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10 

World Precision Instruments

Manual mode

Online mode

Offline mode

Master manual mode

Download mode

The modes can also be viewed and selected with the following icons located on the 

toolbar. From left to right, these icons represent Manual mode, Online mode, Offline 

mode, Master Manual mode and Download mode.

Computer Perfusion Control Modes

Online Mode–

In this mode, perfusion is controlled by the computer software in real 

time. 

Run

Pause

 and 

Stop

 can be controlled from the Operation menu, the toolbar 

icons 

, or with the function keys (F8, F9 and F10 as shown on the Operation 

menu). 

Data Download Mode–

The experimental procedure created with the software is 

downloaded into the RAM of the 

MPS-4

 control box when you press the  button or 

select Download from the Operation menu.  The Online LED on the 

MPS-4

 control 

box blinks while the program is being transferred.

MPS-4 Controller Operation

–The left section of the 

MPS-4

 front panel contains the 

Start

Reset

 and 

Mode

 buttons, and three functional indicator LEDs:

• 

Start

 button–The button runs the perfusion process. In the Online mode, it acts 

as the 

 button and runs the experiment controlled by the perfusion software. 

In Offline mode, the 

Start

 button runs the preset perfusion parameters saved to 

the RAM of the control box from the computer.

• 

Reset

 button is the zero button for the micro-controller. It stops any currently 

running experiment, turns all channels off and reset the mode to Manual.

• 

Mode

 button toggle the perfusion mode between Manual, Online, Offline and 

Master Manual. Once a mode is selected, the corresponding LED illuminates. In  
Master Manual mode, the Manual LED blinks continuously. The operation of each 
mode is described in the following sections.

Run Perfusion

Normal Mode–

In this mode, the 8 channels are independently controlled by pressing 

the channel buttons. When a channel is on, its LED lights up.

Master Channel Mode

–This is just like the normal Manual mode except that only 

one channel can be on at a time. If all other channels are turned off, channel 8 (the 

master channel) automatically turns on. When this mode is first selected, it is in the 

inactive state in which all channels are turned off. Press the channel 8 button to toggle 

between the active and inactive state.

Summary of Contents for MPS-4

Page 1: ...WORLD PRECISION INSTRUMENTS MPS 4 Multichannel Perfusion System Serial No _____________________ www wpiinc com INSTRUCTION MANUAL 011720...

Page 2: ......

Page 3: ...Installation 6 Installation 6 Startup 6 Creating a New Perfusion Experiment 6 Set the Experiment Time 7 Preset Each Channel s Perfusion Time 7 Saving Your Experimental Parameters 9 Mode Selection 9 Co...

Page 4: ...ii World Precision Instruments...

Page 5: ...nfluence of metal ions in the moving iron of the solenoid valve on the experimental results Unlike other perfusion systems on the market which often compromise performance to fit every possible applic...

Page 6: ...me is less than 100 nL The injection molded manifold is also designed as an economical disposable item eliminating problems of cross contamination from other experiments Notes and Warnings NOTE Before...

Page 7: ...0mL Syringes 10 3 way Stopcock 10 Luer fitting with barb for 1 16 ID tubing 5 Color Coded Polyurethane Tubing Ribbon 1 manifold Holding Rod 1 manifold with 100 m ID tip 1 manifold with 250 m ID tip 1...

Page 8: ...of this manual INSTRUMENT DESCRIPTION Hardware Installation 1 Set the MPS 4 perfusion stand on a stable platform 2 Insert the stainless steel post into the base and tighten the screw 3 Loosen the scre...

Page 9: ...TING INSTRUCTIONS Fig 3 The front panel of the MPS 4 has the operational controls The left section of the MPS 4 front panel contains the Start Reset and Mode buttons and three functional indicator LED...

Page 10: ...install an unsigned driver on Windows 8 on page 19 Appendix D Install the MPS 4 software on Windows 10 on page 22 Startup The perfusion software can be found in the Start menu of Windows under C Prog...

Page 11: ...with the keyboard Move to the next field by filling in both digits of the field or by pressing the spacebar Click Apply when all fields are set to the desired values The perfusion time shown at the ri...

