ThermoFisher Scientific MGS108 User Manual Download Page 16

One Lambda, Inc. | A Thermo Fisher Scientific Brand

  

Micro SSP Gel System User Manual

TDX-OLI-DMR-PS-0619 Rev. 02

14

Trouble Shooting

These troubleshooting tips cover only electrophoresis-related problems using the Micro SSP Gel System.  
Problems related to signal intensity and quality could also be caused by sample quality/quantity, pre-
PCR or other sample manipulations, or photography.  Please refer to the appropriate product manuals or 
contact your One Lambda representative if problems persist.

Problem

Possible Cause

Remedy

No current or no movement of 
the dye front

Power is not on

Turn on power supply. Check for 
formation of bubbles at the 
electrode.

Power supply is not properly 
adjusted

Check gel and buffer levels.  

The safety switch in the Cover is 
not engaged

Place the Cover down firmly by 
pressing it down until a click can 
be heard (this turns on the safety 
switch).

Check for proper operation of the 
safety switch.  The metal screw 
in the base unit must be present 
and correctly adjusted to activate 
the safety switch.

Not enough buffer

Make sure there is enough buffer to 
fill all wells in the gel.

Dirty electrodes

!

Turn off the power supply and 

disconnect both electrical leads 
before handling the electrodes.

Make sure the platinum 
electrodes are free of salt 
deposits, dust, or grease. Gently 
wipe clean with disposable wipes 
and distilled water and DO NOT 
attempt to remove electrodes.

Breakage in electrical wiring

Check for broken or loose parts in 
the electrical leads and the electrode 
assembly.  If found, DO NOT attempt 
to correct, contact your One Lambda 
representative immediately.

Summary of Contents for MGS108

Page 1: ...TDX OLI DMR PS 0619 Rev 02 User Manual Micro SSP Gel System Catalog MGS108...

Page 2: ...Hannover Germany 2017 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified Specifications terms...

Page 3: ...Symbols 3 Micro SSP Gel System Preparation 5 Electrophoresis Procedure 8 Cleaning and Safety Precautions 11 Cleaning 11 Gel Box Cleaning Precautions 11 Power Rating 11 Electrical Safety Precautions 12...

Page 4: ...onal lane total of 108 sample wells Same footprint as a standard 96 well microtiter plate Low reagent volume requirement Approximately 3 minute run time A leveling bubble and adjustable leveling knobs...

Page 5: ...Description Catalog 1 Gel Box MGS XGB9 1 Gel Comb Holder MGSCH9 1 Gel Box Cover MGS XC Symbols CAUTION Refer to safety information in accompanying documents If the Micro SSP Gel System equipment is u...

Page 6: ...SP Gel System User Manual TDX OLI DMR PS 0619 Rev 02 4 Class II Equipment Direct Current DC Voltage Manufacturer Serial Number Read Usage Instructions Waste Electrical and Electronic Equipment WEEE Im...

Page 7: ...m for any cracks or damage that may have occurred during transport 2 Pour water into the Gel Box as you would with buffer and look for any evidence of leakage Empty and dry the Gel Box CAUTION Discard...

Page 8: ...he leveling bubble this assures a level surface figure 4 The adjustment thumbscrews should not be disturbed after it has been leveled Figure 4 Leveling bubble 7 Orient and fully insert all 14 Gel Comb...

Page 9: ...an pour a gel with an even thickness over the entire surface of the Gel Box Ensure that the agarose covers the entire surface evenly This may be done by tilting the Gel Box and Base back and forth to...

Page 10: ...ading Important Amplified DNA samples should be loaded immediately after buffer is poured Ensure all wells are filled and the gel is evenly covered with buffer The Gel System should be used by trained...

Page 11: ...rn on the power supply and set the voltage according to kit product insert Turn off the power supply 4 While aligning the color corded sides place Cover onto the Gel box such that the bottom of the Co...

Page 12: ...ON Be attentive of any evidence of leakage from the system If any leakage is detected immediately turn off the power supply Do not move Gel System while running test 8 Turn off the power supply loosen...

Page 13: ...ce of the product Dry with a clean damp cloth or towel To clean the Cover lightly dust do not rub the surfaces with a soft clean cloth Then carefully wipe the surfaces with a soft wet cotton terry clo...

Page 14: ...Gel System If any leakage is detected immediately turn off Power Supply Once finished remove the Cover turn off power supply return voltage setting to zero and disconnect leads from the power supply...

Page 15: ...For service requests contact Technical Support Only to be supplied by One Lambda A Thermo Fisher Scientific Brand Components must be disposed of in accordance with WEEE Directive Description Cat No Ge...

Page 16: ...gel and buffer levels The safety switch in the Cover is not engaged Place the Cover down firmly by pressing it down until a click can be heard this turns on the safety switch Check for proper operati...

Page 17: ...Prepare fresh batch of reagent and protect it from light Prepare only enough fresh agarose solution for 1 2 days worth of work Not enough fluorescence Wear appropriate PPE when performing UV Photogra...

Page 18: ...ke sure each sample well and electrode well is filled with running buffer before loading samples Not enough samples loaded Load all PCR samples to each well Sample lost during loading A mechanical pip...

Page 19: ...trophoresis after the dye has migrated 0 5 cm into the gel UV light bulb in the trans illuminator is out of order or weak Check and if necessary replace UV light bulb s according to the manufacturer s...

Page 20: ...ng and other breakage Replace as required Undissolved agarose Make sure agarose is completely melted Gel ran too fast Do not exceed recommended voltage Evaporation of running buffer Use a gel as soon...

Page 21: ...e gel Difficult to visualize bands Incorrect background color Use a gray or black background Excessive leaking of samples when a multi channel pipette is used Leaking tips or tips not aligned Make sur...

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