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Thermo Scientific

AI 3000/AS 3000 Sampling System - Operating Manual

AI 3000/AS 3000 II Control

This chapter provides the information to control the AI 3000/AS 3000 II from the computer
or the GC. The chapter contains also the working procedures with different injectors.

Contents

•

Control from PC

•

Control Through the TRACE GC Ultra

•

Control Through the FOCUS GC

•

Guidelines for Programming with Different Injectors

Summary of Contents for AI 3000

Page 1: ...P N 31709392 Tenth Edition March 2012 Thermo Scientific AI 3000 AS 3000 AI 3000 AS 3000 II 3000 Series Automatic Sampling System Operating Manual Home ...

Page 2: ...on received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This document is not part of any sales contract between Therm...

Page 3: ..._________________________ Address ___________________________________________________ __________________________________________________________________ City State _________________________________________Postal Code__________________________________________________________________ Country ___________________________________________________ _________________________________________________________...

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Page 5: ...nd evaluation of its products to ensure full compliance with applicable domestic and international regulations When the system is delivered to you it meets all pertinent electromagnetic compatibility EMC and safety standards Safety This device complies with EN 61010 1 2001 EN 61010 2 2002 according to Low Voltage Directive 2006 95 EC Electromagnetic Compatibility This device complies with EN 61326...

Page 6: ...Notice on the Proper Use of Thermo Scientific Instruments In compliance with international regulations Use of this instrument in a manner not specified by Thermo Fisher Scientific could impair any protection provided by the instrument Notice on the Susceptibility to Electromagnetic Transmissions Your instrument is designed to work in a controlled electromagnetic environment Do not use radio freque...

Page 7: ... Entsorgungsfirmen in allen EU Mitgliedsstaaten getroffen damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann Mehr Information über die Einhaltung dieser Anweisungen durch Thermo Fisher Scientific über die Verwerter und weitere Hinweise die nützlich sind um die Produkte zu identifizieren die unter diese RoHS Anweisung fallen finden sie unter www thermo com WEEERoHS Co...

Page 8: ...o Fisher Scientific ha contratado una o más empresas de reciclo para tratar residuos en cada Estado Miembro de la Unión Europea y estas empresas deberían reciclar o eliminar este producto Referirse a www thermo com WEEERoHS para una mayor información sobre la conformidad de Thermo Fisher Scientific con estas Directivas y para las empresas de reciclaje en su país IMPORTANTE Gli utenti italiani devo...

Page 9: ...e 3000 Series Automatic Sampling System 5 AI 3000 Auto injector Configuration 5 AS 3000 Auto sampler Configuration 6 Gemini Configuration 6 Installation 7 Technical Specifications 8 Chapter 2 Sampling Unit 9 Base 10 Turret 11 Injection Assembly 12 Vial Capture Device 13 Syringe 13 Turret Movements 14 Washing and Waste Tray 15 Rear of the Sampling Unit 15 Sample Trays 16 8 Position Sample Tray 16 1...

Page 10: ...nfiguration 37 Assembling of the Sampling System Support When a TSQ Quantum is Coupled with the GC 40 Installing the Sampling System Support on the TRACE GC Ultra 43 Installing the Spacer Inside the GC 43 Assembling of the Sampling System Support on the GC Right Side 44 Assembling of the Sampling System Support on the GC Left Side 44 Installing the Sampling System Support on the FOCUS GC 46 Assemb...

Page 11: ...th Different Injectors 70 SSL Inlet TRACE GC Ultra FOCUS GC 70 Nano Volumes Injections in S SL Inlets 71 Packing a SSL Liner 72 S SL Inlet with a Merlin Microseal 73 PTV Inlet 73 PTV Inlet with a Merlin Microseal 74 PKD and PPKD Inlets 74 Chapter 5 Maintenance 75 Current Maintenance 76 Emptying of the Waste Container 76 Cleaning of the Sample Tray Accessory 76 Cleaning the Instrument Externally 76...

Page 12: ...Contents xii AI 3000 AS 3000 Sampling System Operating Manual Thermo Scientific ...

Page 13: ...the sampling unit of the AI 3000 AS 3000 II System Chapter 3 Installation contains the instructions for the installation of the AI 3000 AS 3000 II on the TRACE 1300 TRACE 1310 TRACE GC Ultra and FOCUS GC gas chromatographs the syringe and the electrical connections with the different units of the gas chromatographic system Chapter 4 AI 3000 AS 3000 II Control provides the information to control th...

Page 14: ...e contract coverage WARNING When for technical reasons it is necessary to work on instrument parts which may involve an hazard moving parts components under voltage etc the authorized Technical Service must be contacted This type of situations can be identified because access to these parts is possible only by using a tool The removable protective covers bear a warning symbol suggesting to refer t...

