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Thermo Scientific 

Product Manual for Dionex Anion Chemically Regenerated 

Suppressor for ICE  

Page 3 of 22 

032661-12 

© 2015 Thermo Fisher Scientific Inc. All rights reserved. 

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Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product 
operation. This document is copyright protected and any reproduction of the whole or any part of this document is 
strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc. 

The contents of this document are subject to change without notice. All technical information in this document is for 
reference purposes only. System configurations and specifications in this document supersede all previous 
information received by the purchaser. 

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error free and 
assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any 
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Revision History:  

Revision 11, October, 2014, Rebranded for Thermo Scientific. 

Revision 12, March, 2014, Updated backpressure caution statement on page 22 (quick start guide). 

 

 
 
 

 

Summary of Contents for 084714

Page 1: ...Part of Thermo Fisher Scientific Thermo Scientific Dionex ACRS ICE 500 Suppressor Product Manual P N 032661 12 March 2015 ...

Page 2: ...l for Dionex Anion Chemically Regenerated Suppressor for ICE Page 2 of 22 032661 12 Product Manual for Dionex Anion Chemically Regenerated Suppressor for ICE 500 Dionex ACRS ICE 500 9 mm P N 084715 Dionex ACRS ICE 500 4 mm P N 084714 ...

Page 3: ...figurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is...

Page 4: ...a potentially hazardous situation which if not avoided could result in damage to equipment Indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury Also used to identify a situation or practice that may seriously damage the instrument but will not cause injury Indicates information of general interest IMPORTANT Highlights information necessary to prev...

Page 5: ... 1 1 Inorganic Chemicals 14 3 1 2 Solvents 14 3 1 3 Deionized Water 14 3 1 4 Regenerant 14 3 2 Start Up 15 3 3 Determining Eluent and Regenerant Concentrations 16 3 4 Dionex ACRS ICE 500 Storage 16 3 4 1 Routine Use Storage 1 to 5 days 17 3 4 2 Short Term Storage 1 to 3 Weeks 17 3 4 3 Long Term Storage More than 3 Weeks 17 4 Troubleshooting Guide 18 4 1 High Background Conductivity 18 4 2 Drifting...

Page 6: ...ressor devices The suppressor flow pathway has been redesigned to optimize band dispersion and improve the flow and sealing properties The Dionex ACRS ICE 500 continues to use the same cleaned ion exchange components screens and membranes as the Dionex AMMS ICE 300 suppressor device however the Dionex ACRS ICE 500 eluent channel uses an ion exchange resin bed as opposed to a gasketed screen The Di...

Page 7: ...y other counterions of the analytes from the eluent stream and replaces them with tetrabutylammonium ion TBA Since the hydronium ion is the main source of conductance this greatly reduces the background conductivity of the system In addition this mechanism forces the ionization of the weak acid analytes greatly increasing sensitivity The Dionex ACRS ICE 500 is also useful for applications in which...

Page 8: ...ing it with a less conductive cation TBA This process is carried out across the cation exchange membranes Hydronium ions in the eluent cross the membranes and combine with the hydroxide ions in the regenerant to form water At the same time tetrabutylammonium ions cross the membranes into the eluent stream replacing the hydronium ions Waste Analyte A in Eluent Cation Exchange Membrane Cation Exchan...

Page 9: ...ed to temperatures above 50 C for extended periods of time during shipment storage or operation or for short durations above 80 C 1 1 2 Storage Ensure the suppressor is stored in a temperature controlled environment away from direct exposure to sunlight or other sources of heat Do not store the suppressor in an environment where temperatures in excess of 50 C may be experienced such as a parked ca...

Page 10: ... Line Fittings P N 031432 found on the Dionex Reference Library CD ROM 2 2 Eluent Line Connections A Install the Dionex ACRS ICE 500 in the first slot inside the Chromatography Module B Use a short piece of PEEK 0 010 i d tubing with a PEEK 10 32 ferrule bolt fitting on the end of the tubing that will connect to the ELUENT IN port of the Dionex ACRS ICE 500 see Figure 1 On the other end of this el...

Page 11: ...and diameters of tubing necessary for proper back pressure are given in Table 1 Coils for Dionex ACRS ICE 500 Back Pressure Requirements 2 3 1 Assembly A Slip PEEK liquid line bolts and ferrules onto the ends of the tubing Refer to Table 1 Coils for Dionex ACRS ICE 500 Back Pressure Requirements and determine the correct number of coils required for your application based on the eluent flow rate B...

