14
vwr.com I Instruction manual VWR
®
CO
2
incubator
Connection of the CO
2
gas cylinder
3.7.
The manufacturer supplies the unit with a 3m pressure gas hose with pre-assembled hose nozzle for quick connector and a hose
clamp. This hose is used to connect CO
2
cylinder.
To establish the gas cylinder connection to the chamber:
1)
place the CO
2
cylinder vertically and attach it to the wall with a special clamp, rope or chain,
2)
ensure the correct CO
2
output pressure of 1,5-2 bar (use a pressure reducer),
3)
connect the supplied gas hose to the pressure reducer of the gas cylinder or central gas supply and secure
the connection with the supplied hose clamp,
4)
connect the pre-assembled hose nozzle of the gas hose to the quick connector on the chamber rear,
5)
slowly unscrew the valve of the gas cylinder while checking low leakage connection.
Leak test
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or dilluted soap solution).
The gas outlet pressure MUST NOT exceed the indicated value of 2,5 bar. The pressure above
will result in chamber damage.
Before connecting, check the outlet pressure on the pressure reducer of the cylinder. Adjust
the outlet pressure to 1,5 – 2,0 bar above the ambient pressure.
Only use the supplied hose nozzle to connect to the quick connector. Otherwise, the quick connector may leak, and/or it may
become impossible to connect the original hose nozzle. In this case, please contact VWR Service.
The CO
2
gas cylinder is NOT supplied with the CO
2
incubator. The purchase, transport
and connection is the responsibility of the user.
The gas connections must be installed by qualified personel who are trained in handling
the respective gases and familiar with the required safety measures.
During sterilization process the inner chamber of the incubator is hot (do not touch the glass door
or inner parts of the chamber).
