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Chapter 6

HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE

6-18

MR23i

Thermo Fisher Scientific

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Summary of Contents for 11174729

Page 1: ...P N 50116911 1 Analyze Detect Measure Control Instruction Manual MR 23i ...

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Page 3: ...PN 50116911 1 Issued 06 08 Instruction Manual Jouan MR 23i ...

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Page 5: ...2 4 ROTOR INSTALLATION 2 4 ROTOR REMOVAL 2 4 Chapter 3 SPECIFICATIONS 3 1 DIMENSIONS AND WEIGHT 3 1 ELECTRICAL SPECIFICATIONS 3 1 PERFORMANCE 3 1 Acceleration rate control 3 2 Braking rate control 3 2 Rotor selection table 3 3 Chapter 4 OPERATING PRINCIPALES 4 1 DRIVE SYSTEM 4 1 SAFETY INTERLOCK SYSTEM 4 1 IMBALANCE DETECTION SYSTEM 4 1 REFRIGERATION SYSTEM 4 2 INTEGRATION 4 2 Chapter 5 INSTRUCTIO...

Page 6: ...OPERATIONAL LIMITATIONS 6 15 ELECTRICAL 6 16 IMPROPER USE 6 16 BIOLOGICAL RISKS 6 16 Chapter 7 SERVICING AND PREVENTATIVE MAINTENANCE 7 1 CLEANING 7 1 PERIODIC PREVENTATIVE MAINTENANCE 7 1 CIRCUIT BREAKER 7 1 Annex A GUARANTEE TERMS A 1 ...

Page 7: ...th a CFC free fluid The refrigerant fluid circulates around the chamber to achieve maximum efficiency RELATIVE CENTRIFUGAL FORCE Suspended solids are separated by the Relative Centrifugal Force RCF applied to the sample The effective force increases with the square of the speed of rotation and the distance from the axis of rotation The following formulae permit the calculation of primary parame te...

Page 8: ...ieved at a different speed To determine actual speed required to achieve a different g at the same radius To determine actual speed required to achieve the same g at a diffe rent radius RCF xg 1 118RN2 Nx x1 118xR Speed rpm 946 RCF R RCF R x946 Radius mm RCF 1 118N 2 Nx x1 118 M RCF MR RCF2 RCF1 N2 N1 2 Nx x1 118 M RCF MR N2 N1 RCF2 RCF1 RCF2 RCF1 xN1 N2 N1 R1 R2 R1 R2 xN1 ...

Page 9: ...ted without its pallet for example on a staircase professio nal handling assistance is required UNPACKING Delivery should include the following items 1 Centrifuge 1 Tool kit 1 User s manual Unscrew the 4 wing nuts situated at the base of the packaging Lift off the upper part of the packaging With the aid of a Philips headed screwdriver unscrew the holding screw from each side of the instrument IMP...

Page 10: ...0 C Maximum relative humidity of 80 for temperatures up to 22 C Maximum altitude 2000 m Maximum performance is assured across the following tempera ture range 15 C to 25 C POSITIONING Place the centrifuge on a bench top able to support its weight of 85 kg and vibrations in a clean noncorrosive environment Posi tion the centrifuge Leave a 30 cm space free on each side of the machine and behind LID ...

Page 11: ...ocking must only be done by someone informed of the danger and of the necessary precautions to be undertaken If the machine has stoppped because of a mains power cut the rotor could still be rotating Wait 10 minutes before opening the lid In spite of the absence of noise the rotor could still be rotating when you need to open the lid manually Even in the case of a mains power cut always set the ma...

Page 12: ...e rotor lid if any screws on easily and that its thread is lightly lubricated SAMPLE LOADING The contents of each rotor pocket including sample tube cap and adaptor where used must be of the same weight as the one dia metrically opposite Maximum weight difference accepted CAUTION Imbalance of the rotor may cause major damage to the rotor and centrifuge Do not attempt to introduce liquids into roto...

Page 13: ...ULTING FROM BROKEN OR LEAKING TUBES AND BOTTLES ROTOR REMOVAL For rotors with accessible centres press a thumb against the spring locking device and lift with the fingers while pulling with the other hand supporting the rotor For rotors with inaccessible centres press the unlocking device stored beneath the centrifuge lid into the centre of the rotor and then lift out the rotor using both hands Re...

