Thermo Scientific NanoDrop OneC, User Manual, Page: 63 / 144

4
Learning Center
Measure a Micro-Volume Sample
Thermo Scientific NanoDrop One
C
with NanoDropQC Software User Guide 59
Best practices for micro-volume measurements
Cleaning pedestals for daily operation
• Before first measurement, clean both
pedestals with a new laboratory wipe.
• Run a blanking cycle to verify pedestals
are clean.
• After each measurement, clean both
pedestals with new wipe to prevent
carryover.
• After each set of measurements, clean
pedestals with DI H2O (see Clean
pedestals between users )
• Recondition pedestals periodically to
maintain their hydrophobic property.
Pipetting Samples
• Use recommended sample volumes to
ensure proper liquid column
formation.
• Use calibrated precision pipettor (0–
2 µL volume range) with well-fitting,
low-retention precision tips to apply
sample material to instrument for
measurement.
If using low accuracy (0-10 µL)
pipettor, use 2 µL sample volumes.
• Use new tip for each blank and sample
aliquot.
• Use new aliquot of sample for each
measurement.
• If solvents are used, make sure they are
compatible with the pedestals. (see
“Compatible Solvents” in
Hazardous
Materials ).