Thermo Scientific Invitrogen Qubit Flex Fluorometer User Manual Download Page 37

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Qubit

™

Flex Fluorometer User Guide

(Optional)

Re-run

standards after

calibration error

In the

Calibration error

screen, press

Done

If you wish to re-run the standards, or run new standards, prepare a fresh set
of standards, then load Standard #1 into the instrument.

Press

Run standards

, then repeat the calibration procedure (page 25).

Summary of Contents for Invitrogen Qubit Flex Fluorometer

Page 1: ...For Research Use Only Not for use in diagnostic procedures Qubit Flex Fluorometer USER GUIDE Catalog Number A44984 A45924 A45925 Publication Number MAN0018186 Revision A 0 ...

Page 2: ...OM THIS DOCUMENT INCLUDING YOUR USE OF IT Revision history MAN0018186 Revision Date Description A 0 11 October 2019 New user guide Important Licensing Information This product may be covered by one or more Limited Use Label Licenses By use of this product you accept the terms and conditions of all applicable Limited Use Label Licenses Trademarks All trademarks are the property of Thermo Fisher Sci...

Page 3: ...ulator to determine the assay range 26 Use the Reagent Calculator to prepare Qubit Working Solution 30 Run standards for assay calibration 32 Read samples 36 Results 43 Optional Use the Molarity Calculator to determine sample molarity 45 Optional Use the Normalization Calculator to determine how to dilute the samples to the same molarity concentration or mass 48 4 Manage data 58 View detailed samp...

Page 4: ...nsiderations 99 Appendix B Ordering information 101 Qubit Flex Fluorometer and accessories 101 Appendix C Safety 102 Symbols on instruments 102 Safety labels on instruments 103 General instrument safety 104 Chemical safety 105 Chemical waste safety 106 Electrical safety 107 Biological hazard safety 108 Safety Electromagnetic compatibility EMC and Environmental standards 109 Documentation and suppo...

Page 5: ... Guide are listed below For safety alert words and symbols used in Thermo Fisher Scientific user documentation see page 4 Convention Use Bold Bold text indicates user action For example Click Run Right arrow symbol indicates a menu choice and separates successive commands you execute or select from a drop down or shortcut menu For example Select Settings Instrument Settings User attention words Tw...

Page 6: ... situation that if not avoided could result in death or serious injury DANGER Indicates an imminently hazardous situation that if not avoided will result in death or serious injury This signal word is to be limited to the most extreme situations Except for IMPORTANT safety alerts each safety alert word in a Thermo Fisher Scientific document appears with an open triangle figure that contains a haza...

Page 7: ...nstrument The complete user guide is available for download at thermofisher com qubit See page 6 for the description and specifications of the Qubit Flex Fluorometer Upon receiving the instrument Examine the instrument carefully for damage incurred during transit Ensure that all parts of the instrument including the accessories listed above are included with the product Damage claims must be filed...

Page 8: ...ates samples that are in the extended range or out of range Saves sample data as a CSV comma separated value file On board Reagent and Range Calculators provide instructions to prepare Qubit working solution using your sample and standard inputs and to select the most accurate assay for your expected concentration range On board Molarity and Normalization Calculators allow you to calculate molarit...

Page 9: ...containing your samples into the fluorometer for analysis USB drive ports Type A allow you to transfer and save data to your computer using a USB flash drive or wirelessly to a network drive or a Connect account using the Wi Fi dongle supplied with the instrument Power inlet connects the Qubit Flex Fluorometer to an electrical outlet using the supplied power cord and the appropriate plug LAN port ...

Page 10: ...g temperature 10 30 C Operating humidity 15 80 non condensing Pollution degree The instrument has a Pollution Degree rating of II The instrument may only be installed in an environment that has nonconductive pollutants Typical environment with a Pollution Degree II rating are laboratories and sales and commercial areas Technical specifications Dynamic range 4 orders of magnitude Processing time 3 ...

Page 11: ...reen with high resolution color display Output ports 3 USB ports Networking capability Connection via the LAN RJ 45 port using an Ethernet cable or wirelessly using the supplied Wi Fi adaptor Power supply AC adaptor with country specific power cords USB drive Capacity 4 Gigabyte ...

Page 12: ...at is appropriate for the electrical outlet configuration in your country Powering the instrument with an unapproved power cord can irreversibly damage the instrument Note that the power cords for the Qubit Flex Fluorometer are not interchangeable with those for the other Qubit Fluorometer models 5 The instrument automatically powers on first displaying the splash screen then the End User License ...