Page 14: ...Chapter 2 INSTALLATION PROCEDURE 2 6 MR23i Thermo Fisher Scientific ...

Page 15: ...174731 230 VAC 60 Hz Max power 1 300 VA Average power 1 000 VA Refrigeration 500 W Max speed Swing out 4 400 rpm Angle 23 000 rpm Max RCF 8 x 50 ml Swing out 3 074 x g Angle 36 668 x g Max capacity Swing out 4 x 200 ml Angle 6 x 100 ml Microprocessor controlled Display Graphic screen 240 x 128 pixels Memory size 32 programs Program protection Individual password Self diagnostic function Speed Rang...

Page 16: ...es into effect Above 2 000 rpm there would be no gain in quality of separation if a slower rate of braking could be applied Range 10 rates 0 to 9 Duration 15 from maximum speed to 0 rpm slope 9 1 Integrator Range 100 to 281 x 1012 m rd2 s Step 1 m rd2 s Acceleration rates 10 Braking rates 10 Temperature Range 8 C to 40 C Step 1 C Accuracy 1 5 C Typical performance 5 C at 4400 rpm 3074 x g 4 x 200 ...

Page 17: ... 11174563 1 Rack 1 x 600 µl ø 6 90 11174562 1 Rack 32 x 250 400 µl ø 6 90 11174704 AM 2 23 Unsealed angle rotor 12 x 1 5 2 ml 11 x 39 62 23000 36668 45 11174728 AM 2 18 Sealed angle rotor 24 x 1 5 2 ml 11 x 39 84 18750 33016 45 11174706 AM 2 19 Sealable angle rotor 28 x 1 5 2 ml 11 x 39 73 19200 30086 45 63 19200 25965 45 11174722 Trans sealing lid 11177523 FAJM 2 15C Sealable angle rotor 48 x 1 5...

Page 18: ... 38 ml 25 5 x 92 88 13000 16627 28 11174716 Set of 6 adaptors 1 x 15 ml con 17 x 122 93 13000 17572 28 11174717 Set of 6 adaptors 2 x 10 ml 16 x 80 83 13000 15682 28 11174718 Set of 6 adaptors 4 x 1 5 2 ml 11 x 39 79 13000 14926 28 11174711 SWM 180 5 Swing out rotor 4 x 200 ml 142 4400 3074 90 11174720 Set of 4 standard buckets 180 ml Ø 57 142 4400 3074 90 11174721 Set of 4 sealed buckets 180 ml Ø...

Page 19: ...ses friction with the rotor which leads to an increase in the acceleration time up to 8 minutes If the chamber was at 4 C before the run the AM50C 13 will never reach 13 000 rpm 11174533 Set of 4 inserts 5 x Z5 5 ml 17 x 60 142 4400 3074 90 11174504 Set of 4 inserts 5 x 5 7 ml Ø 13 5 142 4400 3074 90 11174505 Set of 4 inserts 9 x 5 6 ml Ø 13 5 142 4400 3074 90 11174577 Set of 4 inserts 12 x 5 ml o...

Page 20: ...an 10 C but temperature setting at 4 C and speed at 13 000 rpm chamber temperature will never decrease When running at ultimate conditions 13 000 rpm and temperature below 8 C cycling speed variations can be observed of about 200 rpm which does not affect the efficiency of centrifugation ...

Page 21: ...r R C F and increases the speed of separation of the sub stances SAFETY INTERLOCK SYSTEM The Jouan MR23i is equipped with an interlock system that pre vents opening of the centrifuge lid when the rotor is spinning The centrifuge will not operate until the lid is closed and latched in place The lid remains latched until the rotor stops spinning When the rotor has stopped spinning the stand by scree...

Page 22: ...t The operator can use a temperature compensation procedure so that the sample temperature can be more accurately controlled allowing for its original temperature Empirical tests by the user will enable him to know what air tempe rature to select compared to the normal temperature of the proto col For example suppose samples at 20 C need to be spun at 4 C It might be found with a protocol temperat...