Page 15: ...environment Each WARNING safety alert is preceded with this safety symbol and another appropriate safety symbol see Safety Alerts and Important Information on page xv Then it is followed with an appropriate safety precautionary message When you see a safety alert on your instrument or in the publications please carefully follow the safety instructions before proceeding CAUTION This is the safety a...

Page 16: ...urn injuries CLOTHING REQUIRED This symbol indicates that you should wear a work clothing when performing a task or else physical injury could or may occur ELECTRICAL SHOCK HAZARD This symbol indicates that an electrical shock hazard could or may occur EXPLOSION HAZARD This symbol indicates an explosion hazard This symbol indicates this risk could or may cause physical injury FIRE HAZARD This symb...

Page 17: ...lity Failing to carefully read the instructions could or may put you at risk for a physical injury TOXIC SUBSTANCES HAZARD This symbol indicates that exposure to a toxic substance will could or may cause personal injury or death This is the general warning symbol that the ISO 3864 2 standard refers to as the general warning signal to prevent personal injury It is a triangle with an exclamation mar...

Page 18: ...he user must refer to the manual for specific WARNING or CAUTION information to avoid personal injury or damage to the product Caution risk of electric shock Caution hot surface Caution biohazard In position of a bistable push control Out position of a bistable push control Jack socket Symbol in compliance to the Directive 2002 96 EC on Waste Electrical and Electronic Equipment WEEE placed on the ...

Page 19: ... This chapter provides a basic overview of the features and options of the AI 3000 AS 3000 II It also describes the available instrument configurations Contents The AI 3000 AS 3000 II Sampling System Configurations of the 3000 Series Automatic Sampling System Technical Specifications ...

Page 20: ... The AI 3000 AS 3000 II Sampling System The 3000 Series Automatic Sampling System available as AI 3000 or AS 3000 consists of the following parts as respectively shown in Figure 1 and Figure 2 Figure 1 AI 3000 Auto injector Turret Base Safety Door Injection Unit Centering Plate Washing and Waste Tray 8 position Sample Tray ...

Page 21: ...s the structure bearing the sampling unit components Turret It consists of a vertical moving structure that accommodates the injection unit and accomplishes the necessary movements to carry out the three main operating steps sample drawing injection syringe washing The turret is provided with a safety door allowing access to the syringe Turret Safety Door Injection Unit Centering Plate Washing and...

Page 22: ...le vials Each position is numbered to make the sample identification easy The following options are available AI 3000 Auto injector sample tray consisting of an 8 position fixed support AS 3000 Auto sampler ample holding assembly consisting of a 105 position turnable mobile tray mounted on a dedicated support plate Sampler Support It constitutes the supporting base allowing to install the autoinje...

Page 23: ...BKF TRACE GC Ultra S SL PTV PKD PPKD FOCUS GC S SL The autoinjector sampler is available in the following options AI 3000 Auto injector Configuration AS 3000 Auto sampler Configuration Gemini Configuration AI 3000 Auto injector Configuration In this configuration the 8 position sample tray is used See Figure 3 Figure 3 AI 3000 Auto injector Configuration As a function of the configuration of insta...

Page 24: ...rt will be introduced into the relevant housing on the base of the sampling unit Gemini Configuration Consists of the installation of two AI 3000 AS 3000 II on the same GC With the TRACE 1300 TRACE 1310 the samplers are installed on the right side one on the front injector module and the other on the back injector module With the TRACE GC Ultra one sampler is installed on the left side of the GC a...

Page 25: ...he installation of the AI 3000 AS 3000 II requires a dedicated metal support on the base of the sampler support Figure 6 Left Configuration Gemini Configuration Two AI 3000 AS 3000 II are installed on the same TRACE 1300 TRACE 1310 or TRACE GC Ultra by using their own dedicated supports Refer to Sampling System Support on page 23 Note The right configuration is the only one possible on the TRACE 1...

Page 26: ... 10 μL syringe standard 10 μL syringe for Merlin Microseal Valve optional 5 μL syringe optional 0 5 μL syringe for Nano volumes injections optional Volumes programming μl 0 to 5 μL 10 μL syringe with increments of 0 1 μL 0 to 2 5 μL 5 μL syringe with increments of 0 1 μL 0 to 0 5 μL 0 5 μL syringe with increments of 0 01 μL Programmability Remote External interface Serial line RS 232 Power supply ...

Page 27: ...ing Manual 9 2 Sampling Unit This chapter provides a description of the components of the sampling unit of the AI 3000 AS 3000 II Contents Base Turret Injection Assembly Washing and Waste Tray Rear of the Sampling Unit Sample Trays Centering Plate Status LED ...

Page 28: ...for solvents vials and waste container On the lower front part there is a LED indicating the instrument status For details refer to Status LED on page 20 Right and Left Side Sections It can accommodate the 8 position sample tray or 105 position sample tray and the centering plate for the injector The position of the above described accessories depends on the configuration of the auto injector auto...