Page 13: ...e Settings Instrument Settings Language screen page 85 1 On the Language displayed screen select the Language you want your instrument to display then press Next Available options are English French German Italian Chinese Japanese and Spanish 2 On the Date Time screen select the Time Zone set the Date and Time in the desired format then press Next Note For detailed instructions on how to configure...

Page 14: ...he Settings Instrument settings Network connection screen page 78 1 On the Network Connection screen select Wireless or Wired connection If you wish to use the instrument without joining a network press Skip You can always join a network and configure network settings later 2 Depending on your choice the instrument displays the Choose Network or the IP Configuration screen for Wireless and Wired c...

Page 15: ...r detailed instructions on how to join a network wireless or wired and configure network options see Network connection page 78 4 On the Network Connection screen click Network Drive to map the location on the network where you want to save your Qubit Flex files For detailed instructions on how to map a network drive see Map a network drive page 81 Note You must have an established network connect...

Page 16: ...nnect account Select the assays to perform 1X dsDNA High Sensitivity HS dsDNA High Sensitivity HS dsDNA Broad Range BR RNA High Sensitivity HS RNA Broad Range BR Protein Oligo ssDNA microRNA Access saved data Filter delete or export data Configure instrument settings Use the Reagent Calculator to determine the exact volumes of Qubit buffer and reagent required to prepare the Qubit working solution...

Page 17: ...ation about your Qubit Flex Fluorometer including the currently installed software version To access the About Instrument screen 1 On the Home screen press Settings 2 On the Settings screen press About Instrument to display the About Instrument screen 3 Press Close or Back to return to the Settings screen ...

Page 18: ...ped network location and it is also required to connect to your Connect account If you wish you can skip this step and create a profile later from the Profile screen 1 On the Home screen press the Profile button on the top left corner of the screen to open the Sign In screen 2 If you are new to the instrument and have not yet created a profile press Get Started to open the Get Started screen then ...

Page 19: ...ss Skip You can always create an instrument profile later 4 Press the User name field enter the desired user name for the profile 1 20 alphanumeric characters no spaces then press Done 5 Press the PIN field enter a 4 digit PIN then press Done 6 Enter the PIN in the Confirm PIN field then press Done 7 Press Create to create the local instrument profile ...

Page 20: ...t have a Connect account or create one To create your Connect account online or to sign in to your existing account go to thermofisher com cloud 1 Ensure that you are connected to the network on your Qubit Flex instrument page 12 2 On the Home screen press the Profile button on the top left corner of the screen to open the Sign In dialog 3 On the Sign In screen press Get Started to open the Get St...

Page 21: ...d on PC Step 6 page 20 Instrument Manually Step 7 page 21 5 To connect to your Thermo Fisher Connect account with the Instrument Connect App on your mobile phone a Download the Instrument Connect Mobile App from the application store on your mobile phone b Press Instrument Connect App on the Connect to the Cloud screen then follow the steps on the Qubit Flex instrument When finished go to Step 8 p...

Page 22: ...mofisher com cloud and sign in to your Thermo Fisher Connect account b On the Connect dashboard press the Instrument Connect button Instrument Connect screen opens c On the Instrument Connect screen press Add an instrument Add an instrument dialog opens d From the instrument type dropdown select Qubit_Flex then press Next ...

Page 23: ... Qubit Flex instrument into the Add an instrument dialog then press Send g When finished go to Step 8 page 22 7 To connect to your Thermo Fisher Connect account with manually with the Qubit Flex instrument a Press Instrument Manually on the Connect to the Cloud screen on the Qubit Flex instrument see page 19 b Enter your User name and Password for your Thermo Fisher Connect account then press Conn...

Page 24: ...t Flex Fluorometer User Guide 8 When you have signed in to your Thermo Fisher Connect Account the Profile button on the Home screen becomes blue When signed in you can export your data to your Connect account ...

Page 25: ...ate air bubbles when mixing the sample or standard with the working solution Incubate the tubes for the Qubit DNA and RNA assays for 2 minutes after mixing the sample or standard with the working solution Incubate the tubes for the Qubit protein assays for 15 minutes after mixing the sample or standard with the working solution If you are performing multiple readings of a single tube remove the tu...

Page 26: ... the assay you are using or the Qubit Flex Fluorometer Quick Reference Card QRC Pub No MAN0018187 Optional USB drive USB cable or Ethernet cable for data transfer supplied with the instrument or available separately Note You can also transfer your data to a network location or your Connect account wirelessly if you have set up a wireless connection Sign in to your profile 1 Press the Profile butto...