Page 23: ...1 The centrifugation curve is as follows Theoretically if the centrifuge instantly attains the set point speed and brakes instantly and if the requested speed attained is con stant reproducibility of results is guaranteed from one centrifuga tion to the next It is thus obviously equivalent to centrifuge a sample at 4000 g during 10 minutes as to centrifuge it at 2000 g for 20 minutes or 8000 g for...

Page 24: ...ementary time interval x acceleration applied during this time interval The curve of two successive centrifugations for which the same speed and duration are applied are not identical Indeed according to sample weight temperature atmospheric moisture in the cham ber mains voltage fluctuations and motor wear the acceleration rate as well as constant speed phase may vary When constant speed duration...

Page 25: ...ndicated Press ENTER to select control by integral instead of time and once saved the pro gram will give guaranteed run to run reproducibility for the life of the centrifuge for this protocol Thermo Fisher Scientific makes it easy to use the most accurate control method However there is another very practical advantage You can make major time savings This translates into faster results and or more...

Page 26: ...Chapter 4 OPERATING PRINCIPALES 4 6 MR23i Thermo Fisher Scientific ...

Page 27: ... and more than one key can operate the same function when the name of the function is displayed across a wide area of the screen next to these keys In addition a standard numeric keypad 0 to 9 and CE clear entry enables parameter values to be keyed in directly when required Certain key functions are very common others are found only on a dedicated screen They operate as follows Increases the value...

Page 28: ... values have been entered END PROG Returns to the stand by screen keeping currently selected values in volatile memory ready for use but not in permanent memory for later recall ENTER Returns to a previous screen memorizing all changes in parameter values ESC Returns to a previous screen without memorizing any changes in parameter values EXPAND DISPLAY Switches the display from the basic run scree...

Page 29: ... software SWITCHING ON Immediately after switching on the screen displays Thermo Elec tron Corporation then the stand by screen is displayed showing the number of the last application program used and its basic para meters NONE Selects NO PASSWORD PROTECTION when memorizing an application PRE COOL Starts a predetermined precooling cycle 2000 rpm 4 C 15 min During this cycle all other pro gramming ...

Page 30: ... real temperature and the dynamic rotation symbol are dis played Uniquely during a PRECOOL run it is possible to access all pro gramming facilities The precool run can be halted at any time using the STOP keys STANDARD USE If the program which is currently displayed is the one desired press a START key to run the program Otherwise another program can be recalled by selecting DISPLAY APPLI which fi...

Page 31: ...ure and time are quickly introduced after selecting NEW APPLI on the stand by display to show the simple programming screen Speed is indicated by a flashing arrowhead Use the keypad to write a new speed value if required Move to temperature using Use the keypad to write a new temperature value If required use the to change the sign of the value Move to time using Use the keypad to write a new time...

Page 32: ...the cursor down the list of rotors to that which will be used If an adaptor will be used in the rotor select RADIUS and use the keys to choose the effective radius see accessory table Chapter 3 SPE CIFICATIONS Section Rotor selection table and select ENTER During operation with the dynamic radius arrow moves and the related RCF is changed in real time If the user wishes to modify the acceleration ...

Page 33: ...tion of ENTER will return the user to the simple programming screen but with control by integral instead of time ensuring the highest possible run to run reproducibility see Chapter 4 OPE RATING PRINCIPALES Section INTEGRATION Note This will only be possible if a rotor has already been selected the integral value being linked to the g force which is calculated from the centrifugation radius of the...

Page 34: ...s a special procedure to enable the protection to be overridden This same procedure is used to clear one or more memories to eliminate unwanted applications After SAVE APPLI has been selected the file screen is displayed To eliminate the password protection and erase a program use the and keys to move to the required memory closed file icon and select CE Use and CE to clear any other memories Movi...

Page 35: ...E SEL ECTION then select ENTER Select 1 for ENGLISH or 2 for FRENCH The stand by screen will be displayed in the selected language B Selection of refrigeration stand by Having selected CONFIG use to highlight STAND BY REFRI GERATION ON OFF then select ENTER Choose 1 for active refrigeration during stand by and 2 for refrige ration OFF during stand by C Service A special code reserve SAFETY At any ...

