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LAMBDA 421 OPERATION MANUAL – REV. 1.00N  (20170623) 

29 

Value 

Command Tx/

-Rx 

Ver

Byte 

Count 

Byte 

Offset 

Dec. Hex.  Binary 

Alt-

key-

pad 

entry 

Ctrl-

char 

ASCII 

def./-

char. 

Description 

 

 

 

  

161 

A1 

1010 

0001 0161

  

Move on rising edge as default 

 

 

 

  

162 

A2 

1010 

0010 0162

  

Move on rising edge, then to Filter 0 
on signal falling as default 

 

 

 

  

163 

A3 

1010 

0011 0163

  

Move on falling edge as default 

 

 

 

  

164 

A4 

1010 

0100 0164

  

Move on falling edge, then to Filter 0 
on signal rising as default 

 Rx 

All 

0   

 

   

Echoed command sequence 

 

 

 

 

2  13 0D 

0000 

1101

 

 <CR>

Completion indicator 

 

 

 

 

 

 

 

 

 

 

 

 

Tx  All 

2 0 

246 

F6 

1111 

0110 0246

 

 

Ring buffer trigger by strobe mode  

 

 

 1 

15 

0F 

0000 

1111 0015

^O  

Get mode state 

Rx  All 

246 

F6 

1111 

0110

   

Echoed command byte 

Get 

Trigger 

Pulse 

Mode for 

Ring 

Buffer 

Status 

 

 

 1 

1-4 

01-04 0000 

0001

0000 0100

 

 

 

Mode state 

 

 

 

 

2  13 0D 

0000 

1101

 

 <CR>

Completion indicator 

 

 

 

 

 

 

 

 

 

 

 

 

Get Ring 

Buffer 

Contents 

Tx  All 

2 0 

245 

F5 

1111 

0101 0245

 

 

Get the contents of the ring buffer  

 Rx 

All 

3-19 0  245 F5 

1111 

0101

 

 

 

Echoed command byte 

 

 

 

 1-16 

0-15 

00-0F 0000 

0000

0000 1111

 

 

 

Filter 0-15 * # of filters 

 

 

 

 

2-17 

240 

F0 

1111 

0000

   

End of filters list marker 

 

 

 

 2-18 

13 0D 

0000 

1101

 

 <CR>

Completion indicator 

 

 

 

 

 

 

 

 

 

 

 

 

Tx  All 

2 0 

244 

F4 

1111 

0100 0244

 

 

Command byte 

 

 

 1 

0-15 

00-0F 0000 

0000

0000 1111

0000

0015

 

 

Specified filter position 

Get 

Specified 

Filter’s 

Galvo A 

DAC Value 

Rx  All 

 

   

 

 

Echoed command sequence 

 

 

 

 2 0 

4095 

0000

FF0F

0000 0000 
0000 0000

1111 1111 
0000 1111

 

 

 

Galvo A DAC value encoded into a 16-
bit “unsigned short” (2 bytes), Little 
Endian 

 

 

 

 4 

13 

0D 

0000 

1101

  

<CR>

Completion indicator 

 

 

 

 

 

 

 

 

 

 

 

 

Tx  All 

4 0 

244 

F4 

1111 

0100 0244

 

 

Command byte 

Get 

Specified 

Filter’s 

Galvo B 

 

 

 1 

128 

143 

80-8F 1000 0000

1000 1111

0128

0143

 

 

Specified filter position 

Summary of Contents for Lambda 421

Page 1: ...28 Web www sutter com Fax 415 883 0572 Email info sutter com Lambda 421 Ultra High Speed Optical Beam Combined 4 Channel Wavelength Switching LED Light Source System With Lambda DG 4 External Control...

Page 2: ...II Copyright 2017 Sutter Instrument Company All Rights Reserved...

Page 3: ......

Page 4: ...2...

Page 5: ...0 Hz line voltage This instrument is designed for use in a laboratory environment that has low electrical noise and mechanical vibration Surge suppression is recommended at all times Fuse Replacement...

Page 6: ...is instrument is not recommended and if undertaken is done so at the users own risk Do not operate if there is any obvious damage to any part of the instrument Avoiding Physical Injury while Powered u...

Page 7: ...1 3 Internal External Control Switch INT EXT 7 3 1 4 Dimness Intensity Control Knob 0 11 7 3 2 Indicators 8 3 2 1 Power Light 8 3 3 Connectors 8 3 3 1 Light Beam Combiner Output 8 3 3 2 STROBE BNC Co...

Page 8: ...s controls and connectors 2 Figure 1 2 Stages of pass through and reflection from each input light source 3 Figure 1 3 Lambda 421 combined output based on the pass through and reflection of each input...

Page 9: ...of the system leaving more in depth technical information towards the end of the chapter The next chapter describes how to install the system followed by one or more chapters that provide operation in...

Page 10: ...urces In the diagram below the optical paths are outlined for each position including the reflections that occur Traditionally combining more than two light sources required the use of a dichroic ladd...

Page 11: ...er losses than previously achievable As an added benefit the last position in the optical train does not require any filter since no other input reflects from that position This input can be used with...