Page 27: ...name indicates that the profile has an associated Connect account When the Connect icon is blue the profile is signed in to the associated Connect account 4 Press PIN enter the PIN for your profile then press Enter 5 Press Sign in to sign in to your account and return to the Home screen The blue profile button indicates that you have signed in to your account ...

Page 28: ... and high ranges based on your sample volume Knowing the assay range can help you determine which Qubit assay provides the most accurate quantification based on your sample volume and estimated sample concentration Use the Assay Range Calculator 1 On the Home screen press Calculators 2 On the Select Calculator screen press Assay Range to open the Assay Range Calculator 3 Press Output sample unit t...

Page 29: ...y range 5 Enter the sample volume to be used directly in the sample volume text box You can also use the and buttons or adjust the sample volume wheel The Assay Range Calculator displays the Core sample concentration range for the selected assay and the Extended low and high ranges based on your input ...

Page 30: ... observe how changes in the sample volume affect Core and Extended accuracy ranges for the assay dsDNA HS Assay range for 20 µL sample volume dsDNA HS Assay range for 5 µL sample volume Note The sample volume used 1 20 µL changes the assay accuracy range For highest accuracy use the maximum sample volume that would keep the concentration measurements within the core range Note that a different sam...

Page 31: ... the core and extended ranges and guidelines for obtaining best assay results 8 Press Close to return to the Assay Range Calculator 9 Optional If desired repeat the procedure for another assay to determine whether it would provide more accurate results in the expected concentration range 10 Press Done to return to the Home screen ...

Page 32: ...equired to prepare the Qubit Working Solution for your samples and standards Use the Reagent Calculator 1 On the Select Calculator screen press Reagent to open the Reagent Calculator 2 Enter the total number of samples and standards that you plan to run 3 Optional Select Include overage if you want to include reagents for three additional tubes 600 µL in the total calculated volume ...

Page 33: ...he Qubit Working Solution with these inputs Note You can change the total number of tubes that you plan to run or the overage selection on this screen 5 Press Done to return to the Select Calculator screen 6 Press the Back button to return to the Home screen or press Assay Range to open the Assay Range Calculator page 26 ...

Page 34: ...IMPORTANT Be sure to use the appropriate standards for your assay For best results run new standards each time you perform an assay Run new standards 1 On the Home screen press to select the Assay to perform To view the next screen of available assays swipe to the left To return to the previous page swipe to the right 2 When prompted press Read standards run samples to read new standards Note To a...

Page 35: ...rompted insert Standard 2 then press Run standards 5 For Qubit protein assays only When prompted insert Standard 3 then press Run standards The calibration is complete after Standard 2 is read or after Standard 3 for the Qubit protein assay and the software displays the results see Calibration results page 34 6 If your calibration is successful press Next to proceed to Read samples page 36 ...

Page 36: ...raph is displayed In the Fluorescence vs Concentration graph the standard data points are connected by a line and open circles represent correct standards If the calibration is not successful Calibration error message is displayed If you receive the Calibration error message you can re run the standards see Re run standards after calibration error page 35 ...

Page 37: ...ter calibration error 1 In the Calibration error screen press Done 2 If you wish to re run the standards or run new standards prepare a fresh set of standards then load Standard 1 into the instrument 3 Press Run standards then repeat the calibration procedure page 25 ...

Page 38: ...appropriate amount of time after mixing them with the working solution 2 minutes for the Qubit DNA and RNA assays 15 minutes for the Qubit protein assay Insert samples 1 When prompted load the tube strip containing the samples as shown in the Insert samples screen If you have fewer than 8 samples press to deselect the tube positions that do not contain a sample All 8 tubes contain samples No sampl...

Page 39: ...range information on the screen automatically changes to reflect the new core and extended accuracy ranges based on the sample volume dsDNA HS Assay range for 10 µL sample volume dsDNA HS Assay range for 20 µL sample volume Note The sample volume used 1 20 µL changes the assay accuracy range For highest accuracy use the maximum sample volume that would keep the concentration measurements within th...

Page 40: ...ags to your sample run Step 3 page 39 Add Sample IDs Step 6 page 40 The information you have entered will be available on the Data Details of your samples page 62 Note You can open the More Options screen from the Insert Samples page 36 or the Sample Volume page 37 screens 2 To enter an assay kit lot number press the Enter assay kit lot text box enter the assay kit lot number then press Enter ...

