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EVOM3

World Precision Instruments

be reproduced or translated into any language, in any form, without prior written permission of World

Precision Instruments, Inc.

CONTENTS

QUICK START ................................................................................................................................... 1

Resistance ................................................................................................................................... 1

Voltage ......................................................................................................................................... 1

TEER Calculations ...................................................................................................................... 2

ABOUT THIS MANUAL ................................................................................................................... 3
INTRODUCTION .............................................................................................................................. 3

EVOM3 Features ....................................................................................................................... 4

EVOM3 Benefits......................................................................................................................... 4

STX2-PLUS Features ................................................................................................................. 5

STX2-PLUS Benefits .................................................................................................................. 5

Notes and Warnings ................................................................................................................. 5

Tips and Advice .......................................................................................................................... 6

PARTS LIST ........................................................................................................................................ 7

Unpacking ................................................................................................................................... 8

INSTRUMENT DESCRIPTION ........................................................................................................ 9

EVOM3 Meter ............................................................................................................................. 9

STX2-PLUS Electrodes.............................................................................................................. 9

Theory of Operation ...............................................................................................................10

Main Display .............................................................................................................................10

Setup Page ................................................................................................................................11

Plate Selection Page and Plate Preview .............................................................................11

Mode Units ...............................................................................................................................12

Blank Handling Page ..............................................................................................................13

Setting the Date and Time ....................................................................................................13

Probe Null .................................................................................................................................14

Storing Data ..............................................................................................................................15

Viewing Data in Microsoft Excel or Other Spreadsheet Program ...............................16

USB Testing ..............................................................................................................................17

SYSTEM MESSAGES ......................................................................................................................18

Last Cell Warning ....................................................................................................................18

Power Level Messages ...........................................................................................................18

Reloading a Backup File after Power Failure ....................................................................20

OPERATIONS ..................................................................................................................................21

Instrument Diagnostics and Calibration............................................................................21

Making Resistance Measurements .....................................................................................22

Making Voltage Measurements ...........................................................................................24

Summary of Contents for EVOM3

Page 1: ...WORLD PRECISION INSTRUMENTS EVOM3 Epithelial Volt Ohm Meter Serial No _____________________ www wpiinc com INSTRUCTION MANUAL 121520...

Page 2: ......

Page 3: ...ts 5 Notes and Warnings 5 Tips and Advice 6 PARTS LIST 7 Unpacking 8 INSTRUMENT DESCRIPTION 9 EVOM3 Meter 9 STX2 PLUS Electrodes 9 Theory of Operation 10 Main Display 10 Setup Page 11 Plate Selection...

Page 4: ...FILE FORMATS 36 Resistance CSV 36 Voltage csv 36 APPENDIX C CONTINUOUS RECORDING VIA USB PYTHON ON A PC 36 Running the External Data Connection Output Program PC Mac Linux 37 Sample CSV recording 39...

Page 5: ...Store button on the screen to store and advance to the next weel Tap the Next Well area on the right side of the screen to advance in increments of one well without taking a measurement Repeat the sam...

Page 6: ...he next well Repeat the sampling process 5 Once the last well has been recorded the file storing selection notification appears Press Store A system message will report if the USB thumb drive is usabl...

Page 7: ...s on either PC or Mac The processor controlled instrument is more stable and accurate Included is a footswitch for quick hands free recording to the USB drive The touch screen interface is easy to use...

Page 8: ...or 10 A Resistance auto ranging from 1 to 100 000 or with three fixed current ranges Reliable low current low voltage design prevents metal ion transport Fast resistance stabilization on low levels un...

Page 9: ...Li ion battery will last 8 hours on its own The charging time is 5 5 hours with the unit powered off The EVOM3 charger should not be plugged in all the time The chager may be used to power the EVOM3...

Page 10: ...e levels that are unexpectedly lower or higher can be attributed to a number of things besides an incompletely grown membrane Any of these items can change the resistance reading NOTE Many of these co...

Page 11: ...helial Volt Ohm Meter 1 STX2 Plus Electrode set 1 300749 USB drive 32 GB For storage and this contains a Python 3 8 program for continuous digital monitoring of a target insert See page 36 1 503535 US...

