Cancer Institute
Microscopy Core Facility
V1.0
Fig 7 – C tab of Multidimensional Acquisition
h)
Ensure the correct condenser filter is
selected for application – Fig 8
H – Brightfield
2 – Phase contrast in x20 objective
3 – Phase Contrast in x40 and x60
Objectives
I,II and III are for DIC applications which are
not currently installed, treat as brightfield
1 is intended for phase contrast using an x10
objective which is not currently installed
i)
It is now possible to select the optimal
settings for each channel by clicking
on each channel separately and
selecting measure in the exposure
box – This will automatically select an
optimal exposure time, if you desire a
known exposure time to generate
comparable images select the desired
exposure
time.
The
Exposure
Measurement window will open.
Adjust exposure here or click ok
Figure 8 – Condenser Controls.
Condenser
aperture
slider
Condenser
filter wheel
