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A gamma curve is smooth: there are no unexpected jumps or cut-offs. This means that when viewing a gamma adjusted
image, you will be able to see the details (intensity differences) in both the black and white areas of the image.
When the bright areas of these types of images are correctly exposed, the darker areas can be so dark that they are in
effect invisible. Gamma Adjust can remedy this problem. The gamma adjustment results in a better display of detail by
lightening the darker areas without burning out bright areas or lightening black areas:
• Inverse the image
Select “Inverse the image” to inverse the grey level of the image. This makes a negative image.
• Display the sat
uration
A saturated image is inappropriate for image quantification with image analysis software. The saturation option allows
you to visualize in red, pixels that have the maximum grey level in order to avoid flattened peaks.
The maximum grey level depends of the bit-depth. For instance:
A 16-bit image has a maximum grey level of 65535
Select the saturation option and the saturated pixels are displayed in red:
Note
: If an image is being acquired and the «Saturation» option is checked, the modification is applied to the current
acquired image
Note
: A saturated image creates quantification error when studied by an image analysis software. Gel-doc systems have
to indicate to the user if the image is saturated and if it is then necessary to modify the integration time.
• Select the color palette
The pseudo colors can display different types or levels of fluorescence in an image. It replaces the original grey levels of
the image by another palette color. The software has several predefined palette designs. Select your palette design from
the followings:
