Requirement:
Unless the system administrator has disabled the System
Secure function, you must be a registered user and provide your user name
and password, to start and log on to the ChromScope software.
4. Enter your user name and password, and then click Login, to start the
ChromScope software.
5. On the System Configuration Detector tab, click Lamp On.
Requirement:
Allow the lamp to warm for a minimum of 30 minutes before
performing an injection, to stabilize its optics.
6. Inspect the column's tubing connections to ensure that the column or unions
are connected, leaving no open flows.
7. Inspect the gradient pump's solvent bottles for mobile phase and wash flows,
to ensure the volumes are adequate for your methods.
Preparing the solvent pump
When the solvent lines are dry, including for initial startup, or when changing
solvents, prime the pump's solvent and seal-wash flow paths, to remove any air
bubbles in the flow paths between each solvent bottle and pump. To prime the 2545
QGM or 2545 BGM, double-click the solvent pump in the ChromScope system
panel, and then refer to priming instructions in the
2535/2545/2555 Quaternary
Gradient Module Operator's Guide
.
Preparing the sample injector
You must prepare the sample injector according to its type, manual or 2707 Auto-
sampler.
To prepare the manual injector:
1. With the column offline, flush the injector and sample loop with a suitable
solvent, to remove any trapped air or contaminants from the injection flow
path.
2. Prepare your sample for loading by inserting a syringe into the injector port.
To prepare the 2707 Autosampler:
1. Load samples, and ensure the wash-solvent bottle is full.
2. When the solvent line is dry, including for initial startup, or after changing
solvents or samples, on the System Configuration Autosampler tab, click
Needle Wash, to clean and purge any air bubbles from the syringe and buffer
tubing.
Tip:
For best results, repeat the needle wash routine 2 or 3 times.
Preparing the fraction bed and waste container
You must prepare the WFC III module to collect fractions and waste during a puri-
fication run.
August 8, 2014, 715003940 Rev. B
11
Start the system
