Thermo Scientific TCF-32 Instruction Manual Download Page 1

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Continuous Flow Rotor
TCF-32

Instruction Manual

english

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Summary of Contents for TCF-32

Page 1: ...uous Flow Rotor TCF 32 Instruction Manual english Analyze Detect Measure Control The original copy of this manual is valid Thermo Scientific cannot guarantee the accuracy or completeness of subsequent copies ...

Page 2: ...rmination of sample flow rate 26 4 5 Lubricating unit 26 5 PRECAUTIONS IN USING ROTOR 28 6 TROUBLE SHOOTING 31 7 LIFE AND WARRANTY OF ROTOR 33 8 WARRANTY OF LUBRICATING UNIT AND SEAL ATTACHMENT ASSEMBLY 34 9 MAINTENANCE 35 10 SEPARATION CHARACTERISTICS 38 11 COMPONENTS AND ACCESSORIES 39 12 INSTRUCTION FOR 940ML CORE 44 13 Preventive Maintenance 47 ...

Page 3: ...manual the allowable speed should be reduced by 10 Set a rotor gently and securely onto the drive shaft hub Practice setting repeatedly to acquire the knack Confirm that the rotor is free from corrosion and scores If white corrosion is recognized on a tube hole bottom of an angle rotor or a hole bottom of a bucket for swing rotor immediately stop using it and contact the nearest agent of Thermo Sc...

Page 4: ...t assembly are assembled Do not rotor at a speed of more than 3 000 rpm unless the rotor is filled with liquid If the above cautions are not observed shaft D could be broken or the core could be damaged Always ground the ultracentrifuge Iubricating unit and low temperature circulating chamber before use Be sure to read article on 9 MAINTENANCE ...

Page 5: ...lubricating unit AC 100 240V 1A 50 60Hz NOTES For operation of Model TCF 32 the following apparatus are required besides the standard configurations of Model TCF 32 and Preparative Ultracentrifuge which have to be prepared for installation of Model TCF 32 at customer s site 1 Sample Loading Pump Specifications Flow rate Has to be as fast as operating flow rate required for separation of samples Ex...

Page 6: ...n Lubricating unit Ass y Seal Attachment Ass y Rotor body ass y Sample outlet Sample inlet Water outlet Oil discharged from bearing is sucked and recovered Cooling water is supplied Oil outlet Oil inlet Water inlet Door adapter Bearing Bearing housing Shaft D Bearing housing Seal body ass y Collar Seal Door Rotor ...

Page 7: ...ed in the rotor The core forms a passage of sample and density gradient solution in the rotor Its material is Noryl The titanium sleeve is for reinforcing the core and is put on the outside of the core It is made of titanium alloy The blades are for preventing the sample and density gradient solution in the rotor from whirling up It is made of Noryl see Fig 3 Fig 2 Rotor body assembly Fig 3 Core t...

Page 8: ...eal tube head and seal The shaft area of seal tube head is inserted in the polyphenylene oxide resin shaft up to the core and serves as a passage for sample etc It is fastened on the shaft with left hand threads The material is stainless steel The seal is mounted on the top of the seal tube head It is an important component in charge of letting in and out sample etc to and from spinning rotor The ...

Page 9: ... Seal body assembly collar and screw sockets The seal body assembly consists of seal piece which is in contact with the rotor seal and transfers sample etc pipe spring manifold and connector The seal body assembly is pushed against the seal by the spring As for materials the seal piece is made of tungsten carbide and others are made of stainless steel The collar which is made of stainless steel is...

Page 10: ...DRAIN collection 3 Malfunction detection l Lubricating oil flow detection The pressure detection formula l Cooling water flow detection The pressure detection formula l It is an extraordinary caution when Lubricating oil doesn t flow l It is an extraordinary caution when cooling water doesn t flow 4 Malfunction warning There is a warning in the electronic buzzer 5 The container The glass bottle of...

Page 11: ...ing below the half of the bottle replenish 4 The switch of the control panel has the following function l The OIL switch is the ON OFF switch of the lubricating oil pump Also it is the ON OFF switch of the lubricating oil flow abnormal detection equipment When the flow of lubricating oil stops it informs in the buzzer sound When making this switch OFF the buzzer sound stops l The WATER switch is t...

