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Pub No.: OM SC4500-1 

 

 

 

 

 

 

 

 

 

 

 

 

October, 2013 

 

 

 

 

 

 

 

 

 

 

 

 

SC4500 

 

 
 

 

 

 

 

Operator’s Manual 

SHUR

Cut

 4500 

Catalog # SC4500 

Fully Automated Rotary Microtome

 

Summary of Contents for SHURCut 4500

Page 1: ...Pub No OM SC4500 1 October 2013 SC4500 Operator s Manual SHURCut 4500 Catalog SC4500 Fully Automated Rotary Microtome ...

Page 2: ......

Page 3: ...mation on TBS products and services please visit the TBS website at www trianglebiomedical com Scope This document contains basic information on the use and operation of a SHURCut 4500 Microtome and assumes you have received basic training on the instrument Please contact your TBS representative for information not provided in this manual Intended Use The SHURCut 4500 Microtome is designed to sect...

Page 4: ...ntrols and Components Display panel Hand wheel locking mechanism Operator s hand wheel Lever for 360 hand wheel brake Blade holder Section waste tray Operator s hand pad Emergency Stop Power Switch Power Connection Socket and Main Foot Switch Hand Pad Interface Connector ...

Page 5: ...ite Requirement 13 Standard Accessory List 13 Installation 14 Blade Holder 14 Waste Tray 14 Operator s Hand Pad 14 Foot Switch Installation 15 Electrical Connection 16 Section 4 Operation 17 Display Panel and Its Features 17 Display Panel 18 Operator s Hand Pad 19 Blade Holder 22 Specimen Clamping System 23 Clamping the Specimen and Blade Installation 24 Choosing Operational Mode 25 Activating Ret...

Page 6: ...6 P a g e G e n e r a l D a t a H e a l t h c a r e I n c Section 6 Troubleshooting 30 ...

Page 7: ...het product dichiariamo sotto nostra unica responsabilità che il prodotto SHURCut 4500 Fully Automated Rotary Microtome to which this declaration relates is in conformity with the following standard s or other normative documents auf das sich diese Erklärung bezieht mit der den folgenden Norm en oder Richtlinie n übereinstimmt auquel se réfère cette déclaration est conforme à la aux norme s ou au ...

Page 8: ...tive s and Standard s set forth in this document Certifications CE TUV EMC Emissions FCC 47 CFR Part 2 Part 15 CISPR PUB 22 USA EMC Immunity EN 55011 2007 Class B EN 61000 3 2 2006 A2 2009 EN 61000 3 3 2008 EN 61326 1 2006 IEC 61000 4 2 2008 IEC 61000 4 3 2010 IEC 61000 4 4 2010 IEC 61000 4 5 2005 IEC 61000 4 6 2008 IEC 61000 4 8 2009 IEC 61000 4 11 2004 EN 61010 1 Third Edition 2001 EN61010 1 201...

Page 9: ... wheel or the cassette clamp Always remove the section waste tray and blade holder before transporting the instrument Check to make sure that the voltage available at your facility complies with the requirements of this unit Connect the unit using the power cable provided It is critical to connect to a grounded socket Do not operate in rooms with explosion hazards Do not tamper with the safety dev...

Page 10: ...pletely before powering up again Turn the unit off and disconnect the power plug before replacing fuses Only use fuses of the same specification and replace them as described in the manual Safety Devices Hand wheel Locking Mechanism There are two hand wheel locking mechanisms Locking mechanism indicated by 1 on the diagram can be engaged by flipping the corresponding switch and rotating the hand w...

Page 11: ...tion where it protects the blade Emergency Stop Function In case the operator needs to stop all the functions of the microtome the emergency stop button 4 can be pressed It turns off all power to the unit To reset the unit after an emergency stop de energize the unit using the ON OFF switch located to the right of the power cord Then rotate the emergency stop button clockwise until it clicks You c...

Page 12: ...ing modes Continuous Single and Manual are available which the operator can easily switch through A foot switch accessory allows for the operator to control the automatic sectioning Technical Data SC4500 Item Description Dimensions Length 21 6 in 55 cm Width 16 5 in 42 cm Height 12 6 in 32 cm Weight 92 6 lbs 42 kg Environment requirements Working temperature 40 F to 50 F 40 C to 10 C Working humid...

Page 13: ... s Slow and 900µm s Quick Cutting Speed 100 420mm s 10 Section 3 Preparation Installation Site Requirement Place the unit on a well supported table Ensure the table is sitting level Ensure that the operating temperature and humidity is according to spec Ensure that there is nothing obstructing the hand wheel operation Standard Accessory List Part Quantity Microtome 1 Blade holder 1 Quick Release C...

Page 14: ...long the track as shown in the picture and then rotate the blade holder locking lever 2 to lock the blade holder Waste Tray Remove the waste tray 3 out from the box and slide it along the track as shown in the picture Operator s Hand Pad Connect the Operator s Hand Pad 4 to the jack in the back of the microtome 5 as described in the picture ...

Page 15: ...15 P a g e G e n e r a l D a t a H e a l t h c a r e I n c 7 6 Foot Switch Installation The foot switch 7 connects at the back of the unit 6 above the hand pad jack ...

Page 16: ...ocket Before changing a fuse make sure to disconnect power from the unit 1 Insert the fuse 6 into the fuse holder 5 as it is showed in the picture and the insert the fuse holder into the socket 4 2 Energize the instrument with the switch at the rear right side The instrument should initialize 3 After the microtome is energized the display field in the control panel and hand pad will illuminate The...