Page 12: ... of this line to the REGEN OUT port of the Dionex ACRS ICE 500 see Figure 1 Direct the other end of the line to waste C Install a restrictor to the regenerant flow by connecting a piece of 0 012 i d tubing to the end of the waste line that is approximately 12 long see Figure 3 D Install the regulator P N 038201 on the helium or nitrogen supply line The regenerant flow rate is regulated by two fact...

Page 13: ...ted Suppressor for ICE Page 13 of 22 032661 12 Figure 3 Configuration of the Pressurized Regenerant Reservoir with the Dionex ACRS ICE 500 Regenerant Regen IN Regen OUT Eluent OUT Eluent IN Injection Valve Guard Column Analytical Column Waste Cell Gas Pressure 25 psi max Coil 1 Coil 2 ...

Page 14: ...e process or improve sample solubility the solvents used must be free of ionic impurities However since most manufacturers of solvents do not test for ionic impurities it is important that the highest grade of solvents available be used Currently several manufacturers are making ultrahigh purity solvents that are compatible for HPLC and spectrophotometric applications These ultrahigh purity solven...

Page 15: ...ic performance To accomplish this keep the regenerant chambers filled with regenerant solution This will ensure that the membranes and screens remain properly hydrated The correct amount of back pressure for optimum operation is 40 50 psi Be sure to install the correct number of back pressure coils depending on your flow rate Back pressures over 450 psi after the Dionex ACRS ICE 500 can cause irre...

Page 16: ...tline Do not use THF solvent in the eluent D 5 mM tetrabutylammonium hydroxide TBAOH is the recommended regenerant for use with the Dionex ACRS ICE 500 Tetramethylammonium hydroxide or potassium hydroxide may be used as alternate regenerants but cause higher background conductivity and therefore compromise total system performance For ease of preparation and guaranteed purity use Dionex Cation Reg...

Page 17: ...ng ports 3 4 2 Short Term Storage 1 to 3 Weeks A Pump 10 mL of 0 2 2 mM Heptafluorobutyric acid or HCl eluent without solvent at 1 0 mL min through the eluent chamber B Fill the regen chambers with TBAOH regenerant C Plug all Dionex ACRS ICE 500 fitting ports D To resume operation connect the suppressor to the system Allow the system to equilibrate before starting analysis 3 4 3 Long Term Storage ...

Page 18: ...aks Also make sure that the regenerant reservoir is pressurized If it is pressurized trace the regenerant lines backward from the reservoir to find and remove any blockage 2 If there is flow from the waste line but it is less than the desired flow rate increase the flow rate by shortening the 0 012 i d waste line tubing or by increasing the reservoir pressure If the flow rate is correct go on to t...

Page 19: ...Regional Office nearest you see Dionex Worldwide Offices 4 2 Drifting Baseline A Increase the regenerant flow rate by decreasing the length of the 0 012 i d restrictor line on the regenerant waste line or by increasing the pressure on the reservoir B Remake the regenerant to be sure that the concentration is correct and that the solution has been freshly prepared Be sure that chemicals of the requ...

Page 20: ...nt and the regenerant through the Dionex ACRS ICE 500 Do not connect the line between the cell and the Dionex ACRS ICE 500 If this inverted operation decreases the back pressure reconnect the Dionex ACRS ICE 500 to the cell and use it in this position E If reversing the flow through the Dionex ACRS ICE 500 does not decrease the pressure clean the membrane see Section 5 F If cleaning the membrane d...

Page 21: ...ntaminants or Precipitates A Disconnect the analytical and guard column s from the injection valve and the Dionex ACRS ICE 500 Refer to the specific analytical column Product Manual for column cleanup procedures B Turn off the regenerant and disconnect the regenerant line from the Dionex ACRS ICE 500 REGEN IN port C Disconnect the liquid line from the Dionex ACRS ICE 500 ELUENT OUT port to the cel...

Page 22: ...res over 450 psi after the Dionex ACRS ICE 500 can cause irreversible damage For optimum operation the correct amount of back pressure on the suppressor and cell is 40 50 psi Be sure to install the correct number of back pressure coils after the cell depending on your flow rate 6 3 Configuration A Connect the eluent line from the ELUENT IN port of the Dionex ACRS ICE 500 to the analytical column B...

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