Aqua
Fluor
®
Handheld Fluorometer and Turbidimeter
December 15, 2015 P/N: 998-0851 Revision 1.7
TURNER DESIGNS
845 W. Maude Ave. Sunnyvale, CA 94085 Phone: (408) 749-0994 FAX: (408) 749-0998
Page 1: ...AquaFluor Handheld Fluorometer and Turbidimeter December 15 2015 P N 998 0851 Revision 1 7 TURNER DESIGNS 845 W Maude Ave Sunnyvale CA 94085 Phone 408 749 0994 FAX 408 749 0998 ...
Page 2: ... Diagnostic Information 10 3 Data Transfer and Internal Data Logging IDL 3 1 Shipping Checklist 11 3 2 Hardware Requirements 11 3 3 Installation 11 3 4 Connecting 11 3 5 Real Time Data Transfer 11 3 6 Data Transfer Troubleshooting 12 3 7 Internal Data Logging IDL 12 4 Sample Analysis Guidelines 4 1 Handling Samples 13 4 2 Positioning Samples 13 4 3 Data Quality 13 5 Applications 5 1 In Vivo Chloro...
Page 3: ...end of life product proceed as follows If you purchased your instrument through a Turner Designs Distributor please contact your local representative They will instruct you where to return the end of life product If you purchased your instrument directly from Turner Designs please contact Turner Designs Customer Service By Phone 1 408 212 4041 or Toll Free 877 316 8049 By Email Customer Service at...
Page 4: ...figured with any two channels as follows in vivo Chlorophyll Extracted Chlorophyll Cyanobacteria Phycocyanin or Phycoerythrin Turbidity Rhodamine WT Fluorescein Ammonium CDOM FDOM or Optical Brighteners 1 2 Quick View Diagrams 1 3 Inspection and Setup 1 3 1 Inspection Upon receiving your instrument please inspect everything carefully and make sure all accessories are present All shipments include ...
Page 5: ...f glass or quartz cuvettes exceeds the 12 mm maximum outer dimension of the AquaFluor The 10 mm size of most glass or quartz cuvettes is the internal dimension and some plastic cuvettes may also be too large Do not force oversized cuvettes into the sample compartment This can damage the sample compartment If the cuvette does not easily fit down inside the AquaFluor you will need to purchase differ...
Page 6: ... Calibration Overview The AquaFluor can be calibrated using a Primary standard A Primary Standard is one that contains the same fluorescent material that you are measuring in your unknown samples When a primary standard of known concentration is used for calibration and the STD VAL is set to reflect that concentration the AquaFluor will give an actual quantitative concentration reading For example...
Page 7: ...librate with a primary standard that has a concentration of 50 µg L then you will set the value to 50 and the resulting unit of measure will be in µg L 1 Press the STD VAL button 2 Use the and arrow buttons to set the standard value Pressing the arrow button once quickly will make a small incremental change to the value Holding either arrow button will allow you to change the value using fast scro...
Page 8: ...al that is intended to give you consistent repeatable readings Please refer to Appendix A for the solid standard configuration for your application When a Secondary Standard is used the AquaFluor will give relative sample readings that are proportional to the measured fluorescence In some cases these relative sample readings will be correlated back to actual concentrations that are determined late...
Page 9: ...reads the concentration value of interest Record the secondary standard reading for future reference 7 At this point remove the solid standard and turn the locking setscrew clockwise until it just makes contact with the attenuation screw NOTE DO NOT OVERTIGHTEN or remove either hex screw on the Solid Secondary Standard overtightening may damage the standard 8 Proceed with analyzing your samples 9 ...
Page 10: ...he accuracy of your results Refer to Section 4 for more details 2 Press either READ button The instrument will measure and average the fluorescence signal for approximately 10 seconds 3 The reading result will be displayed on the top line of the Home screen 4 The top left corner will then display WAIT for 5 seconds Once WAIT disappears another sample reading can be performed 2 7 Diagnostic Informa...
Page 11: ...puter only has USB ports we recommend purchasing a USB to serial adapter cable Communication and Integration Cable P N 2300 155 can be used as a standard USB to Serial Adaptor Cable 2 Plug the opposite end of the cable into the port at the base of the AquaFluor 3 Open the Spreadsheet Interface software 4 Click on the box to the right of the COM port icon to select the appropriate COM port 5 Click ...