Page 41: ... box to open the Create New Tag screen enter the new tag then press Enter The new tag will be added to the list of available tags on the Add Tag screen Note To filter the list of available tags for the last used tag press Last Used To display all existing tags alphabetically press Alphabetical To sort the list of available tags alphabetically in ascending or descending order press the Tag column h...

Page 42: ...tton changes to Edit Tags Note To return to the More Options screen without applying a tag to your samples press Cancel To delete an existing tag select the tag from the list of available tags then press Delete To change the tag applied to your sample run press Edit Tags on the More Options screen 6 To add sample IDs to your samples press Add Samples IDs then select Cloud your Connect account see ...

Page 43: ...the file containing the sample IDs from the list of available files then press Apply 8 Press OK at the confirmation page 9 When finished entering assay kit lot number and applying tags and sample IDs press Done at the More Options screen The assay screen displays the new information added to your samples at the bottom of the screen To go back to the assay screen without applying the new informatio...

Page 44: ...ton to unselect it The Results screen lists the concentration of each original sample using the output units selected at the beginning of the assay Note By default the Results screen displays the measurements in graph view However the graph settings are sticky so that if you close the graph the next time anyone runs an assay the graph view is hidden and the results are shown in list form 3 To run ...

Page 45: ...ration of each original sample below the graph In the graph Open circles represent correct standards Blue circles represent samples that fall within the assay s core range Orange circles represent samples that fall within the assay s extended range Red circles represent samples that fall outside the assay s range 2 To view a sample on the Fluorescence vs Concentration graph press the desired sampl...

Page 46: ...ginning of the assay If the concentration of a sample is within the assay s extended range the concentration value is displayed in orange and an extended range message and an orange circle are displayed next to the concentration value If the concentration of a sample is outside of the assay s range an out of range message and a red circle are displayed next to the sample 4 To display the results i...

Page 47: ...calculate the molarity of your samples based on nucleic acid length and their measured concentration Use the Molarity Calculator 1 On the Results screen press Calculators then select Molarity to open the Molarity Calculator 2 On the Molarity Calculator screen press the Desired units fields to select the input and output units Input units Output units ...

Page 48: ... uses a default value of 660 g mol for the average molecular weight of one DNA base pair To change the auto populated MW value press the Molecular weight field and enter the desired average molecular weight of your sample 3 Press Length bp field for Sample 1 S1 enter the length bp of Sample 1 then press Enter 4 If all your samples have the same length select Auto populate DNA length ...

Page 49: ...nits that you have selected Note When you press Calculate the instrument saves the data from molarity calculations with the sample data in the CSV file 6 To export your results press Export The instrument exports the complete CSV file with all sample data including the molarity calculation results To go back to the Calculator screen press the Back button ...

Page 50: ...ormalization Calculator 1 On the Results screen press Calculators then press Normalization 2 On the Normalization Calculator screen select Molarity to determine how to dilute your samples to the same final mass and volume page 49 Concentration to determine how to dilute your samples to the same final concentration page 52 Mass to determine how to dilute your samples to the same final mass and volu...

Page 51: ...he same molarity 1 On the Normalization Calculator screen select Molarity 2 Enter the Final sample mass and select units 3 Enter the Final sample volume and select units then press Enter Note The minimum allowed sample volume on the Normalization Calculator is 5 µL ...

Page 52: ...e data from normalization calculations with the sample data in the CSV file 5 Press the right arrow to view page 2 of results which displays the required sample buffer dilution before mixing Required Dilution if applicable and the sample concentration after the dilution Diluted conc If dilution is not required before mixing then N A is displayed in the Required Dilution and Diluted conc columns fo...

Page 53: ...he normalization calculation results 9 Otherwise press Done to close the Normalization calculator and go back to the Calculator screen Note If your sample needs further dilution before mixing to achieve the desired final molarity the required sample buffer dilution is indicated in the Add sample column in red and in the Required Dilution column on page 1 and 2 of calculation results respectively I...

Page 54: ...oncentration 1 On the Normalization Calculator screen select Concentration 2 Enter the Final sample concentration and select units 3 Enter the Final sample volume and select units then press Enter Note The minimum allowed sample volume on the Normalization Calculator is 5 µL ...

