Thermo Scientific Sorvall ST 8 Instruction Manual Download Page 9

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Preface

Sorvall ST 8 Centrifuge

Thermo Scientific

Symbols used on the centrifuge

Symbols used in the manual

Observe the information contained in the instruction manual to keep yourself and your environment
safe.

This symbol refers to general hazards. Follow the instructions in the instruction manual in
any case.

This symbol refers to biological hazards..

Observe the information contained in the instruction manual to keep yourself and your
environment safe.

This symbol refers to information on hazards, described within the manual.

This symbol refers to disconnect mains before transporting or servicing the centrifuge.

This Symbol refers to check, if the rotor is installed correct by lifting it slightly at the handle.
See

“Rotor Installation”

page 31

This symbol refers to general hazards.

CAUTION means that material damage could occur.

WARNING means that injuries or material damage or contamination could occur.

This symbol refers to biological hazards.

This symbol refers to electrical hazards.

Summary of Contents for Sorvall ST 8

Page 1: ...50138912 a February 2013 Thermo Scientific Sorvall ST 8 Centrifuge Instruction Manual Visit us online to register your warranty www thermoscientific com labwarranty ...

Page 2: ......

Page 3: ...Thermo Scientific Sorvall ST 8 Centrifuge Instruction Manual 50138912 a February 2013 ...

Page 4: ...ents Co Ltd No 297 Taishan Road New District Suzhou Jiangsu P R China Importer for Europe authorised representative Thermo Electron LED GmbH Robert Bosch Straße 1 D 63505 Langenselbold Germany The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersed...

Page 5: ... the following symbol Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union EU Member State and these companies should dispose of or recycle this product See www thermo com rohsweee for further information on Thermo Fisher Scientific s compliance with these Directives and the recyclers in your country ...

Page 6: ... 20 Before Use 21 Before Setting up 22 Location 22 Transporting the Centrifuge 23 Aligning the Centrifuge 23 Mains Connection 24 Storage 24 Shipping the Centrifuge 25 Control Panel 27 Control Panel 28 Keys 28 Operation 29 Switch on Centrifuge 30 Open Lid 30 Close Lid 30 Rotor Installation 31 Entering Parameters 32 Programs 34 Centrifugation 35 Short term Centrifugation 37 Removing the Rotor 37 Aer...

Page 7: ... Intervals 46 Cleaning 46 Disinfection 48 Decontamination 49 Autoclaving 50 Thermo Fisher Scientific Service 50 Disposal 51 Troubleshooting 53 Mechanical Emergency Door Release 54 Troubleshooting by Guide 55 When to Contact Customer Service 57 Chemical Compatibility Chart 59 Contact 65 ...

Page 8: ... your nearest Thermo Fisher Scientific representative Intended Use This centrifuge is a laboratory product used to separate substance mixtures of different densities This centrifuge can become an In vitro diagnostics device Directive 98 79 EC if used together with the hematocrit rotor and his accessories The hematocrit value is determined using the instructions written in the instruction manual of...

Page 9: ...instruction manual to keep yourself and your environment safe This symbol refers to information on hazards described within the manual This symbol refers to disconnect mains before transporting or servicing the centrifuge This Symbol refers to check if the rotor is installed correct by lifting it slightly at the handle See Rotor Installation on page 31 This symbol refers to general hazards CAUTION...

Page 10: ... work environment should guarantee the following WARNING Maintain a radius of at least 30 cm around the centrifuge Implementation of special measures which ensure that no one can approach the centrifuge for longer than absolutely necessary while it is running The power supply must be interrupted in case of an emergency Turn off the centrifuge at the main switch The mains plug must be freely access...

Page 11: ...nd or cracks Do not use rotors which show any signs of corrosion and or cracks Do not touch the mechanical components of the rotor and do not make any changes to the mechanical components Use only with rotors which have been properly installed Follow the instructions on the Thermo Scientific Auto Lock rotor exchange in section Rotor Installation on page 31 Use only with rotors which have been load...

Page 12: ... hazardous materials mind the Laboratory Biosafety Manual of the World Health Organization WHO When centrifuging microbiological samples from the Risk Group II according to the Laboratory Biosafety Manual of the World Health Organization WHO aerosol tight biological seals have to be used Look on the internet page of the World Health Organization www who int for the Laboratory Biosafety Manual For ...

Page 13: ...Preface 14 Sorvall ST 8 Centrifuge Thermo Scientific ...

