V&P Scientific, Inc.
7
Technical Note 378B
USING A 1L SpinVessel
®
SYSTEM
The optimal stirring mode is dependent upon the application and needs to be empirically
determined.
Factors to consider in determining optimal 1L SpinVessel® operation are the density of
the particulates, their size and shape, as well as the volume and viscosity of the liquid. Start with 150
RPM and 2.1 rotations before reversing direction, then test to determine the appropriate RPMs and
number of rotations for the particulate’s density, fragility, and solution viscosity.
We have found that
smaller circumference SpinVessels® require higher RPMs than larger circumference SpinVessels,
because the critical factor is the linear speed generated at the circumference not the RPMs. See page
501 of our
for a discussion of this phenomenon.
The following settings are ones to use when first testing the VP 418SV-1-1L-FB-CC SpinVessel®:
Speed: 150 RPM
Rotate: 2.1 Pause: 200 milliseconds
Ramp: 10
It is always a good idea to practice with water in the SpinVessel® to determine safe RPM, rotation,
pause and ramp numbers before using expensive reagents.
Note that, if Ramp is set to 0, water will splash out of the 1L Spin Vessel.
The VP 418SV1-1L-FB-CC SpinVessel® is designed to be used only with a
VP 830SV-1L-FB SpinVessels®
tube. Please contact V&P Scientific for pricing.
See below for an example of low versus high RPM for a solution of yeast cells in a large circumference
6 liter SpinVessel®. Note: That when given a longer mixing time even the slower speed (20 RPM for
more gentle mixing) was still able to suspend the yeast cells.
0.00
0.20
0.40
0.60
0.80
1.00
1.20
2
5
9
14 18 24 28 33 37 40 44 60 90 120 230 480 720
Ab
so
rb
an
ce
Time (Seconds)
Yeast Resuspension with 6L SpinVessel®
20 RPM vs 100 RPM
20 RPM, 4 rotations, 500 pause
100 RPM, 4 rotations, 500 pause
