WPI PIMT201, Instruction Manual

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20 World Precision Instruments
6. Slide the phase aperture fame into position with the 10X/20X condenser annulus
in the condenser light path. ( Fig. 63 )
NOTE : If you are using a 40X phase contrast objective (not included), use the 40X
condenser annulus on the phase aperture frame.
Fig. 63—(Left) Plate position by phase
Fig. 64—(Right)Aperture disc seen through the telescope
7. You will see an overlap of the condenser annulus and phase ring patterns
through the phase telescope ( Fig. 64 ). To optimize the phase contrast image, the
condenser annulus must be concentrically aligned with the phase ring. The two
rings can be seen through the telescope. See Fig. 60 , page 18.
8. To adjust the condenser annulus, insert the large hex wrench into the adjustment
holes on either side of the phase aperture frame as shown in Fig. 65 and Fig. 66 .
Tightening the hex screw in the left side moves the plate towards the upper right
corner. Loosening it moves the plate towards the lower left corner. The opposite
adjustments can be made with the hex wrench in the right opening. See Fig. 67
for a schematic representation of the appearance of the phase contrast masks
both in and out of alignment.
Fig. 65—Insert hex wrench in the right opening
Fig. 66—Make adjustments on the left side