Doc No.
2
November 2018
4 mm Chromatography
2 mm Chromatography
P/N: 045877
P/N: 045878
1 Coil = 1.5
–
3.0 mL/min
1 Coil = 0.37
–
0.75 mL/min
2 Coils = 0.5
–
1.5 mL/min
2 Coils = 0.12
–
0.37 mL/min
P
3
, ________ psi
□
Calculate the backpressure across the backpressure coil(s) (
BP
2
):
BP
2
, =
P
3
–
P
2
= ________ psi
Pass:
30-40 psi for carbonate eluent or 10-40 psi for other eluents
If the back pressure exceeds the specification, see
Backpressure Coil Adjustment
section
□
II.
Hydration & Backpressure Check of Suppressor
Before putting the new suppressor to use, hydrate it properly first. Improper hydration
will result in irreversible damage to suppressor.
This will void the warranty!
Step 4:
Connect the
column out
tubing to the suppressor
Eluent
Inlet
and direct
the suppressor
Eluent Outlet
to the
Regen Inlet
of the suppressor using ~
6”
of
green PEEK tubing
(ID 0.030”)
provided with the system ship kit. Direct the
suppressor
Regen Outlet
to waste (see
Figure 1
below).
□
T
urn on the pump and flow ≤10
mM eluent or DI water at
0.5 mL/min
for 4mm
suppressor or
0.125 mL/min
for 2mm suppressor for 20 minutes.
The power to
the suppressor must be turned off during this step.
□
Figure 1:
Suppressor Hydration Configuration
