Potential Techniques
•
Large-Field Observations
•
Large-Field
in vivo
Timelapses
•
Biofilm Observation