Page 12: ...dapter e Screwdriver fastens bearing housing f Stopper B prevents bearing housing from rotating g Seal tube head setter tightens and removes seal tube head h Seal extractor detaches seal i Core setter extracts core j Pressure gage supervises sample injection pressure k Clamp changes over pipe passage l Watchmaker s screwdriver removes O ring m Silicone grease maintains rotor body n Lubricant for s...

Page 13: ...adient solutions from lighter ones Inject high density extrusion solution and recover separated solution Pelleting Inject buffer solution Continuously inject sample at specified flow rate Stop and disassemble rotor and recover CAUTION This rotor should never be spinned at a speed over 3 000 rpm unless all the parts such as rotor assembly seal attachment assembly and lubricating unit assembly are m...

Page 14: ...tc are free from scratches and apply a thin coat of vacuum grease on them For installation of the seal packing and metal ring refer to the magnified figure Make sure the rotor and cover are free from cracks or the like which is a symptom of material fatigue Warning Do not remove the overspeed adapter stripe ring mounted on rotor bottom Caution Since O ring and seal packing which are used in the ro...

Page 15: ...fore installation Put the blades on the pins on titanium sleeve Position the sample outlet hole in the core and the titanium sleeve in relation to each other as shown below 3 Introduce in the rotor the assembly of core titanium sleeve and blades Sample outlet hole Sample inlet hole Blade Blade Titanium sleeve O ring 1AP135 O ring 1AG145 O ring 1AF3 Core ...

Page 16: ...he cover Make sure the O rings are free from scratches and apply a thin coat of vacuum grease on them The shaft D can be introduced 15 mm in the cover Put it in until it is held against Two O rings are used on the top and bottom ends of the shaft D 6 Sufficiently tighten the swageloknut until the clutch of the torque wrench functions It functions at 5 N m 51kgf cm Nut Match mark Shaft D O ring sto...

Page 17: ...racentrifuge turn on the POWER switch and open the door To remove the door hole cover use the door hole adapter handle 2 Install the rotor gently on the spin shaft 3 Close the door Apply a thin coat of vacuum grease on the door seal O ring and mount it back in position O ring ...

Page 18: ...e rotor is positioned approximately at the center of the center hole of the door adapter If the eccentricity is too great the door may have been set to the incorrect position Return to step 3 and close the door firmly 5 By means of the furnished screwdriver loosen 2 3 turns six setscrews for stopper A of door adapter Screwdriver Shaft D Stopper A ...

Page 19: ...es the door adapter Then turn about 60 to engage the stopper with the door adapter in such manner that the water passage faces the rear of the centrifuge 8 Position the bearing housing stopper B which prevents the bearing housing from turning in the gap between the bearing housing and door adapter Water passage a Connect to OIL IN Stopper B b Connect to DRAIN IN c Connect to WATER OUT d Connect to...

Page 20: ...the rotor Inspect the assembled state of the bearing housing and rotor shaft and the bending of the shaft and correct If required 3 When the rotor attains a speed of more than 950 rpm turn on the VACUUM switch to vacuumize the rotor chamber 4 After 30 60 seconds cancel the ZONAL mode and stop the rotor 10 Using the screwdriver tighten the six stopper A setscrew loosened in 5 11 Insert the seal tub...

Page 21: ...when removing the seal The top end of the seal is an important section which prevents the liquid from leaking If this end is damaged a defect such as a leakage of liquid will occur Take great care when handling this section 13 Connect tubes to the seal body assembly to make it ready for mounting Pressure gage Pump Tee Screw socket B Water connector Screw socket A Installing O rings on Seal Tube Ou...

Page 22: ...ater passage of the bearing housing This is necessary so the cooling water can flow smoothly for efficient cooling 15 Introduce the seal body assembly gently in the bearing housing and tighten by turning the screw socket A clockwise while pushing the screw socket B downward At this time take care not to move the bearing housing If it is dislocated shaft D will become eccentric Perform readjustment...

Page 23: ...seal assembly to check that there is no leakage of cooling water 17 Turn the screw socket B of the seal body assembly to adjust its height to the position of the red line on the pipe The upper surface of manifold A should be positioned so that some of the red line is still visible If the red line is invisible even by tightening the screw socket B fully the screw socket A may not have been tightene...

Page 24: ... 5 kgf cm2 This is not abnormal and can be corrected by filling with the overlay solution in the subsequent stage 3 After the density gradient solution etc are injected change the piping as illustrated and allow overlay solution from the central connector at a flow rate of about 30 ml min When overlay solution overflows from the central connector the density gradient solution has been injected com...