Page 55: ...e data from normalization calculations with the sample data in the CSV file 5 Press the right arrow to view page 2 of results which displays the required sample buffer dilution before mixing Required Dilution if applicable and the sample concentration after the dilution Diluted conc If dilution is not required before mixing then N A is displayed in the Required Dilution and Diluted conc columns fo...

Page 56: ...g the normalization calculation results 9 Otherwise press Done to close the Normalization calculator and go back to the Calculator screen Note If your sample needs further dilution before mixing to achieve the desired final concentration the required sample buffer dilution is indicated in the Add sample column in red and in the Required Dilution column on page 1 and 2 of calculation results respec...

Page 57: ...e mass and volume 1 On the Normalization Calculator screen select Mass 2 Enter the Final sample mass and the desired units 3 Enter the Final sample volume and the desired units then press Enter Note The minimum allowed sample volume on the Normalization Calculator is 5 µL ...

Page 58: ...e data from normalization calculations with the sample data in the CSV file 5 Press the right arrow to view page 2 of results which displays the required sample buffer dilution before mixing Required Dilution if applicable and the sample concentration after the dilution Diluted conc If dilution is not required before mixing then N A is displayed in the Required Dilution and Diluted conc columns fo...

Page 59: ...cluding the normalization calculation results Otherwise press Done to close the Normalization calculator and go back to the Calculator screen Note If your sample needs further dilution before mixing to achieve the desired final mass and volume the required sample buffer dilution is indicated in the Add sample column in red and in the Required Dilution column on page 1 and 2 of calculation results ...

Page 60: ...een press Data The Data screen opens and displays the list of data sets that are saved in the instrument 2 By default the data sets are arranged by date in descending order To sort the data sets press the appropriate category in the header row To sort the data sets by date in ascending order press Date To sort the data sets by date in descending order press Date again To sort the data sets by Assa...

Page 61: ...ter User Guide 59 Optional Filter data sets 1 To filter data sets by Assay or Tag press Actions to open the Actions screen then select Filter data 2 On the Filter Data screen press Assay then select the Assay of interest ...

Page 62: ...d applied a tag to the assay page 38 select the Tag from the list Otherwise go to step 4 4 Press Apply to filter the data list by the assay and tag you have selected Only the data sets that satisfy the filter criteria are displayed in the Data screen ...

Page 63: ...he Data screen filtered or not filtered press the data set of interest The Data set screen opens and displays a list of samples in that run 2 To view the sample details press the sample of interest A Data details screen opens To view sample details that do not fit in the screen scroll down ...

Page 64: ...e Date of last read standards Assay date Sample volume Original sample concentration Dilution factor Qubit tube sample concentration Excitation channel Assay name Optional information Tags Reagent lot etc Sample RFU value RFU Relative Fluorescence Units Molarity calculation information units nucleic acid length MW molarity ...

Page 65: ... Edit sample name Edit sample name 1 On the Data screen select the data set of interest then select the sample you want to rename 2 On the Data details screen press the Sample set field indicated by red arrow Edit Sample Name screen opens ...

Page 66: ... name then press Enter Data Details screen reappears and displays the new sample name 4 If you wish to rename all of the samples in the data set press the Next button to go the next sample instead of pressing Enter at step 3 then enter the new name for that sample ...

Page 67: ... all remaining samples When finished renaming all the samples press Enter Data Details screen reappears and displays the new sample name 6 Press the Back button to return to the Data screen for the assay All of the samples display the new sample names ...

Page 68: ...USB flash drive or save to your Connect account or a network drive wirelessly or via the Ethernet cable You can then view the file in any spreadsheet program Export data 1 On the Home screen press Data to open the Data screen 2 To export entire data sets press the check box to the left of each data set that you wish to export You can select multiple data sets To select all data sets to export pres...

Page 69: ...to view individual samples in the data set 4 Press the check box to the left of the samples that you wish to export You can select multiple samples to export To select all samples in the data set to export press the blue check icon on the header row Three samples in the data sets selected All samples in the data set selected ...

Page 70: ...data screen select the Export method Available options are Cloud i e your Connect account USB and Network Drive To export data to a USB drive insert the USB drive into the Qubit Flex Fluorometer To export data to your Connect account or a network drive ensure that the instrument is connected to the network wirelessly or via an Ethernet cable ...

Page 71: ...Qubit Flex Fluorometer User Guide 69 7 Press Export to export the data The numeric data is automatically saved as a CSV file You can open the CSV file using any spreadsheet program ...