Page 29: ...only for AS 3000 and for the Gemini configuration Turret The turret consists of a moving structure pivoted on a hub The rotating movements are accomplished by a motor located on the base An optical sensor determines the turret travel ends The front side is provided with a safety door allowing access to the injection assembly The door opening immediately cuts off the power supply to the sampling sy...

Page 30: ...ial capture device and the syringe See Figure 9 Figure 9 Injection Assembly The vertical movements of the injection device are controlled by motors housed in the turret The travel ends of the movable parts is defined by a series of sensors Inside the turret two electronic boards are also present one for the motors control and one for the instrument functions control respectively 5 Safety Door Inje...

Page 31: ...dge the vial presence to acknowledge the injector presence Figure 10 Vial Capture Device Syringe The standard syringe has 10 μL capacity with 50 mm needle length A thorough washing of the syringe can be done thanks to the possibility of using up to four solvents See Washing and Waste Tray To install or replace the syringe please refer to the Chapter 3 Installation and to the Chapter 5 Maintenance ...

Page 32: ...ng functions Sample drawing the turret positions itself on the selected vial housed in the sample tray to allow the syringe to draw the sample it contains Sample injection the turret positions itself on the injector to perform the sample injection Syringe washing the turret positions itself on the solvents vials for the syringe washing The solvent is automatically withdrawn and discharged into the...

Page 33: ...njection The washing solvent is then collected in the waste container The four vials containing solvents for the syringe washing are introduced into the appropriate seats A B and C D on the left and on the right of the waste container respectively marked WASTE Rear of the Sampling Unit The rear side of the sampling unit includes the following components See Figure 13 Figure 13 Rear of the Sampling...

Page 34: ...on through the proper cable Sample Trays The AI 3000 AS 3000 II can accept two types of sample trays which are accommodated in one of the two side housings on the base The type of sample tray accessory installed on the instrument 8 or 105 positions is automatically acknowledged by the autoinjector sampler when switched on and it determines the instrument configuration AI 3000 uses the 8 position s...

Page 35: ...40 are placed into the external row Vials 41 to 80 are placed into the middle row Vials 81 to 105 are placed into the internal row Vials Vials of 0 3 and 2 mL capacity can be used The 0 3 mL vial is exactly the same as the 2 mL vial provided inside with a 0 3 mL calibrated insert Supporting Plate Hub 105 position Sample Tray CAUTION Vials must be accurately closed using appropriate septa and ring ...

Page 36: ...0 The centering plate is provided with two circular guides to center the sampling unit on the PTV and SSL injector nuts To center the sampling unit on the SSL injector nut use the circular guide for SSL only See B of Figure 16 To center the sampling unit on the PTV injector nut use the circular guide for PTV only See A of Figure 17 Figure 16 Circular Guides for SSL CAUTION The circular guide for S...

Page 37: ...C according to the injector mounted A standard centering plate to center the sampling unit on the SSL PKD and PPKD injector nut This centering plate is used also with the FOCUS GC A centering plate to center the sampling unit on the PTV injector nut It is recognized by a circular slot present on the circular guide This centering plate is not used with the FOCUS GC Figure 18 Centering Plate for TRA...

Page 38: ... seconds ON 0 5 seconds OFF 0 5 seconds ON indicates that the start signal is received Five consecutive beep 1 second with interval of 0 5 seconds OFF in case of error condition Table 2 Status LED Conditions Condition LED St by Lit with continuous green light Position zeroing sample preparation washing injection Intermittent green light 0 5 seconds ON 0 5 seconds OFF Run waiting GC Ready Intermitt...

Page 39: ...ringe and the electrical connections with the different units of the gas chromatographic system Contents Who Performs the Installation Electrical Requirements How to Lift and Carry the Sampling Unit Sampling System Support Installing the Sampling System Support on the TRACE 1300 TRACE 1310 Installing the Sampling System Support on the TRACE GC Ultra Installing the Sampling System Support on the FO...

Page 40: ... supplied as indicated in the Technical Specifications on page 8 How to Lift and Carry the Sampling Unit The AI 3000 AS 3000 II must be lifted and carried by hand See Figure 20 Figure 20 How to lift and carry the Sampling Unit CAUTION The power line and the connections between the instruments must maintain good electrical grounding Poor grounding represents a danger for the operator and may seriou...

Page 41: ...e GC 2 The movable top plate is mounted over the bottom plate and it is provided with a guide pivot for the accommodation and centering of the sampling unit The movable top plate can slides over the bottom plate through a sliding slot and a retainer This allows moving away the injection device of the sampling unit from the injector module when the latter will require maintenance To use the proper ...

