Thermo Scientific thermoscientific Vanquish Pumps C, Operating Manual

Page: 237 / 296

10 • Modifying the Pump for Specific Applications
Pumps C, Pumps F ( VC-Pxx, VF-Pxx)
Operating Manual Page 237
In the standard configuration, the pump has been optimized for the
dwell volume and ripple. You can adapt the dwell volume or ripple to
your requirements by replacing the mixing system with which the pump
is shipped with a mixing system with a different volume:
• Mixing system with lower volume
Operating the pump with a lower volume mixing system reduces the
dwell volume of the pump and thus, the gradient delay volume of
the system but increases the ripple.
Consider installing a mixing system with lower volume for gradient
separations at low flow rates (for example, less than 100 µL) or for
very steep gradients.
• Mixing system with higher volume
Operating the pump with a higher volume mixing system reduces
the ripple but increases the dwell volume of the pump and thus, the
gradient delay volume of the system.
For information about the available mixing systems, see Available Mixing
Systems ( } page 237) .
10.1.1 Available Mixing Systems
Each mixing system comprises a static mixer and a capillary mixer. The
volumes of both mixers determine the entire volume of the mixing
system.
The table lists the available mixing systems:
Description Remarks
Set inline filter, volume: 35 µL, comprising:
• Inline filter (static filter), volume: 10 µL
• Capillary mixer, volume: 25 µL
Requires also a different
capillary to connect the pump to
the autosampler.
Mixing system, volume: 100 µL, comprising:
• Static mixer, volume: 75 µL
• Capillary mixer, volume: 25 µL
Requires also a different
capillary to connect the pump to
the autosampler.
Mixing system, volume: 200 µL, comprising:
• Static mixer, volume: 150 µL
• Capillary mixer, volume: 50 µL
Binary VF-pump, isocratic pump :
Mixing system with which the
pump is shipped.
With the isocratic pump, the
mixing system serves as inline
filter.
Other pumps: See TIP at the end
of this table.