Page 25: ...e inconvenience cannot be eliminated by such measures decelerate the speed to 3 000 rpm and carry out the above steps to eliminate air Caution During high speed revolution check the vacuum degree and if it has dropped unusually or an anomaly has occurred stop operation immediately Poor vacuum may be caused by It is better that no bubbles etc are mixed in the sample 6 After the sample is injected f...

Page 26: ...olution to the external wall of the rotor At this time fill the tube to be injected with extrusion solution Use an extrusion solution the density of which is higher than the density gradient solution or cushion solution injected into the rotor 2 Send extrusion solution from the outer connector and push out the separated sample and density gradient solution from the central connector Thereafter sto...

Page 27: ...g the swagelok nut Install the rotor on the Rotor base and remove the cover Pull out the core by means of the core handle Here recover pellets if any 5 As for the centrifuge clean the chamber interior close the door mount the door hole cover and turn off the power switch Although a small amount of oil leaks into the chamber this is normal 4 3 Capacity in rotor Fig 13 and 14 show the relationship b...

Page 28: ...operates the mechanical pump Lubricant is delivered from the OUT connector and returns to the OIL bottle through the IN connector The oil level in the OIL bottle is becoming the half of the bottle replenish The OIL switch also serves as a safety feature actuating switch If lubricant stops flowing with the OIL switch ON the TROUBLE lamp comes on it inform in the buzzer sound 2 WAER The cooling wate...

Page 29: ...p and DRAIN bottle Turning on the DRAIN switch starts the vacuum pump which sucks and recovers to the DRAIN bottle waste oil discharged from the bearing housing Please check the waste oil quantity of the DRAIN bottle after operation If the reading is high throw away waste oil ...

Page 30: ...nt solutions from lighter ones Inject high density extrusion solution and recover separated solution Pelleting Inject buffer solution Continuously inject sample at specified flow rate Stop and disassemble rotor and recover CAUTION This rotor should never be spinned at a speed over 3 000 rpm unless all the parts such as rotor assembly seal attachment assembly and lubricating unit assembly are mount...

Page 31: ... O ring etc are free from scratches and apply a thin coat of vacuum grease on them For installation of the seal packing and metal ring refer to the magnified figure Make sure the rotor and cover are free from cracks or the like which is a symptom of material fatigue Warning Do not remove the overspeed decall stripe ring mounted on rotor bottom Caution Since O ring and seal packing which are used i...

Page 32: ... installation Put the blades on the pins on titanium sleeve Position the sample outlet hole in the core and the titanium sleeve in relation to each other as shown below 3 Introduce in the rotor the assembly of core titanium sleeve and blades O ring 1AF3 Core O ring 1AG145 Sample outlet hole O ring 1AP135 Titanium sleeve Blade Blade Sample inlet hole 13 ...

Page 33: ...cover Make sure the O rings are free from scratches and apply a thin coat of vacuum grease on them The shaft D can be introduced 15 mm in the cover Put it in until it is held against Two O rings are used on the top and bottom ends of the shaft D 6 Sufficiently tighten the swageloknut until the clutch of the torque wrench functions It functions at 5 N m 51kgf cm Match mark Shaft D O ring 1AS5 Nut O...

Page 34: ...ntrifuge turn on the POWER switch and open the door To remove the door hole cover use the door hole adapter handle 2 Install the rotor gently on the spin shaft 3 Close the door Apply a thin coat of vacuum grease on the door seal O ring and mount it back in position O ring 15 ...

Page 35: ...tor is positioned approximately at the center of the center hole of the door adapter If the eccentricity is too great the door may have been set to the incorrect position Return to step 3 and close the door firmly 5 By means of the furnished screwdriver loosen 2 3 turns six setscrews for stopper A of door adapter Shaft D Screwdriver Stopper A 16 ...

Page 36: ...he door adapter Then turn about 60 to engage the stopper with the door adapter in such manner that the water passage faces the rear of the centrifuge 8 Position the bearing housing stopper B which prevents the bearing housing from turning in the gap between the bearing housing and door adapter a Connect to OIL IN e Hole for water passage b Connect to DRAIN IN d Connect to OIL OUT c Connect to WATE...