Page 12: ...e if any parts are missing at 941 371 1003 or customerservice wpiinc com Returns Do not return any goods to WPI without obtaining prior approval RMA required and instructions from WPI s Returns Depart...

Page 13: ...Precision Instruments 9 INSTRUMENT DESCRIPTION EVOM3 Meter Fig 3 USB port Electrode connection Fig 4 Foot switch Future expansion reset power connection power switch STX2 PLUS Electrodes Fig 5 STX2 PL...

Page 14: ...the other two electrodes and computes resistance using Ohms law The polarity of the current changes from positive current to negative current 12 5 times per second This polarity change avoids leaving...

Page 15: ...4 8 hours to remove the charge See also Probe Null on page 14 Date Time Select the Date and Time formats for your data files Calibrate Calibrate the electrode This uses a 10K 0 1 resistor See Instrum...

Page 16: ...well plate preview Fig 13 96 well plate preview Press scroll to see the additional wells NOTE An out of resistance range will display 5 dashes on the screen Adjust the range to correct the error A sav...

Page 17: ...ding 2 Insert the probe into an empty well filled with media Press Reading to make a measurement 3 Select the Blank Enable radio button to enable the offset 4 Press Return The resistance offset shows...

Page 18: ...on the Setup page to access the NULL Probe Process screen Fig 22 Left Null probe process Fig 23 Right The potential difference offset shows on the main screen under the reading 2 Place the electrodes...

Page 19: ...g 25 Press Clear Type the desire name Press Enter 4 If you prefer to use the Auto Index function press Store Prefix to enter a unique file name that will be appended with sequential numbers each time...

Page 20: ...ge offset Units ohms or kilo ohms Recording direction 1 4 See Plate Selection Page and Plate Preview on page 11 Resistance scale 10K 50K 100K and the last used scale is shown Well data in the plate gr...

Page 21: ...the Test USB button to run the test or Return to abort the test The USB drive will be written to with 100 lines of data to a file named USBTEST txt and those lines will be read back If the drive is u...

Page 22: ...dication that the last well in the plate has been reached and the file needs to be stored Press Store to save using the current file name or the prefix file name Or press Cancel to change the file nam...

Page 23: ...le When the battery level falls to 0 the system begins a 60 second countdown before powering off Fig 32 When the battery power falls to 0 a warning displays briefly Fig 33 The 60 second countdown was...

Page 24: ...ed when the unit is plugged into a power source 2 Cycle power on the EVOM3 by turning it off and on again This will automatically load the backup file which was saved when the power failed The backup...

Page 25: ...Ohms and 10 000 Press Return 3 Verify that the blank handling is disabled Setup Blank Handling The screen should display 1000 1 If it does not select Setup push the Calibration button and wait for th...

Page 26: ...re your stored data files Fig 38 Store options screen To use a prefix and auto indexing activate the Auto Index radio button Then press Store Prefix to enter a file name Or to use a unique file name d...

Page 27: ...ouching the Store button on the screen Tap the Next Well area on the right side of the screen to advance to the next well Repeat the sampling process 5 Once the last well has been recorded the file st...

Page 28: ...e the Auto Index radio button Then press Store Prefix to enter a file name Or to use a unique file name deactivate the Auto Index radio button Then select File Name to enter a new name Fig 45 Keyboard...

Page 29: ...de of the screen to advance to the next well Repeat the sampling process 5 Once the last well has been recorded the file storing selection notification appears Fig 49 Warning that the last cell measur...

Page 30: ...buffer solution 4 Make your experimental measurements 5 Following electrode use soak the electrode tips in 70 ethanol for 10 minutes 6 Rinse with distilled water and let them air dry Store electrode...

Page 31: ...e steril ization methods for the electrodes include gamma irradiation and ethylene oxide gas ETO CAUTION Do NOT autoclave the electrode CAUTION Do NOT use ionized hydrogen peroxide H2 O2 CAUTION Do NO...