Page 42: ...t be oriented accordingly before fixing it on the GC See Figure 21 and Figure 22 Figure 21 Support Oriented for the Installation on the Front Injector Module Figure 22 Support Oriented for the Installation on the Back Injector Module Guide Pivot Retainer Sliding Slot L shape Bottom Plate Oriented on Front Plates Locking Screw Top Plate Guide Pivot Retainer Sliding Slot Top Plate Plates Locking Scr...

Page 43: ...3 Figure 23 Support for the Installation in Gemini Configuration Support for the Installation When a TSQ Quantum Mass Spectrometer is Coupled with the GC This configuration uses a dedicated double bottom plate and two top plates See Figure 24 Figure 24 Support for the Installation When a TSQ Quantum is Coupled with the GC Double Bottom Plate Retainer Sliding Slot Guide Pivot Top Plate Double Botto...

Page 44: ... AI 3000 AS 3000 II on the TRACE GC Ultra The AI 3000 AS 3000 II is installed on the right or left side of the TRACE GC Ultra Installation on the Right Side When installing the AI 3000 AS 3000 II on the TRACE GC Ultra right side the support is directly mounted on the GC top panel previous installation of the spacer inside the GC See Figure 25 Installation on the Left Side When installing the AI 30...

Page 45: ...cientific AI 3000 AS 3000 Sampling System Operating Manual 27 Figure 26 Bracket for the Installation of the AI 3000 AS 3000 II Support 1 3 3 3 2 2 2 4 1 Support Bracket 2 Holes on the TRACE GC Ultra Top Cover 3 Fixing Screws 4 Sampling Unit Support ...

Page 46: ... screws In the middle the guide pivot allows the accommodation and centering of the sampling unit Figure 27 Support for the Installation of the AI 3000 AS 3000 II on the FOCUS GC Note The FOCUS GC MS Version may accept the AI 3000 AS 3000 II also on the left side Please refer to Assembling of the Sampling System Support on FOCUS GC MS Version on page 46 To reduce the FOCUS GC working area the AS 3...

Page 47: ...n For the installation of the AI 3000 AS 3000 II please refer to Installing the Sampling System Support on the TRACE 1300 TRACE 1310 on page 30 Installing the Sampling System Support on the TRACE GC Ultra on page 43 Installing the Sampling System Support on the FOCUS GC on page 46 Installation of the AI 3000 AS 3000 II on the GC on page 49 ...

Page 48: ...300 TRACE 1310 Installation Holes for the AI 3000 AS 3000 II on the Front Injector Module See Assembling of the Sampling System Support on the Back Injector Module Installation Holes for the AI 3000 AS 3000 II on the Back Injector Module See Assembling of the Sampling System Support on the Front Injector Module Installation Holes for Two AI 3000 AS 3000 in Gemini Configuration See Assembling of th...

Page 49: ... Figure 29 Assembling on the Front Injector Module 1 1 Remove from the GC top cover the three plastic caps covering the corresponding fixing holes See A B and C 2 Orient the L shape bottom plate to align the three installation holes to the corresponding fixing holes located on the GC top 3 Insert the provided fixing screws into the holes present on the L shape bottom plate Note In comparison with ...

Page 50: ... 5 Tighten the three fixing screws 6 If not already done mount and fix the guide pivot on the top plate by using the proper fixing screw 7 Place the top plate over the L shape bottom plate 8 Guide the retainer and its fixing screw into the sliding slot present on the top plate 9 Align the retainer fixing screw into the corresponding fixing hole located on the L shape bottom plate 10 Tighten the re...

Page 51: ... 3000 AS 3000 Sampling System Operating Manual 33 Note Removing the plates locking screw the top plate is free to move outwards See the figure below This allows the sampling unit when installed to be moved away from the injector module See Moving Away the Sampling Unit from the Injector Module on page 80 ...

Page 52: ...Figure 31 Assembling on the Back Injector Module 1 1 Remove from the GC top cover the three plastic caps covering the corresponding fixing holes See A B and C 2 Orient the L shape bottom plate to align the three installation holes to the corresponding fixing holes located on the GC top 3 Insert the provided fixing screws into the holes present on the L shape bottom plate Note In comparison with th...

Page 53: ... 5 Tighten the three fixing screws 6 If not already done mount and fix the guide pivot on the top plate by using the proper fixing screw 7 Place the top plate over the L shape bottom plate 8 Guide the retainer and its fixing screw into the sliding slot present on the top plate 9 Align the retainer fixing screw into the corresponding fixing hole located on the L shape bottom plate 10 Tighten the re...

Page 54: ...ampling System Operating Manual Thermo Scientific Note Removing the plates locking screw the top plate is free to move outwards See the figure below This allows the sampling unit when installed to be moved away from the injector module See Moving Away the Sampling Unit from the Injector Module on page 80 ...

