Thermo Scientific Matrix 1020 User Manual Download Page 26

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Programming and Operation

C.3 Operating Procedures

After programming the pipette, the display will show the first program
step, with the “Run” prompt displayed in the lower left corner:

The following steps describe a basic program for pipetting reagent into a
microtiter plate. The pipette is programmed as follows:

[Fill]

Vol

(250) [Enter]

Note:

The 10 µl

Primer Dispense
reduces the
percentage of error
in incremental
dispensing (see
section D.3
Recommendations for
Accurate Pipetting)

[Disp]

Vol

(10) [Enter]

[Disp]

Vol

(30) [Enter] [Enter]

1. Attach pipette tips.

When ready to begin pipetting, attach the tips to the

pipette.

Note:

When applying tips, avoid ‘banging’ or applying excessive force to the

tip(s), as this stretches the tip seal and will affect volume. Use Matrix tips which

have been specifically designed to match the tip fittings on Matrix pipette.

2. Aspirate liquid.

Immerse the pipette tips into the liquid to be aspirated,

then press the trigger to aspirate 250 µl. Submerge the tips approximately
1/4” below the surface of the liquid.

3. Dispense primer volume.

Discard the 10 µl primer volume back to the

source vessel or into a waste receptacle.

Figure C.3:

Run Prompt

Summary of Contents for Matrix 1020

Page 1: ...Thermo Scientific Matrix Electronic Pipette User Manual ...

Page 2: ...Manual N09238 Thermo Scientific Matrix Electronic Pipette User Manual ...

Page 3: ...Pipette Specifications 11 B 6 Hazards and Precautions 12 C PROGRAMMING AND OPERATION 13 C 1 Programming the Electronic Pipette 13 C 1 1 Open Programming Mode or Scratch Pad P 0 13 C 1 2 Entering a Program in Open Programming Mode 13 C 1 3 Storing a Program 16 C 1 4 Program Recall 17 C 1 5 Adjusting Pipetting Speed 17 C 1 6 Reviewing the Program 17 C 2 Programming Examples 18 C 2 1 Sample Transfers...

Page 4: ...channel Maintenance 35 F 2 1 Multichannel Disassembly 35 F 2 2 Multichannel Cleaning and Autoclaving 38 F 2 3 Multichannel Maintenance and Reassembly 38 F 3 Installing Replacing Batteries 44 F 3 1 Installing Replacing NiMH Batteries 44 F 3 2 Installing Replacing Lithium Ion Battery 16 Ch 48 F 4 Charging Recharging Batteries 50 F 4 1 Charging Recharging NiMH Batteries 50 F 4 2 Charging Recharging L...

Page 5: ......

Page 6: ...ociated with manual pipette use Laboratory results will become more standardized while less time is expended on tedious bench procedures Before You Begin Before using the Matrix electronic pipette verify the contents of the package The package should contain the following items 1 Electronic Pipette 1 Operator s Manual 2 Rechargeable NiMH Batteries or 1 Lithium Ion Battery 16 Channels 1 AC Power Tr...

Page 7: ...in lab calibration verification using distilled water The Matrix electronic pipette is lightweight and cordless utilizing rechargeable batteries that can be used during recharge with the exception of single channel pipettes The pipette also has an automatic shut off feature to extend battery life The electronic pipette uses integrated circuit technology to store programming information It will hol...

Page 8: ...safety cabinet A 2 Thermo Scientific Matrix Multichannel Electronic Pipettes The multichannel electronic pipettes are available in a number of configurations from 8 to 16 channnels An expandable EXP pipette is also available in 6 channel and custom configurations Models range in volume from 0 5 µl to 1250 µl Each pipette is color coded for easy volume identification see section B 5 Pipete Specific...

Page 9: ...4 Pipette Overview B PIPETTE OVERVIEW B 1 Components Figure B 1b Multichannel Components Figure B 1a Single Channel Components ...

Page 10: ... step is executed by a quick press and release of the trigger Repeat pipetting is executed by pressing and holding the trigger Note At the end of any dispense the pipette tips should be removed from the fluid before releasing the trigger to prevent aspiration of fluid Ejector Trigger The ejector trigger is pressed to eject the tips from the pipette Tip Fittings The tip fittings form an airtight se...