Page 17: ...g modes The following is a diagram that explains the functions of the display panel LCD Display LCD display Current section thickness trimming thickness section counter Num and CLEAR Clear the displayed counters section counter section thickness sum to 0 MODE Selection Choose between sectioning Continuous automatic sectioning mode Indicator Light Power Power on off Error Coarse feed Problem Manual...

Page 18: ...g Mode Indicator Light Using the MODE button the operator can choose between 3 sectioning modes that are then indicated with corresponding LED lights CONT The unit will go into continuous mode This indicates that once the operator presses the RUN button the unit will automatically section until the operator presses the RUN button again to stop SINGLE The unit will go into single sectioning mode Th...

Page 19: ...ng value Display Area Displays sectioning trimming and the retraction value Trim light trimming mode Sect light sectioning mode Ret On light retraction mode Run light Automatically sectioning in Continuous Single Mode Initiates automatic sectioning SECT sectioning mode TRI trimming mode RET retraction mode ON OFF MEMO Memorizes current position the specimen holding arm FEED Recalls memorized posit...

Page 20: ...n this light is illuminated is the speed adjustment of the sectioning Buttons for adjusting Section Trim and Retraction values Press the plus or minus buttons to set the section trim or retraction value Adjusting range 0 5 to 600µm 0 5 to 2µm 0 5µm increments 2 to 10µm 1µm increment 10 to 20µm 2µm increments 20 to 100µm 5µm increments 100 to 600µm 50µm increments Retraction range From 5µm to 100µm...

Page 21: ...ect the retraction amount Push the TRIM or SECT button to exit RUN Button When the instrument is in Single or Continuous mode the RUN button can be pressed to activate automatic sectioning Hold the RUN button for 3 seconds for the instrument to start automatic sectioning After automatic sectioning is running the only buttons that will work are ones that operate the speed of the automatic sectionin...

Page 22: ...mbly sits on the base plate 1 attached to the microtome The X axis slider 3 allows for 10 degrees of adjustment of the blade with respect to the specimen The pictures below explain which component each lever locks The lever on the left operates the blade holder clamp The lever on the right operates the X axis slider clamp The lever on the left operates the Y axis slider clamp 4 2 1 3 ...

Page 23: ...arriers as small as 12mm in length width The clamps 5 or 6 attach to a connector 4 which attaches it to the adjuster 3 To attach a clamp first attach the connector 4 to the adjuster 3 using four screws Then attach either the Quick Release Clamp using two screws or the C Type Clamp using four screws The specimen clamp adjustor allows for the specimen to be swiveled in the horizontal and vertical di...

Page 24: ...ou can now insert the specimen 6 into the clamp 2 Release the handle 5 the specimen will be clamped automatically To remove the specimen repeat the same operation Always clamp the specimen first before installing the blade to avoid injury Installing the blade Before installing the blade in the blade holder clamp make sure that the blade holder base is secured 9 Then choose the angle of the X Axis ...

Page 25: ...hickness The range is between 5µm and 100µm in 5µm increments The values will be displayed on the hand pad Once the desired retraction thickness is set the TRIM or SECT buttons can be pressed to set the retraction value and be able to start sectioning or trimming Activating Trimming Manual Automatic Mode For manual operation set the instrument to the corresponding mode as described in Choosing Ope...

Page 26: ...amount when the arm is at its lowest position to prevent the specimen from being scrapped by the blade on its way up The arm will then advance forward the full retract amount plus the set trim thickness amount Activating Sectioning Manual Automatic Mode For manual operation set the instrument to the corresponding mode as described in Display Panel and Its Features At this stage the operator should...

Page 27: ...ction will be compressed b The harder the specimen the larger the angle will be needed c If the section is not good please try increasing the angle from zero When finished sectioning turn the hand wheel until the specimen is in the upper most position Lock the hand wheel remove the blade and dispose of it safely Memorized Feed Function Memorized feed function allows for the operator to switch betw...

Page 28: ...ons TBS recommends using PARAGard Paraffin Repellent for cleaning SHURCut 4500 Microtome Please contact your TBS representative for more information Cleaning the Clamp Quick Release Cassette Clamp o Position 1 which is often touched when operating and position 2 which is in contact with the specimens need to be cleaned frequently to ensure there is no contamination C Type Specimen Clamp o Position...

Page 29: ...clean The user must clean the instrument regularly according to recommendations of the manual to consistently obtain the best sectioning quality Only authorized and qualified service personnel may access the internal components of the instrument for cleaning and maintenance Makes sure the unit is off and disconnected from the outlet before starting to clean the instrument The blade must be removed...

Page 30: ...older for proper installation or replace the fuses Contact TBS or review the correct voltage requirements shown in the Technical Data table in Section 2 An alarm is activated after powering the instrument on The cable connecting the hand pad to the instrument is not installed properly Cycle the power off and reconnect the hand pad cable to the instrument Producing thick and thin sections Insuffici...

Page 31: ...bed the specimen again Operate the microtome in a temperature controlled environment The section is extremely compressed wrinkled or jammed together Blade edge is dull Specimen block is warming The inclined angle of the blade is too small There is a buildup of paraffin wax on the blade edge The wax is old or damaged Sharpen the blade change the blade or adjust the blade position in horizontal orie...

Page 32: ...ot section on the uneven edge of the blade or change the blade The section splits while cutting or there is a scratch lengthwise The blade has debris on it The specimen is scratched by hard particles Clean the blade Filter the paraffin The section sticks to the blade The blade edge has debris Clean the blade The blade produces scraping sounds when sectioning and the sections are scratched and show...

Page 33: ...33 P a g e G e n e r a l D a t a H e a l t h c a r e I n c ...

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