Page 55: ...f how to mount the sampling system support on the TRACE 1300 TRACE 1310 for the installation of two AI 3000 AS 3000 II sampling systems in Gemini configuration See Figure 33 and Figure 34 Figure 33 Assembling In Gemini Configuration 1 1 Remove from the GC top cover the five plastic caps covering the corresponding fixing holes See A B and C 2 Insert the provided fixing screws into the three holes p...

Page 56: ... already done mount and fix the guide pivot on each top plate by using the proper fixing screw 6 Place the two top plates over the double bottom plate 7 Guide each retainer and its fixing screw into the sliding slot present on each top plate 8 Align each retainer fixing screw into the corresponding fixing hole located on the double bottom plate 9 Tighten each retainer fixing screw to block the cor...

Page 57: ...Sampling System Operating Manual 39 Note Loosening the screw that secure each retainer the corresponding top plate are free to move outwards See the figure below This allows the sampling unit when installed to be moved away from the injector module See Moving Away the Sampling Unit from the Injector Module on page 80 ...

Page 58: ...ow to mount the sampling system support on the TRACE 1300 TRACE 1310 for the installation of one or two AI 3000 AS 3000 II sampling systems when a TSQ Quantum mass spectrometer is coupled with the GC See Figure 33 and Figure 35 Figure 35 Assembling 1 1 Remove from the GC top cover the five plastic caps covering the corresponding fixing holes See A B and C 2 Insert the provided fixing screws into t...

Page 59: ...e mount and fix the guide pivot on each top plate by using the proper fixing screw 6 Place the two top plates over the double bottom plate 7 Guide each retainer and its fixing screw into the sliding slot present on each top plate 8 Align each retainer fixing screw into the corresponding fixing hole located on the double bottom plate 9 Tighten each retainer fixing screw to block the corresponding u...

Page 60: ... Operating Manual Thermo Scientific Note Loosening the screw that secure each retainer the corresponding top plate are free to move outwards See the figure below This allows the sampling unit when installed to be moved away from the injector module See Moving Away the Sampling Unit from the Injector Module on page 82 ...

Page 61: ...e sampling system support the spacer should be installed inside the GC then assemble the sampling system support on the GC according to the required configuration See Installing the Spacer Inside the GC See Assembling of the Sampling System Support on the GC Right Side See Assembling of the Sampling System Support on the GC Left Side Installing the Spacer Inside the GC To installing the spacer sho...

Page 62: ...t configuration i e on the right side of the TRACE GC Ultra 1 Remove from the GC top cover the two plastic caps covering the corresponding fixing holes 2 Insert into each slot present on the support the provided fixing screw 3 Insert each screw into the relevant spacer paying attention to keep its largest surface turned towards the support base 4 Holding the spacers in position with your hands res...

Page 63: ...o the relevant spacer paying attention to keep its largest surface turned towards the support base 5 Holding the spacers in position with your hands rest the support on the bracket with their flat side towards the inside 6 Guide the two fixing screws located on the external spacers into the corresponding fixing holes located on the bracket 7 Loosely tighten the screws ...

Page 64: ...the right side of the FOCUS GC 1 Remove from the GC upper cover the two plastic caps covering the corresponding fixing holes 2 Insert into each slot present on the support the provided fixing screw 3 Guide the two fixing screws into the corresponding fixing holes located on the GC upper panel 4 Loosely tighten the screws Assembling of the Sampling System Support on FOCUS GC MS Version The installa...

Page 65: ...xing screw into each slot present on the support 3 Guide the two fixing screws into the corresponding fixing holes located on the GC upper panel See Figure 40 Figure 40 Sampling System Support on the FOCUS GC MS Version 3 4 Loosely tighten the screws Space Saving Installation This type of installation is suggested to reduce the working area of your FOCUS GC equipped with the AS 3000 This is applic...

Page 66: ...om the GC upper cover the two plastic caps covering the corresponding fixing holes 2 Insert into each slot present on the support the provided fixing screw 3 Guide the two fixing screws into the corresponding fixing holes located on the GC upper panel 4 Loosely tighten the screws A B 1 2 3 2 1 1 3 4 4 A Conventional Installation B Space Saving Installation 1 Fixing Screws 2 Sampling System Support...

Page 67: ...ctrical Connections Instrument Start up Installation of the Sampling Unit Proceed as follows 1 Lift the sampling unit see Figure 20 on page 22 and insert it into the guide pivot located on the sampling system support See Figure 42 Figure 42 Installation of the Sampling Unit 2 Insert the proper centering plate for TRACE GC Ultra or TRACE 1300 TRACE 1310 see Figure 43 into its seat of the sampling u...

Page 68: ... AI 3000 insert the 8 position sample tray into the appropriate housing of the sampling unit base See Figure 44 Figure 44 8 position Sample Tray AS 3000 insert the dedicated support plate into its appropriate housing of the sampling unit base Place the 105 position sample tray on the hub located on the support The system will automatically recognize the sample tray at the instrument power on See F...