Page 11: ...he RUN mode Note If the pipette is being programmed for the first time the first step will be the fill operation with the default fill volume See Section C 1 Programming the pipette for the default settings Prog The Program key on the Matrix electronic pipette enables you to store up to five separate programs in memory for future recall Each program can hold up to 40 steps Programs are stored in r...

Page 12: ... end of a pipetting sequence the pipette emits a triple beep tone and returns to the beginning of the program The pipette remains in RUN mode until you program a new pipetting sequence B 3 2 Programming Keys Fill Disp Mix The Fill Dispense and Mix keys program the pipetting operations By pressing the fill or mix key you can enter the programming mode of the electronic pipette The Run prompt disapp...

Page 13: ...olume key can also be used to change pipetting speeds within a stored program or during the execution of a program See Section C 1 5 Adjusting Pipetting Speed Enter The Enter key stores the program in memory After each step is programmed the Enter key is pressed once to accept the step After the final step is programmed the Enter key is pressed twice to denote the end of the program The pipette ve...

Page 14: ...l programming step attempted see Section E Troubleshooting Triple beep tone end of pipetting sequence end of programming sequence There are three options to choose from in setting the beep tone ON1 sounds all tones at the appropriate times listed above ON2 uses only double and triple beep tones OFF turns off all tones except for the tone to signify an illegal programming step Press the Special Fun...

Page 15: ...se step see Section C 5 Operating Procedures CAL The calibration of the pipette is factory set for distilled water at room temperature If you are pipetting fluids with significantly different specific gravities or temperatures use this setting to recalibrate the pipette See Section D Calibration The current speed setting appears at the bottom of the display These settings can be selected and chang...

Page 16: ...25 0 µl Yellow 2 0 1 0 µl 1 0 0 6 µl 2002 250 0 µl 8 Ch 5 0 250 0 µl Blue 2 0 1 5 µl 0 7 1 0 µl 2022 250 0 µl 8 Ch EXP 5 250 µl Blue 2 0 1 5 µl 0 7 1 0 µl 2004 1250 µl 8 Ch 15 1250 µl Green 1 5 6 0 µl 0 6 3 0 µl 2024 1250 µl 8 Ch EXP 15 1250 µl Green 1 5 6 0 µl 0 6 3 0 µl 2019 12 50 µl 12 Ch 0 50 12 50 µl Red 2 5 0 15 µl 2 0 0 15 µl 2229 12 50 µl 12 Ch EXP 0 50 12 50 µl Red 2 5 0 15 µl 2 0 0 15 µl...

Page 17: ...e power transformer supplied by Thermo Fisher Scientific Use of an incompatible power transformer can damage the pipette CAUTION Always turn off the power and unplug the power transformer before cleaning the exterior Fluid seepage can damage internal components CAUTION Do not immerse the unit in liquid Fluid seepage can damage internal components CAUTION Do not autoclave the entire unit Extreme he...

Page 18: ... program section of the display refer to the pipette display on page 10 This feature enables quick changes to pipetting protocols without affecting previously stored programs To use the scratch pad mode From the start of any program simply begin entering a new protocol selecting a pipetting operation as described below This new protocol can now be used and will remain active until a stored program...

Page 19: ...he default volume for the pipette if it is being programmed for the first time Fill The Fill key programs the pipettor to aspirate a volume of liquid A single fill step can be programmed for fluid transfers or a combination of fill steps can be programmed for dilutions or supernatant collection Dispense The Disp key programs the pipette to dispense a volume of liquid The dispense volume can either...

Page 20: ...peated useful for serial dilutions unless followed by another program step To end the mix operation press the Purge key then press the trigger Purge The Purge key programs the pipette to purge the fluid This step can be entered as a program step or it can be pressed any time while pipetting to terminate the program and dispense the remaining liquid Note If the program ends at a volume greater than...

Page 21: ...eady to be run Go to Section C 1 6 for Reviewing the Program or Section C 3 for Operating Procedures C 1 3 Storing a Program To create and store programs in the pipette 1 Select the program number by pressing the Prog key then press the Vol key until the desired program number P1 P5 is displayed 2 Press and hold the Prog key while simultaneously pressing the Special Function key to enter the progr...