Page 12: ...nections Make sure both ends of the cable are plugged in tightly 3 7 Internal Data Logging IDL The Internal Data Logging option allows the downloading of stored data from the AquaFluor The AquaFluor can log up to 1000 data points The DATA screens control logging downloading and erasing the data as described below Activate Data Logging 1 Press the DATA button 2 times 2 Press ENT to toggle between l...
Page 13: ...o help dissipate bubbles 4 2 Positioning Samples The orientation of the cuvette in the sample compartment can give slightly different readings especially for low concentration samples This is due to variations in the walls of the cuvette that are not readily visible to the eye We recommend that the cuvette be marked at the top on one side and positioned in the sample compartment the same way each ...
Page 14: ...uorescence data must be correlated with extracted chlorophyll data that can be obtained through the extraction and measurement of the pigment from grab samples on a laboratory fluorometer spectrophotometer or HPLC When collecting grab samples for chlorophyll extraction the in vivo reading must be noted at the same time the sample is collected Several samples should be collected within each niche o...
Page 15: ...easurement when investigating Nitrogen cycling and nutrient dynamics Historically methods for ammonium determination have been a source of frustration within the scientific community due to the lack of a simple accurate and affordable method particularly for measurements in the submicromolar range The ammonium technique offers researchers and technicians an excellent alternative to the existing co...
Page 16: ...uaFluor and Rhodamine dye is detected using the Green channel The linear detection range for both dyes is 0 4 to 400 ppb active ingredient in potable water For more information on tracer dye use visit Turner Designs website under Applications 5 6 Cyanobacteria Monitoring The Cyanobacteria channel of the AquaFluor detects the fluorescence of either phycocyanin PC or phycoerythrin PE pigments unique...
Page 17: ...r Agents OBAs are primarily added to laundry soaps detergents and cleaning agents for the purpose of brightening fabrics and or surfaces Laundry wastewater is the largest contributor of OBAs to wastewater systems because it retains a large portion of dissolved OBAs Water municipalities and researchers are evaluating OBA concentrations in lakes rivers and coastal ocean to determine the efficiency o...
Page 18: ...ervice To obtain service during the warranty period the owner shall take the following steps 1 Write email or call the Turner Designs Technical Support department and describe as precisely as possible the nature of the problem Phone 1 877 316 8049 Email support turnerdesigns com 2 Carry out any adjustments or tests as suggested by the Technical Support Department 3 If proper performance is not obt...
Page 19: ... can assist you by phone or correspondence we will be glad to at no charge Repair service will be billed on a fixed price basis plus any applicable duties and or taxes Shipment to Turner Designs should be prepaid Your bill will include return shipment freight charges Address for Shipment Turner Designs 845 W Maude Avenue Sunnyvale CA 94085 ...
Page 20: ...AquaFluor User s Manual 20 ...
Page 21: ...quired for Extracted Chlorophyll where solvents are used Requires Adapter PN 8000 932 10 029A Minicells Borosilicate Glass 5 x 31mm Flat bottom For use with high concentration samples Requires Adapter PN 8000 936 200 µL capacity 7000 950 AquaFluor Size 1 75 x 3 5 x 7 25 4 45cm x 8 9cm x 18 4cm Weight 13 9oz 0 4kg Resolution 12 bits LCD Display 2 x 16 characters Case Meets IP 67 Standard dustproof ...
Page 22: ...2 7000 959 7000 957 10 029A Not Available 8000 411 Phycoerythrin Marine Cyanobacteria 8000 952 7000 959 7000 957 10 029A Not Available 8000 409 Rhodamine Dye 8000 952 7000 959 7000 957 10 029A 0 026 C Exponential 8000 408 Turbidity Not Available 7000 957 10 029A Not Available 7000 959 Methacrylate 10x10 mm cuvettes 7000 957 Polystyrene 10x10 mm cuvettes 10 029A Glass 12x75 mm test tubes Readings o...
Page 23: ...ncreasing This effect is known as sample quenching and is due to light absorption losses in the sample See the Figure 1 graph below When you start to see visual color in the sample this is an indicator that the sample may be above the upper limit of linearity The linearity can be verified by diluting a sample 1 1 or another convenient dilution ratio If the sample is within the linear range the rea...