Page 72: ...ll data sets press the blue check icon on the header row To delete only individual sample files from a data set press the data set of interest to view individual samples in the data set then press the check box to the left of the samples you wish to delete Select data sets to delete Select Sample files to delete 3 After you have selected the data sets or the samples press Actions then select Delet...

Page 73: ...Fluorometer User Guide 71 4 Press Delete A warning screen appears 5 Press Delete to permanently delete the sample data or data set 6 Press Cancel to return to the screen previously viewed without deleting any data ...

Page 74: ...rometer from the Settings Instrument Settings screen Sleep mode page 73 Brightness page 74 Date Time page 75 Network Connection page 78 Reset instrument page 84 Language page 85 Cloud region page 86 Access the Instrument Settings screen 1 On the Home screen press Settings 2 On the Settings screen press Instrument settings to display the Instrument settings screen ...

Page 75: ...n the Instrument Settings screen page 72 press Sleep Mode 2 To change the time of inactivity before the instrument goes into sleep mode press Edit Time field then enter the time between 1 minute and 60 minutes 3 To disable the sleep mode toggle the Enable Sleep Mode switch to the Off position 4 Press Done to save the changes and return to the Instrument Settings screen Press Cancel or Back to retu...

Page 76: ...t Settings screen page 72 press Brightness 2 Move the Brightness slider up or down to adjust the brightness of the display 3 Press Done to save the changes and return to the Instrument settings screen Press Cancel or Back to return to the Instrument settings screen without saving the changes ...

Page 77: ...it Flex Fluorometer User Guide 75 Date and Time Set the date and time 1 On the Instrument Settings screen page 72 press Date Time 2 Press Time Zone then select the time zone for your location from the list ...

Page 78: ... Guide 3 Press Date then choose MM DD YYYY DD MM YYYY or YYYY MM DD for the date format 4 To set the date press the DD MM and YYYY fields to enter the Day Month and Year 5 Press Enter when finished entering the date then press Done ...

Page 79: ...r for the time format 12 Hour format 24 Hour format 7 To set the time press the Hours and Minutes fields to enter the Hours and Minutes If you have chosen the 12 Hour format select AM or PM 12 Hour format 24 Hour format 8 Press Enter when finished entering the time then press Done ...

Page 80: ...n Ethernet cable After you have joined a network you can also connect to Thermo Fisher s Connect cloud based platform to store and access your data files 1 To access the Network Connection screen press Settings Instrument Settings then select Network connection 2 The Network Connection screen opens To connect to a Wi Fi network go to page 79 To establish a wired connection to a local area network ...

Page 81: ... disconnecting and reconnecting the power supply 2 Press Settings Instrument Settings then select Network connection 3 On the Network Connection screen press Wireless The instrument searches for available wireless networks within range 4 On the Choose Network screen press the network you want to join 5 If required enter the appropriate security credentials then press Join After the connection is e...

Page 82: ...tandard Category 6 Ethernet cable 2 On the Instrument Settings screen press Network connection then select Wired 3 On the IP Configuration screen select DHCP or Static 4 If you have selected Static enter the static IP address MAC address Subnet mask Default Gateway address and primary and secondary DNS server addresses for the LAN port 5 Press Done to join the local area network ...

Page 83: ...ected to an active network and that you have signed in to your profile page 24 2 On the Instrument Settings screen press Network connection then select Network Drive 3 On the Network Drive screen press Drive location enter the location of the drive to save your Qubit Flex files then press Enter ...

Page 84: ...82 Qubit Flex Fluorometer User Guide 4 Press Domain name enter the domain name where the drive is located then press Enter 5 Press User name enter your user name for the network drive then press Enter ...

Page 85: ...Qubit Flex Fluorometer User Guide 83 6 Press Password enter your password for the network drive then press Enter 7 When finished entering all the required fields for the Network Drive press Connect ...

Page 86: ...ment Settings screen page 72 press Reset instrument to display the Reset Qubit Flex screen 2 To return the instrument to its default factory settings press Reset After the reset is complete all data user defined instrument settings and custom assays are removed and the instrument displays the Home screen Press Cancel or Exit to return to the Instrument settings screen without saving the changes IM...

Page 87: ...Instrument Settings screen page 72 press Language to display the Language screen 2 Press to select the desired language Available options are English French German Italian Chinese Japanese and Spanish 3 When prompted press Yes to confirm the change and return to the Instrument settings screen If you do not want to change the language settings press Cancel or Exit to return to the Instrument settin...