Page 22: ...ing the execution of a program Press the Vol key to select the speed for each pipetting step Fill Dispense or Mix only If purge is programmed as a step its speed can also be programmed Note Purge must be changed in the program This newly set speed will remain active with each function even if the pipette is turned off Speed settings within any of the five stored programs will also remain in memory...

Page 23: ...s of different pipetting sequences are described below and are ideal for use as training exercises Press the keys shown in brackets then press the Vol key until the volume in parentheses is displayed At the end of the program a triple beep tone is sounded see section D 3 Recommendations for Accurate Pipetting for suggestions for better precision and accuracy C 2 1 Sample Transfers Example Fill and...

Page 24: ...ecommendations for Accurate Pipetting for further explanation C 2 3 Serial Dilutions Example Transfer 100 µl to the first column of a plate previously filled with reagent Mix 200 µl for 2 cycles Transfer 100 µl to the second column and repeat the mix step Follow this procedure for the rest of the plate Fill Vol t s 100 Enter Mix Vol t s 200 Mix Vol t s 2 Enter Enter Enter That s the entire program...

Page 25: ...ally by the sample volume 10 µl The purge step will dispense the total volume C 2 5 Supernatant Collection Example Aspirate 100 µl from 8 rows of a Microtiter plate and discard the total volume Fill Vol t s 100 Enter Fill Enter Fill Enter Fill Enter Fill Enter Fill Enter Fill Enter Fill Enter Purge Enter Enter This program is performed with the 850 µl handle All 100 µl fill volumes are collected i...

Page 26: ...Disp Vol t s 10 Enter Disp Vol t s 30 Enter Enter 1 Attach pipette tips When ready to begin pipetting attach the tips to the pipette Note When applying tips avoid banging or applying excessive force to the tip s as this stretches the tip seal and will affect volume Use Matrix tips which have been specifically designed to match the tip fittings on Matrix pipette 2 Aspirate liquid Immerse the pipett...

Page 27: ... as a paced dispense Instead of pressing the trigger for each dispense step press and hold the trigger to automatically dispense each volume Immediately after each dispense step the pipette emits a single beep tone if the beep tone is on prompting you to prepare for the next dispense step To stop a paced dispense release the trigger before the end of the last dispense step Note For paced dispensin...

Page 28: ...me Measurement To measure the actual value of the programmed volume 1 Fill the programmed volume Vp then dispense the entire volume into a container on the analytical balance 2 Read the weight on the analytical balance Repeat this procedure ten times Note Use a clean and dry pipette tip with each pipetting cycle When filling the liquid immerse the pipette tip approximately 1 4 inch approx 0 6 cm b...

Page 29: ...n average value for all pipettes of its class This value may differ from the factory optimized calibration step documented on the Pipette Calibration Report Please use the step listed there for best results If you cannot locate your documentation that lists the factory calibration step contact technical service at 800 345 0206 and have your pipette s serial number ready D 2 2 EASY Cal Mode Electro...

Page 30: ...ter the EASY CAL mode to quickly re calibrate the pipette 3 Enter the EASY CAL mode by pressing the Special Function key using a pipette tip until EASY flashes then press the Enter key to display the calibration point 4 Use the Vol key to adjust the displayed calibration point until it matches the average weight obtained in step 2 Store this number by pressing the Enter key The new calibration set...

Page 31: ...isplay CALC pressing the Vol key again will return you to the EASY mode 5 Press the Enter key to display the calibration point 6 Press the Vol key to enter the Display Volume Dv calculated above in step 2 7 Press the Enter key to set the calibration point The display shows CAL then SET to confirm the new calibration point and returns to the current pipetting program Example Calibrate a 1250 µl 8 c...

Page 32: ...tion step documented on the Pipette Calibration Report certificate enclosed in this pipette s documentation D 3 Recommendations for Accurate Pipetting Matrix Electronic pipettes are factory calibrated to deliver accurate volumes with distilled water at 21 C and 50 humidity By using the calibration procedures described above you can pipette liquids of different specific gravity with a high degree o...

Page 33: ... Pause consistently It takes a moment for the liquid in the tip to finish moving Pause with the tip in the liquid for about one second after aspirating the sample to allow the liquid to finish moviing into the pipette tip after the pipette stops Otherwise the volume will be lower than it should Aspirate slowly dispense quickly As a general rule pipette liquids especially viscous liquids at a slowe...

