26
Calibration
2.
Calculate display volume of the pipette:
V
p = Programmed Volume
Va
= Corrected Actual Volume (corrected for temperature)
C
p = Calibration Point (
each pipette handle has a unique calibration
point, see table D.2.2: Calibration Specifications.
)
D
v = Display Volume
3.
Enter the CAL mode on the pipette by pressing the Special Function key
(
use a pipette tip to press the key
) until “EASY” appears on the display.
4.
Press the [Vol] key to display “CALC” (
pressing the [Vol] key again will
return you to the “EASY” mode
).
5.
Press the [Enter] key to display the calibration point.
6.
Press the [Vol] key to enter the Display Volume (Dv) calculated above in
step 2.
7.
Press the [Enter] key to set the calibration point. The display shows “CAL”
then “SET” to confirm the new calibration point, and returns to the current
pipetting program.
Example: Calibrate a 1250 µl 8-channel pipette.
Water temperature = 24 °C
Programmed Volume (Vp) = 1000 µl
Average of Actual Volume = 990 µl
Corrected Actual Volume (Va) = 990 x 1.004 = 994 µl
Calibration Point (Cp) = 1000
D
v = x
Vp
Va
C
p
D
V
= x Cp
V
p
Va
D
V
= x
1000
1000
µl
994
µl
D
V
= 1006