Page 88: ... then press Change 3 When prompted press Change to close the warning screen then press Change again to change the cloud region of the instrument The instrument will restart after changing the cloud region If you do not want to change the cloud region press Cancel to return to the previous screen Note If you change the cloud region of the instrument you must relink all instrument accounts ...

Page 89: ...ontaminating the instrument IMPORTANT Using a cleaning or decontaminating method other than that specified by the manufacturer may result in damage to the instrument Clean the surface of the Qubit Flex Fluorometer with a damp cloth To clean the touchscreen disconnect the power cable and clean the touchscreen with a soft cloth lightly moistened with LCD liquid crystal display cleansing detergent Cl...

Page 90: ...sing a network drive ensure that the instrument is connected to the network wirelessly or via an Ethernet cable Update the software 1 On the Home screen press Settings then select Software update 2 On the Software Update screen select Cloud USB or Network Drive If you have selected Cloud or Network drive enter your credentials to sign in The instrument searches your Connect account the USB drive o...

Page 91: ...date insert the correct USB drive into the instrument then press Retry 3 If a new update is available and the appropriate files are detected the instrument displays Software update is available Press Update to view the available versions of the software 4 Select the software version you want install on the instrument for the update then press Update 5 When prompted press Restart to complete the so...

Page 92: ... the use of the Qubit Flex System Verification Assay Kit Cat No Q33254 Perform the system verification when a problem with the instrument is suspected It is not necessary to perform the verification regularly Perform System verification test 1 On the Home screen press Settings then select System Verification 2 On the System Verification screen press Next ...

Page 93: ...ube strip 1 200 µL of Green Fluorescence Reagent to each tube of tube strip 2 and 200 µL of Far Red Fluorescence Reagent to each tube of tube strip 3 then press Next 5 When prompted ensure that the sample chamber is empty and the lid is closed then press Start test to run the Qubit Flex hardware test Step 1 of System Verification This test takes approximately 15 seconds ...

Page 94: ...e found System Verification Pass message appears Press Close to return to the Settings screen or press Next to view the System Verification Report page 93 If errors are found Error Reading Reagents message appears Verify that the test was run with the lid closed then press OK to re run the test with the tube strips in the correct order If the System Verification Failed message persists after re ru...

Page 95: ...r press Data Log to view and export the available data logs page 93 The System Verification Report shows the pass fail status of the instrument components Far Red Fluorescence Channel Fail All Pass 11 Press Close to return to the Settings screen or press Data Log to view the available data logs ...

Page 96: ...an select multiple Data Logs for export 13 Select Cloud Thermo Fisher Connect cloud based platform USB or Network Drive for the location where you want to save the PDF report of the Verification Assay Test Results 14 To delete a Data Log select the desired Data Log press Actions then press Delete You can select multiple Data Logs for deletion ...

Page 97: ...not keep the time setting which indicates the need to replace the battery Replace battery 1 Disconnect the Qubit Flex Fluorometer from the power source 2 Remove the four screws as indicated by the red arrows on the bottom chassis of the Qubit Flex instrument using a Phillips head screwdriver 3 Flip the instrument so that the top chassis is facing up 4 Open the instrument slightly 3 cm from the bot...

Page 98: ...emove it 6 Insert the new 3 V CR2450 battery to the battery housing 7 Arrange two cable assemblies into the groove on the bottom chassis place the top chassis on the bottom chassis so that the slots for the screws align properly then tighten the four screws on the bottom chassis using a Phillips head screwdriver ...

Page 99: ... page 100 Be careful not to spill sample into the sample chamber Promptly wipe any spills The Qubit assays are very sensitive and even small amounts of material from a previous sample may result in errors Use a clean Qubit Flex Tube Strip for each reading The tube must be clean and dry on the outside when taking readings Moisture and condensation on the tube surface can lead to reading errors Minu...

Page 100: ...ure that you have prepared the Qubit working solution correctly 1 200 dilution using the buffer provided in the kit Ensure that you have prepared the standard tubes correctly 10 µL of each standard in 190 µL of Qubit working solution Ensure that the standard and sample tubes are filled to 200 µL Protect the Qubit reagent and working solutions from light Select the correct Qubit Flex Fluorometer as...

Page 101: ...solution After this incubation period the fluorescence signal is stable for 3 hours at room temperature for all nucleic acid assays except the Qubit ssDNA assay which is stable for up to 30 minutes The Qubit protein assay requires 15 minutes of incubation for a stable signal For greatest accuracy in the protein assay the incubation time of the samples should be within 10 minutes of the incubation ...