Page 34: ...NiMH Ensure that the battery spacer is in place for all models that use Nickel Metal Hydride see section F 3 1 Double beep tone occurs Probable Cause Recommended Solution An illegal programming step is being entered such as programming two fills that exceed the maximum fill volume Recheck the programming sequence Ensure you are not attempting to fill above the maximum fill volume of the pipette or...

Page 35: ...O ring See Section E 2 2 Pipette Cleaning and Autoclaving Relubrication step and Section E 2 3 Pipette Reassembly Cylinder was not screwed tightly into the pipette body single channel only Securely screw the cylinder into the pipette body finger tightened Tubing was disconnected from tip fitting or cylinder Disassemble the pipette and reattach the tube If tube fits poorly it may have stretched and...

Page 36: ...ing internal components is required Thermo Fisher Scientific provides the following Preventive Maintenance Autoclave Kit Preventive Maintenance Autoclave Kit Replacement cross tubes O ring lubricant Wooden applicator O rings Item Description Item Description 9809 12 5µl 6 8 12 channel 9804 850µl 12 channel 9801 125µl 6 8 12 channel 9805 1250µl 6 8 channel 9802 250µl 6 8 12 channel CAUTION DO NOT A...

Page 37: ...ee Figure F 1 1a 2 Remove the collet and ejector sleeve by sliding both pieces down over the cylinder see Figure F 1 1b For reassembly later note the position of the large spring in the collet 3 Unscrew cylinder counter clockwise and remove it from the pipette body Note Check for the presence of the O ring on the piston If it is not on the piston look inside the cylinder and remove the O ring 4 Re...

Page 38: ...33 Maintenance Figure F 1 1c Pipette Tip Assembly ...

Page 39: ...ce the O ring after decontamination or autoclaving O ring and O ring lubrication is available from Thermo Fisher Scientific F 1 3 Pipette Reassembly After cleaning or decontaminating the disassembled parts the pipette can be reassembled refer to Figure F 1 1c for parts identification and reassembly order 1 Slide the 1 2 diameter spring O ring support and O ring over the piston and motor screw see ...

Page 40: ... Figure F 1 3b 6 Test the ejector function to ensure that the ejector sleeve moves down when the ejector trigger is pressed F 2 Multichannel Electronic Pipette Maintenance F 2 1 Multichannel Electronic Pipette Disassembly 1 Remove the color ring by gently prying loose one of the edges near the handle trigger 2 Use a Phillips screwdriver to remove all three housing screws 3 Carefully remove the pla...

Page 41: ...d remove comb first then trigger 5 Remove the piston cylinder assembly by pulling the motor screw down from the motor shaft coupling and out of the main housing Remove the manifold assembly by firmly lifting it out of the main housing support ribs see Figure F 2 1c Figure F 2 1c Removing Piston Cylinder and Manifold Assemblies Figure F 2 1b Removing Ejector Trigger and Ejector Comb ...

Page 42: ...box 18 Batteries 5 Driver board 19 Main housing 6 Motor 20 Trigger hinge pin 7 Color ring 21 Handle trigger 8 Motor coupling 22 Handle trigger spring 9 Motor screw 23 Ejector trigger 10 Piston backplate 24 Piston 11 Photosensor 25 O ring 12 Cylinder 26 Cross tube 13 Cover housing 27 Manifold plate 14 Housing screw Figure F 2 1d Pipette Components ...

Page 43: ...ring Replacement The O rings may require replacement if liquids have been over aspirated In this case the O rings should be removed prior to autoclaving Spare O ring packs are available through Matrix for all pipette volumes 1 Remove old O rings 1250 µl 850 µl 250 µl Pipettes O rings can be removed from the pistons by squeezing one side of the O ring and lifting it out of the O ring groove on the ...

Page 44: ...plied in the Preventive Maintenance Autoclave Kit to apply a thin layer of lubrication approximately 0 5 inches approx 1 3 cm down into each piston hole that requires it Spread the lubricant evenly along the inner wall of each cylinder by using a circular motion with the wooden applicator see Figure F 2 3b 125 µl 30 µl 12 5 µl Pipettes Spread the lubricant directly onto the pistons approximately 0...