Page 36: ...r case its real value will be displayed and in every case a TIME OUT safety lock will be applied preven ting the user from opening the lid for a set period after the speed reaches zero or at least effects this lock out period in the case of no detected speed MOTOR OVERTEMPERATURE When using the centrifuge in bad conditions e g motor failing a sensor detects an overtemperature in the motor and the ...

Page 37: ...ing of the accessories of the rotor chamber lubrication of the trunnions Centrifuge ware shape material and quality of the vessels must be in accordance with the performance of the centrifuge Load balancing Samples cleaning of the accessories is particularly necessary when using corrosive products in the samples saline acids bases ROTOR AND ACCESSORY PRECAUTIONS CORROSION INFORMATION Thermo Fisher...

Page 38: ...the origin of this corrosion do not necessarily originate from broken tubes for example they could come from Chemical vapours present in the laboratory which are dissolved in the residual humidity in condensed water refrigerated cen trifuges present at the base of the rotor pockets Corrosive liquids originating from overfilled uncapped tubes the liquid overflowing during centrifugation Inserts ada...

Page 39: ...nce of the accessory to centrifugal force As accessories are designed with high levels of safety rupture does not occur as soon as the first microfissures are produced Depending upon the location of the fissure disruption may occur before it reaches the external surface of the accessory The fissure creates a weakness which makes the accessory less and less resi stant to mechanical fatigue The corr...

Page 40: ...ical reaction could take place with the aluminium or magnesium in the rotor CONTAMINATION HAZARDS Our centrifuges are likely to be used in medical research where hazardous substances including radioactive chemicals are fre quently found see also Chapter 6 HAZARDS PRECAUTIONS AND LIMITATIONS OF USE Section BIOLOGICAL RISKS ALWAYS USE THE APPROPRIATE DECONTAMINATION PRO CEDURES WHERE THE ROTOR IS EX...

Page 41: ...lin then dried completely Note FORMALDEHYDE IS TOXIC Gluteraldehyde 2 sold under many brand names such as Cidex and Glutarex requires total immersion for 10 minutes to ensure sterility Thorough rinsing and drying is essential to protect users CAUTION GLUTERALDEHYDE BUILDS UP TO A TOXIC LEVEL IN THE FATTY TISSUES OF THE BODY Phenols are very corrosive and should never be used Radioactive decontamin...

Page 42: ...ly preconditioned by storing in a cold room or refrigerator This tends to advance the onset of corrosion We recommend therefore that such rotors are stored dismantled sealed inside plastic bags from which the air has been evacuated S Satisfactory D Discoloration but OK P Pure chemical OK M Moderate resistance U Unsatisfactory X Explosion risk O No information d Less resistance if T 50 C t Unsatisf...

Page 43: ...Ammonia S O O O O O O O S U S S Sd O O O O O O Ammonium Acetate O O O O O O O O S S S S O O S S S O O Ammonium Carbonate S U S S O S O S S O S S S S S S S O S Ammonium Chloride M O O O O O O O O St O St St O O O O O O O Ammonium Hydroxide 10 O S U O O O O S D U S D S S S S S S S Ammonium Hydroxide conc O U U O O O O S D U S D O S S S S U U Ammonium Oxalate O O O O O O O O Sd S S Sd S O O O O O O A...

Page 44: ... Butyric Acid S O O O O O O O U Mt U U S O O O O O O Caesium Acetate M O O O O O O O S S S S S O S S O O O Caesium Bromide M O O O O O O O S S S S S O S S O O O Caesium Chloride M O S S O O O O S S S S S S S S S O O Caesium Formiate M O O O O O O O S S S S S O S S O O O Caesium Iodide M O O O O O O O S S S S S O S S O O O Caesium Sulphate M O O O O O O O S S S S S O S S O O O Caesium Trifluoraceta...