Page 102: ...bit dsDNA BR assay Qubit dsDNA HS Qubit ssDNA Qubit RNA HS and Qubit protein assays show similar sensitivities over the same range Qubit Flex Fluorometer calibration For each assay you have the choice to run standards for a new calibration or to use the values from the previous calibration As you first use the instrument perform a new calibration each time As you become familiar with the assays th...

Page 103: ...national Power Supply replacement 1 each A36204 Qubit Flex Tube Strips 125 strips Q33252 Qubit Flex Reservoir 10 mL 100 each Q33253 Qubit Flex System Verification Assay Kit 1 kit Q33254 Qubit RNA BR Assay Kit 20 1 000 ng 100 assays 500 assays Q10210 Q10211 Qubit RNA HS Assay Kit 5 100 ng 100 assays 500 assays Q32852 Q32855 Qubit ssDNA Assay Kit 1 200 ng 100 assays Q10212 Qubit dsDNA BR Assay Kit 2...

Page 104: ...documents Symbol Description Indicates that you should consult the manual for further information and to proceed with appropriate caution Indicates the presence of an electrical shock hazard and to proceed with appropriate caution Environmental symbols The following symbol applies to all Thermo Fisher Scientific electrical and electronic products placed on the European market after August 13 2005 ...

Page 105: ...ns for handling and disposal ATTENTION Déchets dangereux Lire les fiches techniques de sûreté de matériels et la régulation locale associées à la manipulation et l élimination des déchets DANGER High voltage DANGER Haute tension WARNING To reduce the chance of electrical shock do not remove covers that require tool access No user serviceable parts are inside Refer servicing to Thermo Fisher Scient...

Page 106: ...ocation Do not install the instrument on a slant or a place prone to vibrations which induces the risk of instrument malfunction or damage of the instrument Plug the power cord firmly into the wall outlet and the instrument To avoid potential shock hazard make sure that the power cord is properly grounded Be sure to position the equipment such that it is easy to disconnect the instrument If the in...

Page 107: ...mize the hazards of chemicals Read and understand the Safety Data Sheets SDSs provided by the chemical manufacturer before you store handle or work with any chemicals or hazardous materials See Safety Data Sheets SDS page 111 Minimize contact with chemicals Wear appropriate personal protective equipment when handling chemicals for example safety glasses gloves or protective clothing For additional...

Page 108: ...ves or protective clothing For additional safety guidelines consult the SDS Minimize the inhalation of chemicals Do not leave chemical containers open Use only with adequate ventilation for example fume hood For additional safety guidelines consult the SDS Handle chemical wastes in a fume hood After emptying the waste container seal it with the cap provided Dispose of the contents of the waste tra...

Page 109: ... Power DANGER ELECTRICAL HAZARD Grounding circuit continuity is vital for the safe operation of equipment Never operate equipment with the grounding conductor disconnected DANGER ELECTRICAL HAZARD Use properly configured and approved line cords for the voltage supply in your facility DANGER ELECTRICAL HAZARD Plug the system into a properly grounded receptacle with adequate current capacity Overvol...

Page 110: ...ter des lunettes de protection approprié des vêtements et des gants In the U S U S Department of Health and Human Services guidelines published in Biosafety in Microbiological and Biomedical Laboratories stock no 017 040 00547 4 www cdc gov OD ohs biosfty bmbl4 bmbl4toc htm Occupational Safety and Health Standards Bloodborne Pathogens 29 CFR 1910 1030 www access gpo gov nara cfr waisidx_01 29cfr19...

Page 111: ...ested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference in a residential installation This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio...

Page 112: ...ity EMC Part 3 2 Limits Limits for harmonic current emissions equipment input current 16 A per phase EN 61000 3 3 2013 Electromagnetic compatibility EMC Part 3 3 Limits Limitation of voltage changes voltage fluctuations and flicker in public low voltage supply systems for equipment with rated current 16 A per phase and not subject to conditional connection IEC 61000 3 3 2008 Restriction of Hazardo...

Page 113: ...heets SDS Safety Data Sheets SDSs are available at thermofisher com support IMPORTANT For SDSs for reagents and chemicals from other manufacturers contact the manufacturer Limited product warranty Life Technologies Corporation and or its affiliate s warrant their products as set forth in the Life Technologies General Terms and Conditions of Sale found on Life Technologies website at www thermofish...

Page 114: ...thermofisher com support thermofisher com askaquestion thermofisher com 11 October 2019 ...

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