Page 37: ...rotor Inspect the assembled state of the bearing housing and rotor shaft and the bending of the shaft and correct If required 3 When the rotor attains a speed of more than 950 rpm turn on the VACUUM switch to vacuumize the rotor chamber 4 After 30 60 seconds cancel the ZONAL mode and stop the rotor 10 Using the screwdriver tighten the six stopper A setscrew loosened in 5 11 Insert the seal tube he...

Page 38: ...n removing the seal The top end of the seal is an important section which prevents the liquid from leaking If this end is damaged a defect such as a leakage of liquid will occur Take great care when handling this section 13 Connect tubes to the seal body assembly to make it ready for mounting Pressure gage Pump Tee Screw socket B Water connector Screw socket A Installing O rings on Seal Tube Outle...

Page 39: ... passage of the bearing housing This is necessary so the cooling water can flow smoothly for efficient cooling 15 Introduce the seal body assembly gently in the bearing housing and tighten by turning the screw socket A clockwise while pushing the screw socket B downward At this time take care not to move the bearing housing If it is dislocated shaft D will become eccentric Perform readjustment fro...

Page 40: ...l assembly to check that there is no leakage of cooling water 17 Turn the screw socket B of the seal body assembly to adjust its height to the position of the red line on the pipe The upper surface of manifold A should be positioned so that some of the red line is still visible If the red line is invisible even by tightening the screw socket B fully the screw socket A may not have been tightened s...

Page 41: ...gf cm2 This is not abnormal and can be corrected by filling with the overlay solution in the subsequent stage 3 After the density gradient solution etc are injected change the piping as illustrated and allow overlay solution from the central connector at a flow rate of about 30 ml min When overlay solution overflows from the central connector the density gradient solution has been injected complet...

Page 42: ...nconvenience cannot be eliminated by such measures decelerate the speed to 3 000 rpm and carry out the above steps to eliminate air Caution During high speed revolution check the vacuum degree and if it has dropped unusually or an anomaly has occurred stop operation immediately Poor vacuum may be caused by It is better that no bubbles etc are mixed in the sample 6 After the sample is injected flow...

Page 43: ...tion to the external wall of the rotor 24 At this time fill the tube to be injected with extrusion solution Use an extrusion solution the density of which is higher than the density gradient solution or cushion solution injected into the rotor 2 Send extrusion solution from the outer connector and push out the separated sample and density gradient solution from the central connector Thereafter sto...

Page 44: ...e swagelok nut Install the rotor on the Rotor base and remove the cover Pull out the core by means of the core handle Here recover pellets if any 5 As for the centrifuge clean the chamber interior close the door mount the door hole cover and turn off the power switch Although a small amount of oil leaks into the chamber this is normal 4 3 Capacity in rotor Fig 13 and 14 show the relationship betwe...

Page 45: ...mechanical pump Turning on the OIL switch operates the mechanical pump Lubricant is delivered from the OUT connector and returns to the OIL bottle through the IN connector The oil level in the OIL bottle is becoming the half of the bottle replenish The OIL switch also serves as a safety feature actuating switch If lubricant stops flowing with the OIL switch ON the TROUBLE lamp comes on it inform i...

Page 46: ...p and DRAIN bottle Turning on the DRAIN switch starts the vacuum pump which sucks and recovers to the DRAIN bottle waste oil discharged from the bearing housing Please check the waste oil quantity of the DRAIN bottle after operation If the reading is high throw away waste oil ...

Page 47: ...llowable speed 5 2 Precautions on spinning rotor Never spin the rotor at a speed over 3 000 rpm unless it is filled with the liquid If this is neglected the core will be damaged Never spin the rotor at a speed over 3 000 rpm unless the seal attachment is mounted If this neglected the shaft D will be damaged 5 3 Handling the shaft D Since the shaft D mounted on the rotor top is one of the important...

Page 48: ...reful not to damage the rotor 4 Clean the decal hole on the rotor by using alcohol 5 Remove the backing paper from the new over speed decal Place the over speed decal so that it snugly fits into the groove of the decal hole on the rotor Make sure the decal does not move n Caution The over speed decal plays an important role to control the rotor revolution speed The over speed decal must match the ...