Page 45: ...yclohexanone O O O O O O O O Mt U U U U U O O O O O Cyclopentane O O O O O O O O Mt U U U U U O O O O O Decane O O O O O O O O Mt Mt Mt Mt Sd U O O O O O Dextran Sulphate M O O O O O O O S S S S S O S S O O O Diacetone S O U O O O O O S O S S O O S O S O O Diacetone Alcohol S O O O O O O O S O S S O O O O O O O o Dichlorobenzene O O O O O O O O Mt U Mt Mt U O O O O O O p Dichlorobenzene O O O O O ...

Page 46: ... O U U O O O O M U M M O O S M S O O Ethyl Lactate O O O O O O O O O S St S S M O O O O O Ethyl Malonate O O O O O O O O O S Mt S S M O O O O O Fatty Acids S O O O O O O O O Sd Sd Sd S O O O O O O Ferric Chloride U S O O M S S S S O S S O U S S S S S Ferric Nitrate M O O O O O O O S O St S M O O O O O O Ferric Sulphate S O O O O O O O S O S S O O O O O O O Ficoll Paque M O O O O O O O S S S S S O ...

Page 47: ...S U M M Iodine Crystals S O O O O O O O Mt U U Mt U O O O O O O Isobutyl Alcohol O M U O S O O U S S S S O O S S S S S Isopropyl Alcohol O M U U S O S U S M S S M O S S S S S Isopropylbenzene O O O O O O O O Mt U Mt Mt U O O O O O O Kerosene S O O O O O O O Sd S Mt Sd St O O O O O O Lactic Acid 20 O S O O O O S O S S S S S S S S S S S Lactic Acid 100 O S O O O O O O S S S S O S S S S S S Lead Acet...

Page 48: ... O O O S Sd S S St O O O O O O Nitric Acid 50 M U M M U S S M D M M M O D S S S S M Nitric Acid 95 M U U O U O O U M U U M U S S S S S U Nitric Acid conc St O O O O O O O U U Mt U U O O O O O O Nitrobenzene O O O O O O O O U U U U U O O O O O O Octane O O O O O O O O S Sd S S Sd O O O O O O Octyl Alcohol S O O O O O O O O O S O O O O O O O O Oleic Acid S U S S S S O S S S S S S S S S S M S Orange ...

Page 49: ...mCarbonate M O S S O S S S S U S S O S S S S O O Potassium Chlorate M O S S O S S S S S S S O S S O S O O Potassium Chloride U O O O O O O O S S S S S O S S O O O Potassium Hydroxide 5 U M S M U O O S S U S S S S S S M S S Potassium Hydroxide conc U M U U U O O O S U S S O S S S U M U Potassium Nitrate S O O O O O O O S O S S O O O O O O O Potassium Permanganate S O O O O O O O S S S S D O S S O O...

Page 50: ...Sodium Hypochlorite 5 M M S S U S S S D S S S S M S S S S S Sodium Iodide M O O O O O O O S S O S O O O O O O O Sodium Nitrate S O O O O O O O S O Sd S O O O O O O O Sodium Sulfate S O O O O O O O Sd O Sd Sd O O O O O O O Sodium Sulphide S S S O O S O S S U S S O S S S M S S Stearic Acid S O O O O O O O S Sd S S S O O O O O O Sulphuric Acid 10 M U S S U S S S S M S S S U S S S S S Sulphuric Acid 2...

Page 51: ... U U U U O O O O O O Trichloroethane S U S O M S O S U U U U M O S S S S S Triethylamine O O O O O O O O U O O U O O O O O O O Triethylene Glycol O O O O O O O O S Sd S S S O O O O O O Tris Buffer neutral S O O O O O O O S S S S S O S S O O O Trisodium Phosphate O O S O M O O S S O S S O O S S S S O Triton X 10 S O O O O O O O S S S S S O S M O O O Turpentine S O O O O O O O Sd Mt St Sd U O O O O ...

Page 52: ... NOT EXPLO SION PROOF Using this centrifuge with explosive samples is entirely at the user s own risk ELECTRICAL High voltage is present behind the panels of the centrifuge DO NOT remove these panels unless the centrifuge has been first disconnected from the power outlet IMPROPER USE Use only rotors and accessories designed for use in the Jouan MR23i Do not attempt to override the lid interlock as...

