Zeiss ApoTome.2, Operation Manual

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INSTRUMENT DESCRIPTION
Carl Zeiss Suitability of objectives for the use of ApoTome.2 ApoTome.2
20 423667-7144-001 05/2012
Order No. Name Excitation / Color splitter / Emission Fluorophores (examples)
488026-9901-000 FS 26
BP 575-625
FT 645
BP 660-710
mPlum, Nile Red
489038-9901-000 FS 38 HE
BP 470/40
FT 495
BP 525/50
Alexa 430, Alexa 488, FITC
489043-9901-000 FS 43 HE
BP 550/25 (HE)
FT 570 (HE)
BP 605/70 (HE)
Alexa 546, Alexa 555, Cy3, DiI
489046-9901-000 FS 46 HE
BP 500/25
FT 515
BP 535/30
Alexa 488, FITC, eGFP, eYFP
489047-9901-000 FS 47 HE
BP 436/25
FT 455 HE
BP 480/40 CFP
488049-9901-000 FS 49
G 365
FT 395
BP 445/50
DAPI
488050-9901-000 FS 50
BP 640/30
FT 660
BP 690/50
Alexa 647, Alexa 660, Cy5
489059-9901-000
FS 59 HE
(Colibri)
BP 445/25 (HE)
BP 510/15 (HE)
DFT 460 + 520 (HE)
DBP 480/22 + LP 530 (HE)
Alexa 433, Alexa 488, CFP, FITC, eYFP
489060-9901-000
FS 60
(Colibri)
BP 445/25 (HE)
BP 510/15 (HE)
BP 588/27 (HE)
TFT 460 + 520 + 605 (HE)
DBP 480/22 + 546/32 + LP 615
Álexa 430, Alexa 568, Alexa 594
489061-9901-000
FS 61
(Colibri)
BP 474/28 (HE)
BP 585/35 (HE)
DFT 495 + 605 (HE)
DBP 527/54 + 645/60 (HE)
CFP, YFP, mRFP
489062-9901-000
FS 62
(Colibri)
BP 370/40 (HE)
BP 474/28 (HE)
BP 585/35 (HE)
TFT 395 + 495 + 610 (HE)
TBP 425 + 527 + LP615 (HE)
DAPI, CFP, YFP