Page 49: ...phate ammonium sulfate amyl acetate amyl alcohol borax bromine butyl alcohol butyl cellosolve butyl mercaptan benzoic acid boric acid butyric acid calcium chloride calcium hydroxide carbon bisulfide cesium chloride chloracetic acid chlorosulfonic acid citric acid copper chloride copper nitrate copper sulfate cyclohexane ethyl acetate ethyl chloride ferric chloride formic acid hydrobromic acid hydr...

Page 50: ...e 3 Disassemble and check Blocking is likely to occur in the seal body assembly Too much drainage 1 Transparent oil drains 2 White solution sample drains 1 The gap between shaft D and the bearing becomes too great and too much oil is components 2 Leaked from sealed surface See Table 6 Bubbles enter the sample line 1 The pressure rises 2 The sample stops flowing 1 If the pressure does not rise so m...

Page 51: ...injury or step between inner and outer rings Replace the seal Insertion recess for O ring Poor sealing due to injury in recess portion in contact with O ring 1 Poor sealing due to degradation or injury Replace the seal Bypassing O ring 1AP3 2 Poor sealing due to insufficient grease Apply grease 1 Poor sealing due to degradation or injury Replace the O ring Overlay solution line leaks Seal O ring 1...

Page 52: ...s and if e ither value reaches the figures the primary life shown in Table 8 decrease the maximum speed of the rotor by 10 If the operations or operating hours of rotor reach the figures in Table 8 after decreasing the maximum speed by 10 Secondary life don t use the rotor any more This specification is applied to the rotor body assembly Shaft D and the seal are consumable parts Table 8 Rotor life...

Page 53: ...alid for five years after delivery However the following guarantee is applied to the seal attachment assembly and lubricating unit n Keep Rotor Operation Log Regularly Rotor operation log is necessary for control of rotor life and guarantee against trouble Be sure to make an entry whenever operating the rotor and keep the log in good order If this procedure is neglected the rotor will not be guara...

Page 54: ...re it in a desiccator When a corrosive sample is handled immerse the rotor in warm diluted solution of neutral detergent at pH7 excluding one containing chlorine immediately after end of operation and rinse it properly with distilled water If foreign matter is present on the tapered opening at the rotor bottom the rotor might not fit the crown properly thereby breaking the rotor or spinning shaft ...

Page 55: ...nse them with distilled water dry and store them Maintenance of seals After use wash them with warm water dry and store them Take care not to scratch the contact surface of the seal Checkup of rotor Check the rotor for corrosion every 100 hr of operation Check particularly carefully the round portion of the rotor tapered opening at the rotor bottom marked in Fig 17 etc because the mechanical resis...

Page 56: ...igon tube and tee Wash them properly before storing them 9 3 The maintenance of lubricating unit ass y Check lubricating oil quantity in the OIL bottle and in the case below the half of the bottle replenish The capacity of the bottle is 500 ml Pour in about 400 ml Check a DRAIN bottle and throw away waste oil Incidentally the cap of the bottle fasten up sufficiently 9 4 Sterilization of rotor The ...

Page 57: ...cm K factor 7 500 10 000 12 500 15 000 17 500 20 000 22 500 25 000 27 500 30 000 32 000 4 712 8 378 13 090 18 849 25 656 33 510 42 411 52 359 63 355 75 398 85 786 5 152 9 159 14 312 20 609 28 051 36 638 46 369 57 246 69 268 82 435 93 792 5 586 9 930 15 516 22 343 30 411 39 721 50 271 62 063 75 097 89 371 101 685 765 431 276 191 141 108 85 69 57 48 42 ...

Page 58: ...45 484243 Blade 451153 Seal packing 8062222 O ring 1AS22 Rotor 51359 Overspeed decal 218343E Rotor body A ass y Door Seal attachment ass y 486138 O ring 1AP32 486137 O ring 1AG30 484281 Screw socket A 343929 Collar 484250 Seal 8062014 O ring 1AP14 8062003 O ring 1AP3 S408048A Seal tube head D ass y S218349A Bearing housing ass y 484301 O ring 1AG50 484300 O ring 1AG55 84520201 O ring for door S304...

Page 59: ... for door ID180 2 S999198 Instruction manual 1 11 2 2 Rotor body assembly accessories Sketch Part No Part name Q ty 218343E Rotor body A ass y 1 484253 Core setter ass y 1 343920 Holder base ass y 1 323663 Handle ass y 1 4692832 Torque wrench 1 484261 Seal extractor 1 484258 Seal tube head setter ass y 1 464912 Screw driver 1 216715 Rotor stand 1 ...