Page 53: ...when in a static state it could do so during centrifugation and leak You should there fore follow the instructions of the tube supplier concerning the fill level and sealing of the tube Once a biological risk is known or suspected samples should be pla ced in a sealed container Should such containers not be available sealed accessories should be used such as buckets or angle rotors with sealed lid...

Page 54: ...Chapter 6 HAZARDS PRECAUTIONS AND LIMITATIONS OF USE 6 18 MR23i Thermo Fisher Scientific ...

Page 55: ...o replace the bung in the drain hole in the bowl before the next run Note Never use metallic implements to clean anodised buckets After cleaning the accessories always rinse them with clean water and dry Ideally spray with Thermo Fisher Scientific Anti Corrosion Spray Cat N 11175399 Lubricate the drive shaft and drive shaft O ring with a light oil or silicone spray to prevent sticking PERIODIC PRE...

Page 56: ...ND PREVENTATIVE MAINTENANCE 7 2 MR23i Thermo Fisher Scientific CIRCUIT BREAKER If the main circuit breaker cuts off the power to the instrument do not attempt to switch on before a service engineer has checked over the unit ...

Page 57: ...es defective in normal use or during servicing for a period of ONE YEAR from the delivery Our liability under this guarantee is limited to repairing the defective unit or any part of the unit providing it is sent carriage paid to an authorized service centre or Thermo Fisher Scientific This guarantee is invalidated if the unit is incorrectly used poorly serviced or neglected mis used or accidental...

Page 58: ...Annex A GUARANTEE TERMS A 2 MR23i Thermo Fisher Scientific ...

Page 59: ...ieses Gerätes getroffen Angaben zu Verwertungs und Entsorgungsbetrieben in Deutschland sowie Informationen zu Produkten von Thermo Fisher Scientific die unter die RoHS Richtlinie fallen engl Restriction of the use of cer tain hazardous substances in electrical and electronic equipment Beschränkung der Verwendung bestimmter gefährlicher Stoffe in Elektro und Elektronikgeräten finden Sie im Internet...

Page 60: ...nde per la valorizzazione smaltimento e trattamento in tutti gli stati membri della UE per il riutilizzo o smaltimento e trattamento di questi disposi tivi Indicazioni su aziende per la valorizzazione smaltimento e trat tamento in Germania così come informazioni su prodotti di Thermo Fisher Scientific che rientrano sotto la direttiva RoHS in ingl Restriction of the use of certain hazardous substan...

Page 61: ...om todas as empresas de reciclagem e de elimi nação de resíduos em todos os Estados Membros da UE para que o produto seja reciclado ou eliminado Quaisquer informações sobre as empresas de reciclagem e de eliminação de resíduos na Alema nha bem como sobre produtos da Thermo Fisher Scientific que estão sujeitos à Norma RoHS em inglês Restriction of the use of certain hazardous substances in electric...

Page 62: ...Annex B Declaration of conformity B 4 Heraeus Biofuge Primo R Thermo Fisher Scientific ...

Page 63: ...1 Improper Use 6 16 Inspection 2 4 Introduction 5 3 L Lid Operating 2 2 Lifting and Transport 2 1 M Mains Supply 2 2 Manual Lid Unlocking 2 2 Modify a Program 5 8 O Operational Limitations 6 16 Overwriting Password Protection 5 8 P Password Protection 5 7 Performance 3 1 Periodic Preventative Maintenance 7 1 Positioning 2 2 Precooling 5 4 Preparation 2 4 Preventative Maintenance 6 5 Programming of...

Page 64: ...Index I 2 MR23i Thermo Fisher Scientific ...

Page 65: ......

Page 66: ...ELAND MIDDLE EAST or Tel 7 09 52 25 11 15 Tel 44 87 06 09 92 03 AFRICA Tel 49 61 84 90 69 40 33 2 28 03 20 00 UNITED STATES of AMERICA CANADA or LATIN AMERICA Tel 1 800 522 77 46 Tel 1 800 522 77 46 1 866 984 37 66 1 866 984 37 66 In Other Countries Contact your local Thermo Fisher Scientific products distributor or agent Dans les autres pays Contactez votre concessionaire local des produits Therm...

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