Page 14: ...trifuge 15 1 Introduction and Description Contents Characteristics on page 16 Technical Data on page 17 Directives Standards and Guidelines on page 18 Functions and Features on page 19 Mains Supply on page 19 Rotor Selection on page 20 ...

Page 15: ...ntry mode These settings can be changed even while the centrifuge is running With the help of the PULSE key you can also centrifuge a sample for just a few seconds The Thermo Scientific Sorvall ST 8 Centrifuge is equipped with various safety features The housing and the interior consist of steel plate the rotor chamber of stainless steel while the front panel is made of high impact resistant plast...

Page 16: ...arly up to 50 relative Humidity at 40 C Permissible Ambient Temperature during Operation 2 C to 35 C Permissible Ambient Temperature during Storage and Shipping 10 C to 50 C Overvoltage Category II Pollution Degree 2 Heat Dissipation Ventilated 0 31 kWh h 1060 Btu h 1120 kJ h IP 20 Max Running Time 99 h 59 min 50 sec hold Max Speed nmax 16000 rpm depending on the rotor Min Speed nmin 300 rpm Max R...

Page 17: ...tive on Waste Electrical and Electronic Equipment 1907 2006 REACH Regulation on Registration Evaluation Authorization and Restriction of Chemicals including requirements for SVHC substances of very high concern EN 61010 1 2nd Edition EN 61010 2 020 2nd Edition EN 61010 2 101 2nd Edition EN 61326 1 Class B EN 61326 2 6 EN ISO 13485 USA Canada 230V 60Hz 120V 60Hz FDA Device Class 1 Product code JQC ...

Page 18: ...vanized steel chassis with armoured plating Chamber Stainless steel Drive Induction drive without carbon brushes Keys and display Easy to clean keypad and display surface Controls Microprocessor controlled Internal memory The most recent data is saved Functions RCF selection Acceleration braking profiles Standard and Soft Acceleration Deceleration Rotor recognition Automatic Electric Imbalance rec...

Page 19: ...our website at http www thermoscientific com Thermo Scientific Rotors Part No TX 150 swinging bucket rotor 75005701 TX 150 round buckets 75005702 TX 150 50mL conical buckets 75005703 TX100S clinical swinging bucket rotor with sealed carriers 75005704 TX100 clinical swinging bucket rotor with carriers 75005705 M10 microplate swinging bucket rotor 75005706 M10 with buckets 75005723 MT12 microtube sw...

Page 20: ... Sorvall ST 8 Centrifuge 21 2 Before Use Contents Before Setting up on page 22 Transporting the Centrifuge on page 23 Location on page 22 Aligning the Centrifuge on page 23 Mains Connection on page 24 Storage on page 24 ...

Page 21: ...ed indoors The set up location must fulfil the following requirements A safety zone of at least 30 cm must be maintained around the centrifuge People and hazardous substances must be kept out of the safety zone while centrifuging The supporting structure must be stable free of resonance and plane for example a laboratory bench The supporting structure must be suitable for horizontal setup of the c...

Page 22: ...a different location The supporting structure must be suitable for horizontal setup of the centrifuge WARNING Always lift the centrifuge on both sides Never lift the centrifuge by its front or back panel Always remove the rotor before moving the centrifuge Note Store the original centrifuge packaging Contact a shipping company for the transport or inform the customer service Always remove the roto...

Page 23: ... plate 5 Establish the connection to the power supply with the connecting cable Storage Before storing the centrifuge and the accessories it must be cleanend and if necessary desinfected and decontaminated Store the centrifuge in a clean dust free location Be sure to place the centrifuge on their centrifuge feet Avoid direct sunlight Main Power Switch Mains Plug WARNING When removing the centrifug...

Page 24: ...e please bear the following in mind The centrifuge must be clean and decontaminated The decontamination must be confirmed in a special form WARNING Before shipping the centrifuge and accessories you have to clean and if necessary disinfect or decontaminate the entire system In doubt contact the Thermo Fisher Scientific customer service ...

Page 25: ...2 Before Use Shipping the Centrifuge 26 Sorvall ST 8 Centrifuge Thermo Scientific ...

Page 26: ...Thermo Scientific Sorvall ST 8 Centrifuge 27 3 Control Panel Contents Control Panel on page 28 Keys on page 28 ...

Page 27: ...t the current settings Stop Press the STOP key to manually end the centrifugation run Open lid Press the OPEN key to activate the automatic release possible only when device is switched on Emergency release see Mechanical Emergency Door Release on page 54 Pulse Press the PULSE key to immediately start the centrifugation run and accelerate up to the maximal permissable end speed depending on the us...