Page 69: ...m needle length 5 and 0 5 μL optional syringes are optionally available 1 Open the safety door of the turret 2 Insert the syringe needle into the vial capture device See Figure 46 Figure 46 Syringe Installation 1 3 Accommodate the syringe body into its seat paying attention to insert simultaneously the flange and head of the syringe plunger into their relevant guides 4 Lock the syringe by turning ...

Page 70: ...ements on page 22 have been fulfilled This section provides the instruction to connect the AI 3000 AS 3000 II in standard o Gemini configuration to a TRACE 1300 TRACE 1310 TRACE GC Ultra or FOCUS GC Refer to Standard Configuration Gemini Configuration Standard Configuration Proceed as follows 1 By using the cable provided connect the 6 pin female connector marked GC located on the sampling unit ba...

Page 71: ...sampling unit of the sampler a to the 4 pin male connector marked TWIN SYNC on the rear of the sampling unit of the sampler b 2 By using the Y shape cable provided connect a the 6 pin female connector marked GC located on the sampling unit back side and the connector located on the rear panel of the GC marked AUTOSAMPLER HANDSHAKE in the case of TRACE 1300 TRACE 1310 SAMPLER SIGNAL in case of TRAC...

Page 72: ...nstrument Start up Proceed as follows 1 Plug in the Vdc power cable of the external portable power supply into the jack marked 24 Vdc located on the sampling unit back side 2 Connect the power cord of the external power supply to the mains outlet The AI 3000 AS 3000 II will automatically run the self testing routine during which the following automatic checks and settings are carried out alignment...

Page 73: ...apter provides the information to control the AI 3000 AS 3000 II from the computer or the GC The chapter contains also the working procedures with different injectors Contents Control from PC Control Through the TRACE GC Ultra Control Through the FOCUS GC Guidelines for Programming with Different Injectors ...

Page 74: ...a function of the type of sample tray installed Figure 49 AI 3000 AS 3000 II Autosampler Page When in Gemini configuration the user interface controls both the samplers The parameters set here will be send to both the samplers as they were a single ones The hardware must be the same for both the samplers such as type of sampler tray and samples numbering IMPORTANT Before creating an autosampler me...

Page 75: ...he range is 0 0 2 5 μl and for a 10 μl syringe 0 0 5 0 μl Plunger Strokes Select the number of strokes of the plunger used to eliminate air bubbles The range is 1 15 Viscous Sample Select the check box to indicate that the sample is viscous Clearing the check box implements a fast aspiration speed while a selected check box leads to a slow one Sampling Depth Choose the position of the needle in th...

Page 76: ... 7 for details Post injection Dwell Time This is the time that the needle is held in the inlet after the injection Enter a value in the range 0 63 s Table 4 Injection Parameters Sheet 2 of 2 Parameter Description Table 5 Washer Parameters Parameter Description Pre Injection These parameters are applied to pre injection washing with a solvent chosen from the vials in the Washing and Waste tray See ...

Page 77: ...he computer memory Open Recent It allows to retrieve the last 5 saved analytical methods Save Save as This function allows to save an analytical method on the hard disk With the command Save as the method can be saved with a new name Load From Default This function allows to upload a default analytical method Save As Default This function allows to save the current analytical method as a default m...

Page 78: ...th the operator s name as well as the date and time of the modification Using the function Copy it is possible to copy part or all the log file contents into Windows and then transfer information to other programs if required Command Menu This menu gives access to the following functions Start Clean Cycle It allows to start a washing cycle Inject Single Sample It allows to start the injection of a...

Page 79: ...king positions AI 3000 AS 3000 II ready to inject sample in vial n but waiting GC Ready AI 3000 AS 3000 II is preparing the sample in vial n AI 3000 AS 3000 II has injected the sample in vial n The AI 3000 AS 3000 II front door is open all operations are paused Error Messages The error messages are listed below AI 3000 AS 3000 II error solvent not found AI 3000 AS 3000 II position error cannot fin...

Page 80: ...witched on the presence of the autoinjector sampler and its configuration is automatically acknowledged The type of configuration is displayed by pressing CONFIG and selecting AUTOSAMPLER The following non editable message is displayed according to the AI 3000 AS 3000 II installed for the AS 3000 or for the AI 3000 Note It is not possible to control the 0 5 μL syringe through the GC keypad but onl...

Page 81: ...to eliminate air bubbles forming during the sample withdrawal Pre wash solvent A B C D A B C D This parameter allows to select the vial or combination of two vials containing the washing solvent Press Mode Type to select the solvent vial to be used Pre wash cycles 0 15 This parameter allows to set the number of syringe pre washings with the selected solvent Post wash solvent A B C D A B C D This p...