Page 32: ...operating properly a resistance test in KCl is used This test demonstrates electrode functionality 40 mM KCl 80 mM KCl 160 mM KCl 38 66 27 44 12 20 At the lower molarities 10 20 mM a DMEM coated elec...

Page 33: ...ar Snapwell cup 6 wells per plate EVOM CAL EVOM STX Calibration Solution TEER CL TEER Electrode Chloride Solution EndOhm chambers require EVOM3 cable 99916 Electrode Adapter WPI 3993 converts into fou...

Page 34: ...individually removable It can also be used for studying the tight junction changes induced by chemicals and other factors Concentric pairs of electrodes above and below the insert membrane results in...

Page 35: ...urs NOTE Make sure the EVOM3 is powered off when charging the battery Unit does not charge or charging LED not lit Insecure con nection at power input jack on meter Verify that the power connector is...

Page 36: ...witch in the OHMS position See Probe Null on page 14 for information on how to null the electrodes Dirty electrode Clean the electrode See Cleaning Maintaining the STX2 PLUS Electrodes on page 26 Cont...

Page 37: ...ormation of an insulative coating of foreign material that electrically blocks the current and voltage electrodes Remove the foreign material and retest See Testing the STX2 PLUS Electrodes on page 28...

Page 38: ...n the plate is changing Use a plate warmer In a 5 CO2 envi ronment a loss of CO2 causes the me dia pH to change and the resistance reading may in crease sharply A 5 CO2 environment can help in reducin...

Page 39: ...nzymatic cleaning See Cleaning Maintaining the STX2 PLUS Electrodes on page 26 SPECIFICATIONS This unit conforms to the following specifications Tissue Sampling Frequency 12 5 Hz Sample Averaging 20 s...

Page 40: ...of 20 mM KCl 25 mL 50 mL 10 mM KCl APPENDIX B FILE FORMATS Resistance CSV Date 03 12 2020 13 11 56 Well Plate 24 EVOM3 Version 1 0 MODE OHMS offset 999 8 Units OHMS Direction 1 Resistance Scale 50k Oh...

Page 41: ...ng the External Data Connection Output Program PC Mac Linux Windows 8 or above users WPI provides a USB keydrive also known as a thumb drive with all the software needed to run the External Data Outpu...

Page 42: ...do not have Python installed download and install Python for your operating system from www python org Be sure to download the version appropriate for your operating system The minimum supported Pyth...

Page 43: ...d The readings are continuous at 12 5 Hz always on The time entered is the averaged 20s s reading taken at that time interval Note 2 seconds is recommended 10 The default file name is WPI_EVOM3 csv Th...

Page 44: ...ed from inserts of different sizes For 6 well plates 24 mm inserts the STX2 PLUS can be used to take readings at the 12 o clock 3 o clock 6 o clok and 9 o clock positions to look for low resistance po...

Page 45: ...6G is higher The shape of the current electrodes allows a more uniform current density to flow across the membrane Fig 52 demonstrates how the STX electrode measures TEER and Fig 53 shows how the End...

Page 46: ...ng an EVOM3 with an STX2 PLUS to measure a blank insert the resistance value is typically between 60 to 180 depending on the specific brand of the insert and lower in EndOhms This is not background re...

Page 47: ...plying the meter readings by the effective surface area of the filter membrane The dimension is cm2 The resistance is inversely proportional to the surface area Thus the larger the membrane the lower...

Page 48: ...9916 adaptor is required or a new cable 2 When do researchers want to use the blank function Answer The blank function allows researchers to subtract out resistance measurement that is not caused by t...

Page 49: ...under sole responsibility that the product s WPI PN EVOM3 To which this declaration relates is are in conformity with the following standards or other normative documents Safety EN 61010 1 2010 A1 201...

Page 50: ...46 World Precision Instruments...

Page 51: ...ecial or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or ind...

Page 52: ...Tel 49 0 6031 67708 0 Fax 49 0 6031 67708 80 E mail wpide wpi europe com China Hong Kong WPI Shanghai Trading Co Ltd Rm 27A Liangfeng Building No 8 Dongfang Road Pudong New District Shanghai PR China...

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