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NuGenius/

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User manual

USER MANUAL

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Summary of Contents for NuGenius

Page 1: ...NuGenius NuGenius User manual USER MANUAL ...

Page 2: ...t exposure time 17 3 2 CAPTURING AN IMAGE OF A PROTEIN GEL COOMASSIE SILVER STAINED OR OTHER WHITE LIGHT IMAGE COLONY PLATE AUTORAD MICROTITRE PLATE ETC 18 Step 1 Position sample and choose lighting 18 Step 2 Adjust lens 18 Step 3 Set exposure time 18 3 3 CAPTURING AN IMAGE OF AN AGAROSE SAFE DYE GEL 19 Step 1 Position sample and choose lighting 19 Step 2 Adjust lens 19 Step 3 Set exposure time 19...

Page 3: ... PRINTER 27 6 5 DATE TIME 28 CHAPTER SEVEN TROUBLESHOOTING AND CONTACT INFORMATION 29 7 1 TROUBLESHOOTING 29 7 2 CONTACT SYNGENE 29 APPENDIX A LOOKING AFTER YOUR NUGENIUS NUGENIUS SYSTEM 30 APPENDIX B DISPOSING OF YOURIMAGINGSYSTEM 31 ...

Page 4: ...ctly follow the instructions provided Warning notices We use Warnings to highlight information or instructions that MUST be followed to avoid personal injury to yourself or other people in the vicinity For example Switch off the mains voltage and remove the mains cord before cleaning Ensure that all instrument operators read and understand the precautions listed below You are advised to post a cop...

Page 5: ...s symbol is a mandatory marking for devices entering the European market to indicate conformity with the essential health and safety requirements set out in European Directives Symbol for Manufacturer This symbol shall be adjacent to the name and address of the manufacturer Symbol for temperature limitation The upper and lower temperature limits will be indicated on either side of the symbol Symbo...

Page 6: ...he potential hazard and any precautions or other actions that must be taken Warning Labels The following labels are displayed on the outer surfaces of the instrument UV Safety Warning The NuGenius NuGenius imaging system has a UV Transilluminator If you open the door the system will automatically switch the UV off If you wish to override the UV please refer to section 6 3 UV Warning Electrical Saf...

Page 7: ...propriate clothing to protect potential exposure to areas of skin face arms and hands for example N B The door safety interlock is reset when you close the door General operating conditions The NuGenius NuGenius has been designed and tested in accordance with the safety requirements of the International Electrotechnical Commission IEC The NuGenius NuGenius conforms to IEC61010 1 Safety Requirement...

Page 8: ...tributor to advise you about moving your system Environmental conditions The instrument should only be used under the following conditions Indoors Altitudes below 2000m Ambient temperature between 5o C and 40o C Relative humidity below 80 for temperatures up to 31o C decreasing linearly to 50 relative humidity at 40o C Electrical supply fluctuations not exceeding 10 of the nominal voltage The prot...

Page 9: ...y to make the instrument dangerous Intentional interruption of the protective conductor is prohibited Ensure that the electricity supply inlets on the instrument are not obstructed i e leave a gap to allow easy disconnection from the electricity supply When working with the instrument Connect the instrument to a correctly installed line power outlet that has a protective conductor connection earth...

Page 10: ...sation must be expected Electrical Specifications The NuGenius NuGenius power rating is as follows Voltage 100 240Vac Frequency 50 60Hz Current 2A Fuse 2 of 20 mm x 5 mm IEC127 T 6 3 A H 250V Changing fuses There are two sets of fuses that you may need to replace the first set is located near the electrical power cord socket on the rear of the darkroom the second set is located on the rear of the ...

Page 11: ...ance EC directive The NuGenius NuGenius instrument has been designed and tested to meet the requirements of the EC directive 2014 30 EU The NuGenius NuGenius instrument complies with the EMC standard EN61326 EMC standard for electrical equipment for measurement control and laboratory use and EN55011 ISM class B RF emissions FCC rules and regulations This product is classified as a digital device u...

Page 12: ...us Coomassie blue stain Copper stain Zinc stain Silver stain SYBR Gold SYBR Green SYBR Safe UltraSafe blue 1 2 Hardware 1 2 1 Specification NuGenius NuGenius Camera 12 16 bit 12 16 bit CCD 1 2 5 inch 2 3 inch Zoom Motorised zoom 8 48 F1 2 aperture and focus Motorised zoom 11 5 69 F1 4 aperture and focus Display 7 widescreen colour touch screen 7 widescreen colour touch screen Filter UV032 filter a...

Page 13: ...or is opened The Transilluminator can be slid easily in and out of the cabinet 1 2 2 3 LCD Touch Screen A built in 7 inch colour LCD touch screen allows users to preview capture print and save images as well as to select various processing functions without the connection of an external keyboard or mouse TRANSILLUMINATOR OPTIONAL For UV white or blue light UV transilluminator slides in and out of ...

Page 14: ...ained gels Syngene also offers a blue light converter that can be placed on top of the UV Transilluminator for safely imaging gels stained with SYBR Safe Gold and Green GelGreen and UltraSafe blue 1 2 3 2 Printer There are two models of printer available for the NuGenius NuGenius Mitsubishi P95DW Canon Selphy CP1200 The drivers are preinstalled 1 2 3 3 Analysis software The NuGenius NuGenius syste...