Page 28: ...Centrifuge on page 30 Open Lid on page 30 Close Lid on page 30 Rotor Installation on page 31 Entering Parameters on page 32 Saving Programs on page 34 Centrifugation on page 35 Short term Centrifugation on page 37 Removing the Rotor on page 37 Aligning the Centrifuge on page 38 ...

Page 29: ...ime displays read 0 b When the centrifuge lid is open the following display shows The speed and time displays show the pre set values Open Lid 1 Press the OPEN key Close Lid Close the lid by pressing down on it lightly in the middle or on both sides of it WARNING Do not reach into the gap between the lid and the housing Use the emergency release only for malfunctions and power failures see Mechani...

Page 30: ...e centrifuge spindle The rotor clicks automatically into place 2 Check if the rotor is properly installed by lifting it slightly on the handle If the rotor can be pulled up then it must be reclamped to the centrifuge spindle CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge CAUTION Do not force the rotor onto the centrifuge spindle If the rotor is ve...

Page 31: ...TION DECELERATION key to set a profile The LEDs show the chosen settings The last profile is saved if you restart the centrifuge Be sure to check all sealings before starting any aeorsol tight applications See the information in the rotor instruction manual Settings Description OFF No Acceleration and Decelaration Standard SOFT ACC Acceleration Soft SOFT DEC Decelaration Soft SOFT ACC DEC Accelera...

Page 32: ...f an extremely low RCF value has been selected it will be corrected automatically if the resulting speed is less than 300 rpm Explanation of RCF Value The relative centrifugal force is given as a multiple of the force of gravity g It is a unitless numerical value which is used to compare the separation or sedimentation capacity of various devices since it is independent of the type of device Only ...

Page 33: ...us operation the centrifuge will continue running until you stop it manually Programs The Thermo Scientific SL 8 Centrifuge is able to save up to 4 programs It is only possible to save a program if the centrifuge is not running Saving Programs 1 Modify the speed and running time 2 Press the program key for 4 seconds you want to save the program with 3 Enter a name for the program There is place fo...

Page 34: ...ntrol panel The centrifuge accelerates to the pre set speed with the time display active If the speed setting is higher than the maximum permissible speed or RCF value for the particular rotor then the display will show the message Limit max permitted value in rcf or xg once the centrifuge has been started Within 10 seconds you can apply this value by pressing the START key again and the centrifug...

Page 35: ...e limit expires As soon as the speed drops to zero the message RUN COMPLETED will appear in the display By pressing the OPEN key you can open the lid and remove the centrifuged samples You can also stop the centrifuging program manually at any time by pressing the STOP key Continuous operation If you selected continuous operation see Continuous operation on page 34 you will have to stop the centri...

Page 36: ...n Check carefully whether you have to maintain a certain speed for your application During the acceleration process time is counted forwards in seconds The reading stays displayed until the centrifuge lid is opened Removing the Rotor To remove the rotor proceed as follows 1 Open the centrifuge lid 2 Grab the rotor handle and press against the green Auto Lock button At the same time pull the rotor ...

Page 37: ...the samples Figure 4 Auto Lock lid for aerosol tight rotors Aligning the Centrifuge To turn off the centrifuge push the mains switch to 0 CAUTION Rotors supplied with a lid for aerosol tight applications come with a mandrel which belongs to the Auto Lock Be sure not to place the lid onto this mandrel to prevent it from being damaged WARNING Mind the Auto Lock mandrel inside the lid Do not touch th...

Page 38: ... 39 5 System Menu To get into the system menu press and hold any button when turning on the centrifuge until the system menu shows up in the display Use the ARROW keys below the time selection in order to navigate within the system menu points ...

Page 39: ...5 System Menu 40 Sorvall ST 8 Centrifuge Thermo Scientific English Nederlands Росский Francais Español Italiano Deutsch 0602 V01 4758 V05 Software and NVRAM identification exam ple values ...

Page 40: ...SPEED in order to navigate through the system menu Press the START key to save this edit and quit the system menu Press the STOP key to quit the system menu End of run beep Use the ARROW keys below TIME until it says YES in the display The centrifuge beeps after the run Otherwise use the ARROW keys below TIME until it says NO Use the ARROW keys below SPEED in order to navigate through the system m...

Page 41: ...after the run Otherwise use the ARROW keys below TIME until it says NO Use the ARROW keys below the speed display in order to navigate through the system menu Press the START key to save this edit and quit the system menu Press the STOP key to quit the system menu Door auto open Use the ARROW keys below TIME until it says YES in the display The centrifuge opens the door automatically after the run...

