Age-Related Effects on Hearing
Many lab subjects experience hearing loss due to age. The rate of loss varies across species
and strains. This effect has been observed as early as 12 weeks in mice.
Anesthesia Considerations
Two types of anesthesia protocols are typically used for ABR testing. Each has bene
fi
ts and
limitations. Check your IRB approved guidelines for dosages and protocol.
Xylazine(XYL) + Ketamine to start, Ketamine to Boost
This aesthetic protocol uses an injectable combination of muscle relaxer and sedative.
Typically, the subject is unconscious in a few minutes, begins to wake ~45 minutes and is
completely awake at ~90 minutes after initial sedation. If a subject wakes early or a test runs
longer than ~45 minutes, a booster of ketamine can be used to keep the subject anesthetized.
Be sure to follow your approved IRB protocol.
Iso
fl
urane (ISO)
This aesthetic protocol uses a fast-acting inhalant with a short duration. The subject is
typically fully sedated within 4-5 minutes and wakes very quickly when the gas is removed.
The Subject | 10
