6
– Appendix – Quick Start Guide
Thermo Scientific
Product Manual for Dionex Anion Chemically Regenerated
Suppressor for ICE
Page 22 of 22
032661-12
6.
Appendix – Quick Start Guide
6.1
Important Information
A.
The Dionex ACRS-ICE 500 is installed in the column compartment of the chromatography module right
after the analytical column and before the conductivity detector cell. Refer to Section 2 of the manual for
complete installation instructions.
B.
The operation of the Dionex ACRS-ICE 500 requires a constant flow of the regenerant over the membrane,
in a direction that is countercurrent to the flow of the eluent. A standard regenerant flow rate of 2–3 mL/min
is recommended for most applications.
C.
The recommended regenerant for use with the Anion-ICE MicroMembrane Suppressor (Dionex ACRS-ICE
500) is 5 mM tetrabutylammonium hydroxide (TBAOH).
6.2
Safety
The membranes and screens in the Dionex ACRS-ICE 500 must be completely hydrated to
maintain liquid seals and chromatographic performance. To accomplish this, keep the
regenerant chambers filled with regenerant solution. This will ensure that the membranes
and screens remain properly hydrated.
Back pressures over 450 psi after the Dionex ACRS-ICE 500 can cause irreversible
damage! For optimum operation, the correct amount of back pressure on the suppressor
and cell is 40–50 psi. Be sure to install the correct number of back pressure coils after the
cell, depending on your flow rate.
6.3
Configuration
A.
Connect the eluent line from the ELUENT IN
port of the Dionex ACRS-ICE 500 to the analytical column.
B.
Connect the eluent line from the ELUENT OUT port
of the Dionex ACRS-ICE 500 to the conductivity detector cell.
C.
Connect the regenerant line from the REGEN IN port
of the Dionex ACRS-ICE 500 to the regenerant reservoir.
D.
Connect the waste line from the REGEN OUT port
of the Dionex ACRS-ICE 500 to the waste container.
6.4
Getting Started
A.
Turn on the column eluent flow.
B.
Adjust the reservoir pressure to establish the regenerant flow through the Dionex ACRS-ICE 500.
C.
Pump approximately 5 mL of eluent through the ELUENT IN port and 5 mL of regenerant through the
REGEN IN port of the Dionex ACRS-ICE 500 respectively.
D.
Let the suppressor sit for at least 20 minutes to ensure that the membranes and screens are fully hydrated.
E.
Allow the system to equilibrate before beginning analysis.
CAUTION
!
CAUTION
!
Regenerant
Regen
IN
Regen
OUT
Eluent
OUT
Eluent
IN
Injection
Valve
Guard
Column
Analytical
Column
Waste
Cell
Gas Pressure
25 psi max
Coil #1
Coil #2