Page 45: ...re that the lot number of the cylinder is facing up and the painted section of the piston backplate is facing down Also care should be taken to keep the O rings from being pinched when inserting the pistons into the cylinder 2 Cross Tube Replacement Refer to the following figure and table for proper connection of cross tubes from the tip fittings to the cylinder positions Figure F 2 3d Reassemble ...

Page 46: ...nto the hole of the motor coupling This can be accomplished by holding the piston cylinder assembly at a slight angle while inserting the motor screw into the hole of the motor coupling Slowly spin the motor screw until the flat end of the motor screw is fully engaged into the motor coupling hole see Figure F 2 3e The cylinder can now be seated between the plastic ribs in the main housing NOTE The...

Page 47: ...old plate and tubing one complete turn counterclockwise see Figure F 2 3f Insert the manifold assembly into the main housing support ribs 5 Slide the ejector comb over the tip fittings with the slots on the back of the comb exposed see Figure F 2 3g Figure F 2 3e Installing Piston Cylinder Assembly Figure F 2 3f Rotating Manifold Assembly Figure F 2 3g Attaching Ejector Comb ...

Page 48: ...models install the trigger prior to connecting the ejector comb NOTE Be careful not to pinch the cross tubes when inserting the ejector trigger Test the ejector trigger to ensure that the ejector comb moves down when the trigger is pressed hold the ejector comb firmly in place while testing the trigger 7 Place the cover housing onto the main housing and fasten the three screws 8 Re attach the colo...

Page 49: ... 8 12 Multichannels 2 NiMH Item No 6092 Single Channel 1 NiMH Pack Item No 2092 16 Channel only 1 Lithium Ion Battery Item No 2094 Note When replacing batteries ensure the pipette is turned off before removing the battery case F 3 1 Installing Replacing Nickel Metal Hydride Batteries F 3 1A Installing Replacing Single Channel Electronic Pipette Batteries 1 Remove screws Use a small flathead screwd...

Page 50: ...t the wire is looped under the battery pack and the connector is above it refer to Figure F 3 1Ac NOTE The battery pack fits very snugly in the main housing therefore ensure that the pack is oriented as shown in Figure F 3 1Ac The connectors for the battery pack and the pipette should be on opposite sides 6 Reconnect wire connectors Reconnect the wire connectors above the battery pack Gently push ...

Page 51: ...to avoid pinching the cables Hold assembly together firmly while resecuring the two screws 8 Charge battery Charge the new battery before using the pipette see Section F 4 Recharging Batteries F 3 1B Installing Replacing Multichannel Electronic Pipette Batteries The Matrix Electronic pipette is shipped with Nickel Metal Hydride batteries packaged separately Install the batteries and recharge them ...

Page 52: ...tom of the battery case to remove the batteries see Figure F 3 1Bb 3 Install new batteries Insert the spacer into the bottom of the battery box as shown in Figure F 3 1Bc Insert new batteries by placing the positive end into the battery case first then the negative end in place over the spacer an exploded view of the assembly is shown in Figure F 3 1Bc or the diagram in the battery case Ensure tha...

Page 53: ...ition your thumb over the thumb grips see Figures F 3 2a b and brace your fingers against the front of the keypad Apply pressure to the panel with the thumb grips to release latch Carefully slide battery case out of the pipette allowing sufficient slack for attached wires of the battery 2 Remove Console Cover Unclip the two snaps on console cover assembly then unhook the end of console cover assem...

Page 54: ... cable Refer to Figure F 3 2f 4 Unclasp Battery Connector Lift one side of battery connector with a small flathead screwdriver Grasp all three wires and pull out connector see Figures F 3 2f g CAUTION Be careful not to tear the Flex Cable Figure F 3 2f Lift Battery Connector Figure F 3 2g Unclasp Battery Connector Figure F 3 2e Remove Console Cover ...

Page 55: ...ngle channel pipettes which do not have this ability You can operate the pipette while it is recharging or you can operate it solely with the power transformer without batteries The batteries can be recharged approximately 400 500 times To extend battery life the pipette will turn off automatically if it is not used within ten minutes 1 Attach transformer Attach the cable end of the transformer in...

