14
Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM
CAUSE
SOLUTION
I. Optical Section:
1. The illumination is open, but
the field of view is dark.
The plug of the LED holder
is not connected to the il-
lumination set
Connect them
The brightness is too low
Adjust to a proper setting
Too many colour filters have
been stacked
Minimize the number of the filters
2. The edge of the field of view
is vignetted or the brightness is
asymmetric.
The nosepiece is not in the
correct position
Turn the nosepiece to a click stop
The color filter is partially
inserted
Insert the filter to full depth
The phase contrast slider is
not in the proper position
Move the slider to a click stop
3. Dust and stains can be seen in
the field of view.
There are stains and dust on
the specimen
Clean the specimen
There are stains and dust on
the eyepiece
Clean the eyepiece
4. There is an apparent double
image.
The size of the aperture
diaphragm is too small
Open the aperture diaphragm
5. Poor image quality:
The image is not sharp
The contrast is not high
The details are not clear
The phase contrast is low.
The nosepiece is not in the
center of the light path
Turn the nosepiece to a click stop
The aperture diaphragm in
the view of field is opened
too much or too little
Adjust the aperture diaphragm
The lenses (condenser,
objective, eyepieces are
culture dish) is dirty
Thoroughly clean all the optical system
In phase contrast observa-
tion, the bottom thickness
of the sample is more than
1.2mm
Use a sample holder whose bottom
thickness is less than 1.2mm
A bright field objective is
used for phase contrast
observation
Switch to a phase contrast objective
The condenser ring is not
aligned with the objective
phase ring
Adjust the condenser ring to match the
objective phase ring
The light ring and/or the
phase contrast ring is not
centered
Adjust the bolts to center them
The objective used is not
compatible with the phase
ring
Please use a compatible objective
The phase contrast depends
on the sample position
The sample holder is not flat. Move the
sample around until a compatible area
is found.
Summary of Contents for VisiScope 400 Series
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