Page 15: ...enius NuGenius system 2 2 2 Power on off Connect the mains lead from the NuGenius NuGenius system to the mains power supply and switch on the unit There will be a few seconds delay as the system boots After a few seconds a display will appear To turn the internal computer off press the SHUT DOWN button on screen and then select SHUT DOWN NOW The System will now shut down Once the screen has gone b...

Page 16: ...The content of each image is assessed and those areas that are within the dynamic range of the camera are combined The result is an image with a large dynamic range up to 65536 grey levels that encompasses all the brightness ranges existing within the sample without saturation 2 5 Select Regional Power Frequency Press the SETTINGS button on screen select either 50 or 60Hz depending on the electric...

Page 17: ...t in areas of the sample being saturated You can select to display any saturation on screen by pressing the DISPLAY SATURATION button Saturated areas will appear orange Step 3 Set exposure time The exposure time can either be set manually or automatically Adjusting the exposure time will alter the brightness of the image To manually increase or decrease the exposure time use the following icons Cl...

Page 18: ...hat opening the aperture too far may result in areas of the sample being saturated You can select to display any saturation on screen by pressing the DISPLAY SATURATION button Step 3 Set exposure time The exposure time can either be set manually or automatically Adjusting the exposure time will alter the brightness of the image To manually increase or decrease the exposure time use the following i...

Page 19: ... in areas of the sample being saturated You can select to display any saturation on screen by pressing the DISPLAY SATURATION button Step 3 Set exposure time The exposure time can either be set manually or automatically Adjusting the exposure time will alter the brightness of the image To manually increase or decrease the exposure time use the following icons Click on the or to increase or decreas...

Page 20: ... far may result in areas of the sample being saturated You can select to display any saturation on screen by pressing the DISPLAY SATURATION button Step 3 Set exposure time The exposure time can either be set manually or automatically Adjusting the exposure time will alter the brightness of the image To manually increase or decrease the exposure time use the following icons Click on the or to incr...

Page 21: ...ved Unsaved image Saved Image Images can be saved in the following formats JPEG PNG TIFF NOTE Before removing the USB drive we recommend you press the EJECT USB DRIVE button to ensure no data is lost Eject USB Drive button 4 2 Opening images Insert a USB flash drive into the USB port on the front of the instrument Press on the OPEN SAVED IMAGE button Open Saved Image button NOTE Before removing th...

Page 22: ...ius NuGenius User manual 22 4 3 Printing To print directly from the NuGenius NuGenius system press the PRINT icon If the print button is not available you have not selected your printer See section 6 4 for more details on selecting your printer ...

Page 23: ...black bands on a white background or vice versa This icon is particularly useful when trying to see faint bands The Rotate function will allow you to rotate the image in both 5 degree steps by using the and buttons and also rotate to set positions 0 90 180 270 degrees To apply a sharpening filter to your image move the SMOOTHER SHARPER slider to the right When this filter has been applied band edg...

Page 24: ... the position you want it in The EDIT TEXT button allows the user to edit the text The colour of the text can be changed Select from a selection of colours available from the palette The size of the text can be adjusted Press the to increase the font size and the to decrease the font size To remove any text annotations select the text that you wish to remove and then press the REMOVE button 5 3 Di...

Page 25: ...5 Image Enhancement NuGenius NuGenius User manual 25 Zoom settings can be reset at any time Digitally zoom out of your image ...

Page 26: ...creen with the different setting options will then appear 6 2 Strobe compensation Select your regional power frequency settings choose between 50Hz or 60Hz 6 3 UV safety Select PERMIT OVERRIDE if you wish the user to be able to override the UV Safety protocols To turn the Transilluminator on while the door is open this must be selected ...

Page 27: ... WARNING UV light can be hazardous to your health please refer to the UV Safety instructions 6 4 Printer Click SELECT PRINTER and then select the printer you have attached to the NuGenius NuGenius system and then click OK If no printer is connected and or not detected by the system an error message will appear Connect the printer to the system and then select Ok ...

Page 28: ...r Preferences NuGenius NuGenius User manual 28 6 5 Date Time Select SET INTERNAL CLOCK the current time and date will appear To change the time or date select OK and adjust accordingly then select OK to save ...

Page 29: ...is likely the Transilluminator has failed Contact Syngene NOTE Please take UV precautions Print button is enabled but nothing happens when you select it Check the correct printer has been selected If more than one printer is present the system may be unable to distinguish between them Disconnect one and then continue Unable to save images with USB present Check that the options on the Image storag...

Page 30: ...e cleaning You can clean the outside of the NuGenius NuGenius using a soft lint free cloth moistened if required with a little water Mild detergent may be used if necessary Do not use abrasive or solvent based cleaning materials Always perform a patch test on an inconspicuous area before you clean the entire accessory Avoid spilling any liquid into the body of the NuGenius NuGenius and clean any e...

Page 31: ... disposed of as unsorted municipal waste Any products marked with this symbol must be collected separately and in accordance with the regulatory guidelines in your area The objectives of the WEEE Directive are to preserve protect and improve the quality of the environment protect human health and utilise natural resources prudently and rationally Specific treatment of WEEE is indispensable in orde...

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