Page 42: ...it the system menu NVRAM ID Here you find the current software version Use the ARROW keys below SPEED in order to navigate through the system menu Press the STOP key to quit the system menu Cycle count Here you fine the current numbers of cycles Use the ARROW keys below SPEED in order to navigate through the system menu Press the START key to save this edit and quit the system menu Press the STOP ...

Page 43: ...5 System Menu Description 44 Sorvall ST 8 Centrifuge Thermo Scientific ...

Page 44: ...vall ST 8 Centrifuge 45 6 Maintenance and Care Contents Cleaning Intervals on page 46 Cleaning on page 46 Disinfection on page 48 Decontamination on page 49 Autoclaving on page 50 Thermo Fisher Scientific Service on page 50 ...

Page 45: ...If drying boxes are used the temperature must never exceed 50 C since higher temperatures could damage the material and shorten the lifetime of the parts Use only disinfectants with a pH of 6 8 Dry aluminum parts off with a soft cloth After cleaning treat the entire surface of aluminum parts with corrosion protection oil part no 70009824 Also treat the cavities with oil Store the aluminum parts at...

Page 46: ...warm air cabinet at a maximum temperature of 50 C After cleaning treat the entire surface of aluminum parts with corrosion protection oil part no 70009824 Also treat the cavities with oil Tread the bolt of the swing out rotor with bolt grease part no 75003786 CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufa...

Page 47: ...Rinse the rotor and accessories thoroughly with water 9 Dispose of the disinfectant according to the applicable guidelines 10 Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 C After cleaning treat the entire surface of aluminum parts with corrosion protection oil part no 70009824 Also treat the cavities with oil Tread the bo...

Page 48: ...ly with water 9 Dispose of the decontamination solution according to the applicable guidelines 10 Dry all of the rotors and accessorie after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 C After cleaning treat the entire surface of aluminum parts with corrosion protection oil part no 7000 9824 Also treat the cavities with oil Tread the bolt of the swing out rotor wi...

Page 49: ...hnicians check the following the electrical equipment the suitability of the set up site the lid lock and the safety system the rotor the fixation of the rotor and the drive shaft the protective casing Thermo Fisher Scientific offers inspection and service contracts for this work Any necessary repairs are performed for free during the warranty period and afterwards for a charge This is only valid ...

Page 50: ...ted by the European Union s Waste Electrical Electronic Equipment WEEE Directive 2002 96 EC See WEEE Compliance on page 5 Mind the information on transport and shipping See Transporting the Centrifuge on page 23 and Shipping the Centrifuge on page 25 for more information WARNING When removing the centrifuge and accessories from use for disposal you have to clean and if necessary disinfect or decon...

Page 51: ...7 Disposal 52 Sorvall ST 8 Centrifuge Thermo Scientific ...

Page 52: ...Thermo Scientific Sorvall ST 8 Centrifuge 53 8 Troubleshooting Contents Mechanical Emergency Door Release on page 54 Troubleshooting by Guide on page 55 When to Contact Customer Service on page 57 ...

Page 53: ...ual Proceed as follows 1 Make sure the rotor has stopped view port in the lid 2 Pull out the power supply plug 3 On the right side of the housing is one white plastic plug which you can pry out of the side plate with a screwdriver Pull the release cord attached to it to trigger the mechanical door release The door will open and the samples can be removed Figure 5 Emergency Door Release 4 Push the ...

Page 54: ...uge is accessible Be sure that the room temperature is within the limits Let the centrifuge cool down for 15 minutes If the error message appears again inform the customer service E 017 E 020 E 021 E 022 E 023 E 078 E 079 E 080 E 081 Read Manual Wait until the rotor has stopped Check if the rotor is qualified for the Sorvall ST 8 Centrifuge check Rotor Selection on page 20 Check if the bottom of t...

Page 55: ...wer Failure Check the power supply of the centrifuge Make sure not to operate too many devices at one power source Let the centrifuge cool down for 15 minutes If the error message appears again inform the customer service E 098 Imbalance Load Check the load placed in the rotor Check that the rotor cross bolts are greased well Restart the centrifuge If the error message appears again inform the cus...

Page 56: ... the serial no of your centrifuge This information can be found on the back near the inlet for the power supply cable In addition the customer service also needs the Software ID and the NVRAM ID Both are available in the system menu For a desription how to get there see Enter system menu on page 41 You will find a description on how to find the Software ID on page 43 and for the NVRAM ID on page 4...

Page 57: ...8 Troubleshooting When to Contact Customer Service 58 Sorvall ST 8 Centrifuge Thermo Scientific ...

