3
Applications
Measure UV-Vis
Thermo Scientific
NanoDrop One
C
with NanoDropQC Software User Guide
13
Best practices for UV-Vis measurements
• Ensure the sample absorbance is within the instrument’s
.
• Blank with the same buffer solution used to re-suspend the analyte of interest. The
blanking solution should be a similar pH and ionic strength as the analyte solution.
• Run a
to assess the absorbance contribution of your buffer solution. If the
buffer exhibits strong absorbance at or near an analysis wavelength, you may need to
choose a different buffer or application. See
Choosing and Measuring a Blank
information.
• For micro-volume measurements:
–
Ensure pedestal surfaces are properly
and
–
Ensure samples are homogeneous before taking a measurement. Avoid introducing
bubbles when mixing and pipetting.
–
Follow
best practices for micro-volume measurements.
–
Use a 1-2 µL sample volume. See
for more
information.
3. Pipette 1–2 µL blanking solution onto the lower pedestal and lower the arm, or insert
the blanking cuvette into the cuvette holder.
Tip
: If using a cuvette, make sure to
with the instrument
light path.
4. Tap
Blank
and wait for the measurement to complete.
Tip
: If
is On, the blank measurement starts automatically after you lower
the arm. (This option is not available for cuvette measurements.)
5. Lift the arm and clean both pedestals with a new laboratory wipe, or remove the
blanking cuvette.
6. Pipette 1-2 µL sample solution onto the pedestal and lower the arm, or insert the
sample cuvette into the cuvette holder.
7. Start the sample measurement:
–
Pedestal: If
is On, lower arm; if Auto-Measure is off, lower arm and
tap
Measure
.
–
Cuvette: Tap
Measure.
When the sample measurement is completed, the spectrum and reported values are
displayed (see the next section).
8. When you are finished measuring samples, tap
End Experiment
.
9. Lift the arm and clean both pedestals with a new wipe, or remove the sample cuvette.