Page 82: ... This parameter defines the speed at which the sample is drawn from the vial as a function of the sample viscosity Select No default value if the sample has low viscosity Select Yes if the sample has high viscosity Sampl depth Bottom Center selectable in the submenu This parameter determines the penetration depth of the syringe needle into the vial Press Mode Type or Enter to enter the submenu Sel...

Page 83: ...ble 10 Control Through the TRACE GC Ultra When No Vial Abort Menu Menu Comments ON MISSING AS VIAL This line shows the menu title Skip to next If this function is selected the AI 3000 AS 3000 II skips a missing vial and goes to the next one The sample sequence and the sample table are not affected Abort sequence If this function is selected the sequence is aborted after a missing vial ...

Page 84: ... of samples Refer to paragraph Sequence of Samples with the AS 3000 on page 68 Configuring the AI 3000 AS 3000 II When the GC is switched on the presence and type of sampling system installed is automatically acknowledged To configure the AI 3000 AS 3000 II 1 In Main Menu move the cursor until Others is displayed then press Enter 2 Scroll to select Configuration then press Enter 3 Scroll to Autosa...

Page 85: ...s with the sample Plunger strokes 0 15 This parameter allows to set the number of plunger strokes to eliminate air bubbles forming during the sample withdrawal Pre wash solvent A B C D A B C D This parameter allows to select the vial or combination of two vials containing the washing solvent Pre wash cycles 0 15 This parameter allows to set the number of syringe pre washings with the selected solv...

Page 86: ...needle into the injector If Standard defualt value is selected the needle goes down to the maximum allowed depth If Minimum is selected the needle enters the injector and stops immediately beyond the septum Cold Needle Technique Pre dwell time 0 63 sec This parameter specifies how long the syringe needle must remain in the injector before injection Hot Needle Technique Post dwell time 0 63 sec Thi...

Page 87: ...h the TRACE GC Ultra When No Vial Abort Menu Menu Comments ON MISSING AS VIAL This line shows the menu title Skip to next If this function is selected the AI 3000 AS 3000 II skips a missing vial and goes to the next one The sample sequence and the sample table are not affected Abort sequence If this function is selected the sequence is aborted after a missing vial Table 12 Control Through the FOCU...

Page 88: ... provide needle heating In the latter the needle penetrates for a minimum depth and for the minimum time allowing to minimize contact of the needle with the hot inlet These two needle insertion modes are conceived to optimize respectively the Hot Needle and the Cold Needle injection techniques Cold needle technique Minimum depth mode prevents boil out from the needle and lets the sample to enter t...

Page 89: ... and a standard split or splitless liner possibly packed with glass wool whenever you inject in Cold Needle mode In summary use the following guideline to choose the best syringe capacity for your injections with the AI 3000 AS 3000 II autosampler See Table 15 for details Table 14 Summary of Injection Parameters and Conditions Mode Technique Column Insertion Liner Type Packing of Liner AI 3000 AS ...

Page 90: ...reached room temperature and by means of the outfit tool unscrew the septum cap then remove it together with the septum holder and its plate 6 Unscrew the liner holder and remove it Use tweezers to gently extract the liner from the injector 7 Insert a little plug of packing material i e deactivated glass wool filling the liner cavity about 5 mm in length Push the plug till it reaches the position ...

Page 91: ...nge needle will not be heated whenever standard or minimum depth is selected or dwell times are set However standard injection mode is advisable since the sample evaporation is faster in the deeper part of the liner Optional 1 2 seconds of dwell times will guarantee stable flow condition after the needle introduction See Table 16 Table 16 PTV Inlet Note In order to guarantee long life performance ...

Page 92: ...lumn the standard insertion depth with 3 5 seconds of Pre dwell time is suggested See Note In order to guarantee long life performance of the Merlin Microseal it is recommended to use syringes with 0 63 mm needle diameter and truncated cone shape on tip Please refer to the spare part catalog for part number Table 17 PKD and PPKD Inlets Injector AI 3000 AS 3000 II Injection Parameters Packed Inject...

Page 93: ...idelines for the maintenance of the AI 3000 AS 3000 II Contents Current Maintenance Emptying of the Waste Container Cleaning of the Sample Tray Accessory Cleaning the Instrument Externally Replacing the Syringe Cleaning the Syringe Preparing a New Syringe Moving Away the Sampling Unit from the Injector Module ...

Page 94: ...t be periodically cleaned Use a water and soap solution or a household non abrasive product Dry using a clean cloth Cleaning the Instrument Externally Normal usage of the AI 3000 AS 3000 II can cause the exterior to get dirty To clean the instrument externally 1 Power off the AI 3000 AS 3000 II 2 Externally clean the instrument with a soap and water solution or with a household non abrasive produc...

Page 95: ... needle length 5 and 0 5 μL syringes are optionally available This operation is possible with the instrument either on or off 1 Open the safety door of the turret 2 Turn the syringe locking knob by 180 counterclockwise 3 Gently take out the syringe plunger head and flange from their relevant guides then remove the syringe body from its seat 4 Completely take out the syringe paying attention while ...

