54
vwr.com I Instruction manual VWR
®
CO
2
incubator
Problem
Cause
Solution
The unit is not working
Check the voltage in the socket.
M
easure the voltage in the socket. Plug the unit into
a different socket, preferably from a different
electrical circuit.
Call an electrician with qualifications to inspect
the electrical wiring.
Check if the unit is plugged in.
Plug in the unit.
Check the status of a circuit-breaker on the back
of the unit.
Press the circuit breaker on the back of the unit.
Check if the power cable is broken.
Change the cable.
The unit is not heating
up
Check if the door of the unit is closed properly.
Clean the gasket.
Does the installation site meet the installation
conditions?
Adapt to the installation requirements (according to
CO
2
measurement
malfunction
Check if water is not condensed in
the measuring tip of CO
2
sensor.
Remove the white filter from the measuring tip of
CO
2
sensor (in the chamber) and let the tip to dry or
run the program without water pan e.g. 50
o
C till
the sensor is dry.
CO
2
concentration is
not rising
Check if the gas cylinder is not empty.
Replace the CO
2
cylinder.
Check if the gas hose is not curved too much.
Straighten out the gas hose.
Check if the CO
2
gas filter is not clogged.
Replace the filter (according to
Check if the CO
2
gas filter was replaced
according to schedule.
Check the CO
2
pressure on the pressure
regulator (approx. 1,5-2 bar).
Adjust the pressure regulator for proper CO
2
pressure (approx. 1,5-2 bar).
Service of the unit
15.
Replacing the CO
2
gas filter
15.1.
When the chamber is in operation, the incoming gas passes through a sterile filter (aseptic filter, filtration efficiency 99,99%,
particle size 0,2
m). The gas cylinder and the supply hose can be a source of dirt. Therefore the inlet filter prevents dirt
accumulation in the gas inlet valves and the tubes leading into the inner chamber.
When using gas with a technical grade of 99,5%, it is recommended to change the CO
2
gas filter
once a year
. When using
gases with less pureness, the changing intervals should be shorter.
A safe CO
2
gas filter replacement should be performed according the following procedure:
1.
Turn off the unit and remove the cover on the chamber rear.
2.
Remove the gas filter on both sides from the gas hoses and then install a new filter.
3.
Check gas connections for leaks (e.g. with leak spray or dilluted soap solution). Run an exemplary program, adjusting
5% CO
2
concentration. When no gas bubbles are observed after few valve openings, that can be heard (max. 1 minute
of the program), the hoses are connected properly. Stop the program.
