3
Applications
Measure Custom
Thermo Scientific
NanoDrop One
C
with NanoDropQC Software User Guide
37
Custom method settings
These settings are available for creating custom methods.
Setting
Available Options
Result name
Enter descriptive name for calculated concentration result (for example, “Polymer A
analysis”) and use adjacent drop down list to select appropriate unit. Result name
appears as column heading for reported concentration value.
Measurement range
Select spectral range in which method will acquire data.
Available options:
• Ultra-violet only (190 nm - 350 nm)
• Visible only (350 nm - 850 nm)
• Ultra-violet and visible (190 nm - 850 nm)
• Custom (specify starting and ending point in nanometers)
Notes
:
• If a
Analysis wavelength correction
are used, make sure
your selected spectral range includes your specified baseline correction and/or
analysis correction wavelength.
• For micro-volume absorbance measurements and measurements taken with non-
standard (other than 10 mm) cuvettes, the spectra are normalized to a 10 mm
pathlength equivalent.
Analysis wavelength
correction
Use this option to specify absorbance correction at analysis wavelength only. Available
options:
•
None.
No correction at analysis wavelength.
•
Single point
. Enter wavelength for analysis correction. (Absorbance value at
specified analysis correction wavelength is subtracted from absorbance value at
analysis wavelength. Corrected value is used to calculate sample concentration.)
•
Sloping baseline
. Enter two wavelengths that define sloping baseline for analysis
correction. (Absorbance value of sloping baseline at analysis wavelength is
subtracted from absorbance value at analysis wavelength. Corrected value is used
to calculate sample concentration.)
