Thermo Scientific TX-750, Instruction Manual

Page: 37 / 57

5
Aerosol-tight Applications
Basic Principles
5-2
75003607 TX-750 Thermo Scientific
Basic Principles
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Check that the sample containers are well suited for the desired centrifugation process.
Fill Level
The tubes are only to be filled to a level which ensures that the sample is unable to reach the top of
the tube during centrifugation. Therefore fill the tube only 2/3 of the rated level.
Checking the aerosol Tightness
The aerosol tightness testing of the rotors and buckets took place according to the microbiological
test process in accordance with the EN 61010-2-020 Appendix AA.
Whether or not a rotor is aerosol-tight depends primarily on proper handling.
Check as needed to make sure your rotor is aerosol-tight.
The careful inspection of the seals and seal surfaces for signs of wear and damage such as cracks,
scratches and embrittlement is extremely important.
Aerosol-tight applications are not possible if the bucket tops are open.
Aerosol-tightness requires the correct operation when filling the sample vessels and closing the rotor
lid.
Quick test
As a quick test, it is possible to test the aerosol-tight beaker and fixed-angle rotors using the following
process:
1. Lubricate all seals lightly.
Always use the special grease 7600 3500 when lubricating the seals.
2. Fill the bucket or the rotor with approx. 10 ml of carbonated mineral water.
3. Close the bucket or the rotor as explained in the handling instructions.
4. Shake the bucket or the rotor vigorously using your hands.
This releases the carbonic acid gas which is bound in the water, resulting in excess pressure. Do
not apply pressure to the lid when doing so!
Leaks can be detected by escaping water or the sound of escaping gas.
CAUTION
When centrifuging hazardous samples, do not open aerosol-tight rotors or
buckets unless placed in a safety cabinet.
Always bear in mind the maximum permitted fill levels.
Be sure to check all seals before starting any aeorsol-tight applications.