2
Accela 600 Pump or Accela 1250 Pump Installation
Solvent Line Connections
28
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
Connecting the Solvent Reservoir Bottles to the Degassing Unit
The Accela 600 Pump Inlet Tubing Kit, which ships with both the Accela 600 Pump and the
Accela 1250 Pump, contains four preassembled solvent reservoir lines. Each solvent line
terminates with a color-coded Super Flangeless fitting at one end and a stainless steel,
20 μm particle size sinker frit at the other end.
Super Flangeless™ fittings consist of three components: a nut, a ferrule, and a stainless steel
compression ring (see
Figure 14.
Super Flangeless fitting components (with color-coded nuts)
The compression ring has two sides: a flat side and an angled side (see
). The flat side
of the ring faces the nut, and the angled side of the ring faces the narrow end of the ferrule.
CAUTION
Use appropriate solvents with your Accela LC system:
• Use HPLC- or LCMS-grade solvents that are free of particulate matter.
• Do not use solvents containing Freon and perfluorinated solvents, such as Fluorinert
and Fomblin perfluoro polyether solvents. They adversely affect the Teflon AF
degassing membrane.
CAUTION
To prevent personal injury, observe good laboratory practice when handling
solvents, changing tube lines, or both. Consult the pertinent material safety data sheets for
the solvents used for HPLC analysis.
Nut
Compression ring
Ferrule