Page 12: ...el click Apply to verify the changes Otherwise the new values will be lost when a different channel is chosen or the Setup window closes 5 You can also set the perfusion time by holding the left mouse...

Page 13: ...der to change the operation mode or choose the serial port choose Mode Setup from the Operation menu press the F3 function key or click the button in the Tool Box The Mode Setup window pops up Click o...

Page 14: ...on The button runs the perfusion process In the Online mode it acts as the button and runs the experiment controlled by the perfusion software In Offline mode the Start button runs the preset perfusio...

Page 15: ...de Press any of the Channel keys if the experiment has to be manually halted This will exit out of TTL Control mode and prevent it from going back into that mode until Reset is pressed or the instrume...

Page 16: ...t the tip of the micro perfusion head is close to the bottom of the dish The access angle is about 35 45 5 Pull a 1 m micropipette Fill the pipette with 150mM NaCl solution to make it a microelectrode...

Page 17: ...lush it CAUTION Unfiltered water could clog the manifold and permanently damage it The manifold is made of PMMA material It can only be washed with water Any organic solvent even alcohol can permanent...

Page 18: ...210x170 mm APPENDIX A DETERMINING FLOW RATE Theoretical Calculation The relationship of flow rate to the height of the liquid column and inner diameter of the capillary tubing can be accurately predic...

Page 19: ...driver Please follow those steps below 1 Since you need to restart the computer multiple times during the operation you need to save your unfinished work and exit all applications before the disable d...

Page 20: ...uments 3 Click the option Update security and a new window appears Fig 14 Select the Recovery tab 4 After selecting the Recovery tab the Advanced startup area appears on the right side of the window F...

Page 21: ...restart 6 After restart the Option screen displays Fig 16 Click Troubleshoot 7 After clicking the option Troubleshoot the Troubleshoot screen appears Fig 17 Click Advanced Options 8 Click the option...

Page 22: ...again The following menu appears on the screen after the restart Fig 20 Press 7 on your keyboard 11 You can press 7 on your keyboard to choose the Disable driver signing enforcement The computer will...

Page 23: ...low the steps shown below to start Windows 8 in Disable Driver Signature Enforcement mode 1 Move the cursor over the top or bottom right corner or the screen and it will show extra options on the righ...

Page 24: ...n the left hand side and then click Restart now button on the right Fig 23 Select the General tab and click Restart now 4 After a restart click Troubleshoot Fig 24 Select the Troubleshoot option 5 Cli...

Page 25: ...Fig 27 Press Restart 8 Choose the Disable Driver Signature Enforcement and hit the Enter key to start Windows Fig 28 Select the Disable Driver Signature Enforcement option and press Enter 9 You shoul...

Page 26: ...e click on it to begin the installation 3 Using the provided USB cable connect the USB port of host computer to the USB port square of the MPS 4 controller and then turn on the power of the MPS 4 cont...

Page 27: ...on your computer depending on what is installed on your computer When the device manager is opened the Other devices Unknown device with yellow background exclamation mark will appear in the director...

Page 28: ...nts 7 Click the Update Driver button The following window appears Fig 33 Select the option to Browse my computer 8 Select and click Browse my computer for driver software A similar window appears Fig...

Page 29: ...from running MPS 4 19 07 exe during the installation For example on the this computer the MPS 4 program was specified to be installed in the following directory location C Program Files x86 MPS 4 Dri...

Page 30: ...ver software was installed from running MPS 4 19 07 exe in step 2 Click the Next in the lower right corner and the following window appears Fig 38 Click the Install option 13 Select and click the Inst...

Page 31: ...ontrollers section and confirm a successful installation of the driver software NOTE After the installation of the MPS 4 driver software a system reboot automatically resumes the state of the driver s...

Page 32: ...28 World Precision Instruments...

Page 33: ...MPS 4 World Precision Instruments 29...

Page 34: ...30 World Precision Instruments...

Page 35: ...ecial or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or ind...

Page 36: ...n Germany Tel 49 0 6031 67708 0 Fax 49 0 6031 67708 80 E mail wpide wpi europe com China Hong Kong WPI Shanghai Trading Co Ltd Rm 27A Liangfeng Building No 8 Dongfang Road Pudong Free Trade Zone Shang...

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