Page 56: ...To extend battery life the pipette will turn off automatically if it is not used within ten minutes Rapid Charge The battery should be charged for 1 5 to 2 hours to achieve a full charge Refer to the following table for partial charge times Amount of Charge Time 80 1 25 hour 95 1 5 hours 100 2 hours max The battery pack for the 16 channel Electronic pipette features an indicator light to indicate ...

Page 57: ... the battery be recharged whenever it is not being used instead of waiting until the battery is completely discharged Completely draining the battery will not shorten the life expectancy However recharging before fully discharged will prolong the battery life CAUTION Avoid excessively charging the batteries when the pipette is not in use excluding 16 channel pipettes Excessive charge to the batter...

Page 58: ...ormal maintenance No other warranty is expressed or implied including the warranty of merchantability Should a unit need to be returned the purchaser must pay insurance and shipping charges Thermo Fisher Scientific will assume the cost of returning the repaired unit to the purchaser This products packaging is capable of withstanding normal shipping hazards Contact Thermo Scientific Technical Servi...

Page 59: ...e the ultimate in convenience with a single documentation form to select service options and certify proper decontamination Free shipping via FedEx ensures pipettes arrive at our facility quickly reducing the time away from your lab Contact your sales representative or call 800 345 0206 to have these pre paid pipette shipping boxes delivered right to your door Accuracy Precision Every pipette is v...

Page 60: ...lidation sheet or CD containing results of performance validation Validation If a pipette fails validation on site we will offer to repair it at our facility and if necessary deal with the original manufacturer to ensure any major repairs are completed properly Service and Support Our support specialists work in partnership with you to keep your pipettes performing at their peak We understand how ...

Page 61: ... C 10 Hr 70 30 IPA Water 10 Bleach Ethylene Oxide Autoclave 121 C 15 min Multichannel Pipettes Pipette Exterior YES Wipe Wipe YES NO Piston Cylinder Assembly YES YES NO YES YES Tip Manifold YES YES NO YES YES Battery NO Wipe NO NO NO Power Supply NO Wipe NO NO NO Pipette Exterior NO Wipe Wipe NO NO Single Channel Electronic Pipettes Pipette w Battery Removed Yes Wipe Wipe YES NO Lower cylinder YES...

Page 62: ...ioned Transformer Item 6099 6096 1096 1099 9068 6098 1098 1097 9068 6098 1098 1097 9068 Standard EN61010 1 1993 Safety regulations EN55022 EN55011 1 Emissions EN50082 1 1992 Immunity EN60825 DENAN Directive 73 23 EEC Low Voltage 89 336 EEC Electromagnetic compatibility Law No 234 Electical Appliance and Material Safety Law Japanese Ministry of Economy Trade and Industry Approval mark Europe ...

Page 63: ...l 7250 7151 7152 7275 7156 250 μl TallTip 125 μl 250 μl 7280 7281 7282 7285 300 μl Tip 125 μl 250 μl 7320 7321 7322 7325 300 μl TallTip 125 μl 250 μl 7080 7081 7082 7085 1250 μl Tip 1250 μl 8050 8041 8042 8046 8045 1250 μl TallTip 1250 μl 8250 8241 8242 8246 8245 Pipet Tips Pipettes Non Sterile Sterile Sterile Filtered 12 5 μl 384 Tip 12 5 μl 384 16 Channel 7421 7422 7245 30 μl 384 Tip 30 μl 384 1...

Page 64: ...nter Key F 8 Factory Calibration Step 24 Fill 14 Fill Key 7 Hazards and Precautions 12 K Keypad 5 6 L Lubrication 34 40 M Maintenance Multichannel 35 43 Maintenance Single channel 32 35 Maintenance Procedures 31 52 Mix 15 Mix Key 7 O On Off Key 6 Open Programming Mode 13 16 Operation Keys 6 7 P PACE 9 Paced Dispense 9 22 Pipetting Trigger 5 Prog Key 6 Program Examples 18 20 Program Recall 17 Progr...

Page 65: ......

Page 66: ...iquid Handling Products 22 Friars Drive Hudson New Hampshire 03051 United States Technical Support North America Tel 800 345 0206 Email matrix technicalservice thermofisher com Hours of Operation 8 00 AM to 5 00 PM ET Europe Tel 44 0 161 486 2112 Email matrixtechcorp eu service thermofisher com Hours of Operation 08 30 to 17 00 GMT N09238 ...

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