34
Maintenance
F.1.2 Single Channel Cleaning and Autoclaving
General Cleaning:
Carefully wipe down all disassembled plastic and metal parts with methyl
alcohol or 70% isopropyl alcohol.
Decontamination/Autoclaving:
Decontaminate all disassembled parts by wiping them with a bleach solution
(5% sodium hypochlorite) or by autoclaving the parts at 121 °C for 20 minutes.
Re-lubrication:
Lightly coat piston with O-ring lubricant and replace or re-install O-ring on the
piston, as described in reassembly procedure.
Note:
Do not clean the O-ring. Replace the O-ring after decontamination
or autoclaving (O-ring and O-ring lubrication is available from Thermo Fisher
Scientific).
F.1.3 Pipette Reassembly
After cleaning or decontaminating the disassembled parts, the pipette can be
reassembled (
refer to Figure F.1.1c for parts identification and reassembly
order
).
1.
Slide the 1/2” diameter spring, O-ring support, and O-ring over the piston
and motor screw. (
see Figure F.1.3a
).
2.
Slide the cylinder over the O-ring and piston assembly and screw into the
pipette body so that it is finger tight. Do not over-tighten.
3.
Assemble collet, 1.0” diameter
ejector spring, and ejector sleeve.
Note:
Ensure the tab on the
ejector sleeve is aligned with the
notch in the collet.
Figure F.1.3a: O-ring and piston assembly