Page 58: ... S M S S Aluminum Chloride U U S S S S U S S S S M S S S S S S S S S M U U S S Formic Acid 100 S M U U U S M U U S S U S U S U Ammonium Acetate S S U S S S S S S S S S S U S S S S S S S S S S Ammonium Carbonate M S U S S S S S S S S S S S U U S S S S S S M S S S Ammonium Hydroxide 10 U U S U S S M S S S S S S U M S S S S S S S S S M S Ammonium Hydroxide 28 U U S U S U M S S S S S U S U M S S S S S...

Page 59: ... S U S M U U U M M U S S U U Diethylpyrocarbonate S S U S S S S S U S U S U S S S M S S S S S S Dimethylsulfoxide S S U U S S S S U S S U S U U S S U U S S S S U U Dioxane M S U U S S M M S U U S U M U U M M M U S S S S U U Ferric Chloride U U S M S M S S S S M U S S Acetic Acid Glacial S S U U S S U M S U S U U U U U M S U M U S U U S U Acetic Acid 5 S S M S S S M S S S S S M S S S S S S S M S S ...

Page 60: ...xide 5 U U S S S S M S S S S S U S S S S S S S M U M S U Potassium Hydroxide conc U U M U M M S S U M U U U S M M U U U U Potassium Permanganate S S S S S S S S S U S S S M S M S U S S M S U S Calcium Chloride M U S S S S S S S S S S S S M S S S S S S S M S S S Calcium Hypochlorite M U S M M S M S S M S S S S M S M U S S Kerosene S S S S S S U S M U S U M M S M M M S S U S S U S Sodium Chloride 10...

Page 61: ... S S S Sodium Sulfide S S S S S S S U U S S S M S Sodium Sulfite S S S S S S S M S S S S S S M S S S S S S S S S S Nickel Salts U S S S S S S S S S S S S S S S S S S M S S S Oils Petroleum S S S S U S S S S U U M S M U U S S S U S S S S Oils Other S S S M S S S S U S S S S U S S S S S S M S Oleic Acid S U S S S U U S U S S M S S S S S S S S S M U S M M Oxalic Acid U U M S S S U S S S S S U S U S S...

Page 62: ...cid S S S M S S S S S S S S S S S S S M M S S S Tetrahydrofuran S S U U S U U M S U U S U U U M U U U U S U S S U U Toluene S S U U S S M U S U U S U U U S U M U U U S U S U U M Trichloroacetic Acid U U U S S U M S U S U U S M M S S U U S U U U M U Trichloroethane S U M U U S U U U U U U U U U S U S S Trichloroethylene U U U U S U U U U U U U U U S U U S Trisodium Phosphate S M S S S S S S S Tris ...

Page 63: ... NORYL NYLON PET POLYCLEAR CLEARCRIMP CCCLEARCRIMP POLYALLOMER POLYCARBONATE POLYESTER GLASS THERMOSET POLYTHERMIDE POLYRTHYLENE POLYPROPYLENE POLYSULFONE POLYVINYL CHLORIDE RULON A TEFLON SILICONE RUBBER STAINLESS STEEL TITANIUM TYGON VITON Key S Satisfactory M M Moderate attack may be satisfactory for use in centrifuge depending on length of exposure speed involved etc suggest testing under actu...

Page 64: ... 33 2 2803 2180 Italy 39 02 95059 552 Netherlands 31 76 579 55 55 Nordic Baltic Countries CIS 358 9 329 10200 Russia 7 812 703 42 15 Spain Portugal 34 93 223 09 18 Switzerland 41 44 454 12 22 UK Ireland 44 870 609 9203 China 86 21 6865 4588 86 10 8419 3588 India 91 22 6716 2200 Japan 81 45 453 9220 Other Asian Countries 852 2885 4613 Australia 61 39757 4300 New Zealand 64 9 980 6700 Countries not ...

Page 65: ...10 Contact 66 Sorvall ST 8 Centrifuge Thermo Scientific ...

Page 66: ...e 55 Features 19 I Imbalance indicator 35 K Keys 28 Kundendienst 50 57 L Lid Opening 30 Location 22 M Mains Connection 24 Mains Supply 24 Maintenance 45 Maximum Loading 35 O Operation 29 P Preface 9 Preselecting Speed 33 R Removing the Rotor 37 Rotor Installation 31 Rotor Selection 20 Running time preselection 34 S Safety features 16 Safety zone 22 Shipping the Centrifuge 25 Short term Centrifugat...

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