Page 96: ...ections see Automated Cleaning on page 78 5 Perform a manual maintenance routine every 1000 injections see Manual Cleaning on page 78 Automated Cleaning The syringe maintenance cycle is always advisable after the injection of pure samples of undiluted mixes of liquids containing solids in suspension or at any rate after long sequence of analyses To perform an automated cleaning cycle proceed as fo...

Page 97: ... the operating sequence Replacing the Syringe on page 77 Preparing a New Syringe This operating procedure is always advisable before using a new syringe with low capacity like a 5 or 0 5 μL or a new 10 μL syringe with Teflon tip plunger The following procedure will allow to obtain the proper lubrication of the plunger To prepare a new syringe proceed as follows 1 Fill a 4 mL vial with fresh solven...

Page 98: ... the sampling unit base paying attention that the guide hole present on the arm of the centering plate correctly fits the injector nut Gemini Configuration 1 Power off the AI 3000 AS 3000 II 2 Remove the centering plate from the injector nut and from its seat on the sampling unit base 3 Remove the sample tray from the sampling unit base 4 Undo that screw that secure the retainer The top plate is n...

Page 99: ...ic prefixes symbols and abbreviations A A ampere ac alternating current ADC analog to digital converter B b bit B byte 8 b baud rate data transmission speed in events per second C C Carbon C Celsius CIP Carriage and Insurance Paid To cm centimeter CPU central processing unit of a computer Ctrl control key of the keyboard A B C D E F G H I J K L M N O P Q R S T V V W X Y Z ...

Page 100: ...tromagnetic compatibility ESD electrostatic discharge F ºF Fahrenheit FOB Free on board FSE Field Service Engineer ft foot G g gram GC gas chromatography gas chromatograph GND electrical ground H h height h hour harmonic distortion A high frequency disturbance that appears as distortion of the fundamental sine wave HV high voltage Hz hertz cycles per second ...

Page 101: ...ee transient IEC International Electrotechnical Commission in inch I O input output K k kilo 103 or 1024 K Kelvin kg kilogram kPa kilopascal L l length L liter LAN Local Area Network lb pound LED light emitting diode M m meter or milli 10 3 M mega 106 μ micro 10 6 min minute mL milliliter mm millimeter ...

Page 102: ...tor psi pounds per square inch PTV Programmable Temperature Vaporizing Injector R RAM random access memory Return Return key on the keyboard RF radio frequency ROM read only memory RS 232 industry standard for serial communication S s second sag See surge slow average A gradual long term change in average RMS voltage level with typical duration greater than 2 s source current The current needed to...

Page 103: ...A brief voltage surge of up to several thousand volts with a duration of less than 50 μs V V volt V ac volts alternating current V dc volts direct current VGA Video Graphics Array W w width W Watt When a unit of measure has a quotient e g Celsius degrees per minute or grams per liter this can be written as negative exponent instead of the denominator For example C min 1 instead of C min g L 1 inst...

Page 104: ...A 86 AI 3000 AS 3000 Sampling System Operating Manual Thermo Scientific ...

Page 105: ... Syringe 78 Command Menu 60 Configuration AI 3000 II Auto injector 5 AS 3000 II Auto sampler 6 Gemini 6 Left 7 Right 7 Configuration through the TRACE GC Ultra 62 Extended Control Menu 64 Menu 63 When No Vial Abort Menu 65 Configurations 5 Contacting Us xv Control from PC 56 Control Through the FOCUS GC 66 Control Through the TRACE GC Ultra 62 D Dimensions 8 E Edit Menu 60 Electrical Connections 5...

Page 106: ...ges 60 N Nano Volumes Injections in S SL Inlets 71 P Packing a SSL Liner 72 Parameters Injection 57 Sampling 57 Washes 58 PKD Inlet 74 Plunger strokes 63 67 Post dweel time 68 Post wash cycles 63 67 Post wash solvent 63 67 Power supply rating 8 PPKD Inlet 74 Pre dweel time 68 Pre wash cycles 63 67 Pre wash solvent 63 67 Programmability 8 Programming with Different Injectors 70 Nano Volumes Injecti...

Page 107: ...away from the Injector Module 80 Rear 15 Rear side 15 Sample Trays 16 Status LED 20 Turret 11 Vial Capture Device 13 Washing Tray 15 Waste Tray 15 15 Sequence of Samples with the AS 3000 Autosampler 68 Signal Words xvi SSL Inlet 70 Start sequence 69 Start up 54 Status LED 20 Buzzer 20 Condition 20 Stop Sequence 69 Support for the Installation on the Front Back Injector Module 24 Supporto del campi...

Page 108: ...90 AI 3000 AS 3000 Sampling System Operating Manual Thermo Scientific Index W ...

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