Page 60: ...5 2 477919 O ring for core 1AG145 ID145 2 8062003 O ring for core seal 1AP3 ID3 4 8062004 O ring for shaft 1AP4 ID4 2 8062205 O ring for shaft 1AS5 ID5 2 8062014 O ring for seal 1AP14 ID14 2 484250 Seal 2 320717 Tool box B 1 660557 Silicone grease 1 84810601 Lubricant for screw 1 999494 Logbook of rotor 1 51359 Overspeed decal 2 ...

Page 61: ...301 O ring for bearing housing 1AG50 ID 50 2 8062022 O ring for bearing housing 1AP22 ID 22 3 8062009 O ring for seal body ass y 1AP9 ID 9 2 8062010 O ring for seal body ass y 1AP10 ID 10 2 486137 O ring for seal body ass y 1AG30 ID 30 2 486138 O ring for seal body ass y 1AP32 ID 32 2 664370 Nalgon tubing 3 16x5 16x1 16 3m 662905 Tigon outer tube R3603 inner 1 8 x outer 1 4 for sample 7 5m 484286 ...

Page 62: ...embly accessories Sketch Part No Part name Q ty S101713A Lubricating unit 2 ass y 1 S202667A Unit base ass y X 1 S306920 Unit base 2 1 8065203 M4 x 8 screw 4 4754482 Nylon braided hose 3 5m 411421 Band 4 463152 Drive oil ass y 1 ...

Page 63: ...e NORYL O ring Nitryle rubber 1 380 g 12 2 USAGE 1 Mount the core titanium sleeve and blades Make sure the three types of O ring are free from scratches Apply a thin coat of vacuum grease on them before installation Put the blades on the pins on the titanium sleeve 2 Operation Prepare for sipping the TCF 32 rotor after the same manner of TCF 32 INSTRUCTION MANUAL Caution The volume of 940ML core i...

Page 64: ...hion solution Fig 19 Capacity 12 4 Determination of sample flow rate The separation characteristics with respect to the sample flow rate by this continuous flow rotor are given in Fig 20 Determine the flowing in rate of the sample to be separated referring to Fig 20 Fig 20 Separation characteristics Fig 18 Radius in rotor R3 R2 R1 Capacity ml I R2 I R3 I R1 Flow rate in 1 hr Sedimentation coeffici...

Page 65: ... following equation Do not spin the rotor beyond the allowable speed 12 6 COMPONENTS AND ACCESSORIES 12 6 1 Components 12 6 2 Accessories Sketch Part No Part name Q ty 487816 O ring for core 1AP100 ID100 2 487815 O ring for core 1AG110 ID110 2 8062003 O ring for core seal 1AP3 ID3 4 Allowable maximum speed Maximum speed x Maximum density of gradient solution g ml 1 2 S200911 Core 8062003 O ring 1A...

Page 66: ...y the service contract or requested by the user In cases where no service contract exists the preventive maintenance procedures must be performed by qualified service personnel selected at the discretion of the user preferably twice a year The inspection and cleaning procedures that should be done routinely by the user are found in Chapter 9 of the rotor instruction manual Check that the routine m...

Page 67: ...he water d Rotor Inspection 1 Corrosion of rotor To inspect the rotor properly refer to Chapter 9 of the rotor instruction manual 2 Rotor structural inspection Check the condition of the screw part of cover and rotor body the insertion condition to install cover in the rotor body and the insertion condition to install core into the rotor 3 Seal packing and metal ring Inspect and clean the seal pac...

Page 68: ... the seal assembly When WATER switch is turned on the lamp is turned on And the cooling water passes through the seal assembly properly 5 Condition of the DRAIN line A little transparent oil is sucked inside the drain tubing under the condition that TCF 32 system is rotating b Test Run 1 Check by the high speed rotation Rotate from 0 rpm to 32 000 rpm by centrifuge rotation noise and seal assembly...

Page 69: ...n of the DRAIN line n Rotor Inspection Corrosion of rotor Rotor structural inspection Seal packing and metal ring Injury of the core and the titanium sleeve Injury or wear on the seal surface Injury or wear on the bearing contact The O ring of the shaft D surface of the shaft D Functional Checks n Lubricating unit OIL Switch WATER switch DRAIN switch Flow condition of the OIL and the WATER Conditi...

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