ThermoFisher Scientific NanoDrop Eight, User Manual

Page: 138 / 152

8 Maintenance
Instrument Diagnostics
138 NanoDrop Eight User Guide Thermo Scientific
Performance Verification
Run Performance Verification every 6 months to confirm pathlength accuracy is
within specifications.
A CHEM-PV-8 kit containing two vials of PV-1 {aqueous nicotinic acid (C
6
H
5
NO
2
),
potassium nitrate (KNO
3
)} is required to verify the performance of the Thermo
Scientific™ NanoDrop™ Eight microvolume UV-Vis spectrophotometer.
Supplies needed
• lint-free laboratory wipes
• deionized water (DI H
2
O)
• cCalibrated Precision 8-channel Pipettor (0.5 - 10 µL)
• CHEM-PV-8 kit containing two ampoules of PV-1, two 8-well PCR strip tubes,
and transfer pipettes
• laboratory gloves
Before you begin
First make sure the pedestals are properly conditioned. To test pedestal
conditioning, clean the upper and lower pedestal surfaces with a dry, lint free
laboratory wipe, then pipette 1.5 µL DI H
2
O onto the eight lower pedestal surfaces.
The droplets should “bead up” as shown below. If it does not, recondition the
pedestals . Remove the water sample from the upper and lower pedestal surfaces
with a dry laboratory wipe.
Note The PV-1 solution comes in a single-use ampoule. Before you open the
ampoule, shake it vigorously and then allow the liquid to collect in the bottom
portion of the ampoule. After the ampoule is opened, its contents must be used
within one hour. Pipette directly from the ampoule; do not transfer the solution.
Droplets “bead up” on properly
conditioned pedestals