Solvent Considerations
97
C
Water
Use water only from a high-quality water purification system. If the water system does not
provide filtered water, filter it through a 0.45-µm membrane filter before use.
Buffers
When using buffers, dissolve salts first, adjust the pH, then filter to remove insoluble
material.
Tetrahydrofuran (THF)
When using unstabilized THF, ensure that the solvent is fresh. Previously opened bottles
of THF contain peroxide contaminants, which cause baseline drift.
C.2 Solvent Compatibility
The Waters 1525 HPLC Pump is constructed of high-quality (316) stainless steel
components that, with some minor restrictions, can be used with all solvents. This section
lists the solvents that have and have not been approved for use with the 1525 HPLC
Pump.
Solvents to Avoid
You can use any solvent with the 1525 HPLC Pump. However, long-term static exposure to
halide salts (for example, fluoride, bromide, chloride, and iodide) will pit and corrode
stainless steel parts. When using these salts, flush the system thoroughly with water if the
pump will be idle for more than 2 days.
Solvents to Use
Materials of construction used in the 1525 HPLC Pump are nonreactive with most acids,
bases, salts, and organic solvents.
The solvents in
have been approved for use with the 1525
HPLC Pump. These include salts, acids, and bases in concentrations up to 1 M (unless
otherwise noted), and organic solvents in concentrations of up to 100% (unless otherwise
noted). Higher concentrations can be used in many instances.
Caution:
THF contaminants (peroxides) are potentially explosive if concentrated or taken
to dryness.
Summary of Contents for 1525M
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