Page 12: ...toward shorter wavelengths While it would be ideal to have a coating optimized for this application we have found that stock STR filters can be used if you correct for the shift in the band pass when...

Page 13: ...ight Guide Liquid Light Guide dovetail with C mount extension Small Zeiss female dovetail USB Interface Cable 9 pin Serial Interface Cable 25 pin Parallel Interface Cable Manual One or more additional...

Page 14: ...6 WARNING DO NOT LOOK DIRECTLY INTO THE LIGHT GUIDE The output of the light or the light guide should be directed into the microscope using the appropriate adapters directed away from anyone s eyes a...

Page 15: ...annel Does not affect externally supplied light source TTL Internally supplied LED light source on off state is controlled by TTL triggering via STROBE 3 1 3 Internal External Control Switch INT EXT T...

Page 16: ...th the SERIAL interface The USB interface is enabled at the factory when the Lambda 421 is shipped unless the SERIAL interface is specifically requested When the USB interface is enabled the SERIAL in...

Page 17: ...o the top position ON turns the on When selecting the lowest position TTL the channel is controlled by the DG 4 interface circuit board The switch to the left is labeled INT EXT In the upper position...

Page 18: ...its that convert the value of the filter selected to a signal that activates a specific LED driver Each LED is gated by a logic level signal and all LEDs will have the analog input connected to the co...

Page 19: ...of any channel if desired The control of the LEDs then is entirely from the DG 4 interface The connections for the DG 4 interface are all available on the back panel Figure 3 2 Rear panel of the Lamb...

Page 20: ...ER VALUE LED SELECTED SUGGESTED CURRENT 0 NONE 0 1 1 100 2 2 100 3 3 100 4 4 100 5 1 50 6 2 50 7 3 50 8 4 50 9 1 33 10 2 33 11 3 33 12 4 33 13 1 15 14 2 15 15 3 15 The actual attenuation is determined...

Page 21: ...ne Maintenance Routine cleaning of the Lambda 421 system is required to prevent excessive dust accumulations Wipe all exterior surfaces with a dry soft cotton cloth Periodically inspect all cables ens...

Page 22: ...LAMBDA 421 OPERATION MANUAL REV 1 00N 20170623 14 This page intentionally blank...

Page 23: ...d to each of the 4 LED channels is adjustable The LED s vary in maximum current from 1A to 5A and adjustment process is straightforward You will the following tools Multimeter 1 5mm Allen wrench Small...

Page 24: ...an LED rated for 5A and switching to an LED rated for 1 or 2A there is a real risk that you can damage the LED accidentally Please follow these instructions carefully 5 2 Adjustment Procedure for Swit...

Page 25: ...5mm allen wrench on lens tube 3 Remove and replace the Filter assembly 4 Turn your Multimeter ON and set to Volts DC 5 Turn on the Main power of the Lambda 421 6 With the LED connected to channel tha...

Page 26: ...LAMBDA 421 OPERATION MANUAL REV 1 00N 20170623 18 FG OBC 561 2 FG OBC 590 2 FG OBC 630 5 FG OBC 660 2 FG OBC 740 1 FG OBC 850 1 FG OBC 940 1...

Page 27: ...id this warranty Warranty work will be performed only at the factory The cost of shipment both ways is paid for by Sutter Instrument during the first three months this warranty is in effect after whic...

Page 28: ...LAMBDA 421 OPERATION MANUAL REV 1 00N 20170623 20 This page intentionally blank...

Page 29: ...wer cord from the power entry module Press down on the lever that is located just above the fuse holder and below the power receptacle and pry the holder straight out of the power entry module Figure...

Page 30: ...LAMBDA 421 OPERATION MANUAL REV 1 00N 20170623 22 This page intentionally blank...

Page 31: ...Dimensions W x D x H 15 75 x 11 x 7 5 in 40 x 27 9 x 19 05 cm Weight 17 8 lbs 8 07 kg Light Guide Core Type Liquid Length 2 m 6 5 ft Diameter 3 mm 0 11 in Electrical Mains voltage 110 240 V 50 60 Hz P...

Page 32: ...67 251 See Note 1 Connected to metal faceplates of connectors on both ends See Note 2 USB USB A USB B 10 ft 3 048 m Note 1 Fair Rite Products Corp P O Box J One Commercial Row Wallkill NY 12589 USA No...

Page 33: ...D 00001101 0013 M 1 Command Move on command to LED 1 at factory defined level 14 0E 00001110 0014 N 2 Command Move on command to LED 2 at factory defined level 15 0F 00001111 0015 O 3 Command Move on...

Page 34: ...and allowing both the before and after commands which have the same value to be accepted as valid commands Execute Next Instance of Previous Command Rx All 2 0 Echoed command sequence 1 13 0D 0000 110...

Page 35: ...0 0000 0000 0000 1111 1111 0000 1111 0000 0000 0255 0015 Filter galvo DAC value encoded into a 16 bit unsigned short 2 bytes Little Endian Set Galvo A or Galvo B DAC Values for All Nominal Filter Posi...

Page 36: ...l 2 0 Echoed command sequence 1 13 0D 0000 1101 CR Completion indicator Tx All 1 0 242 F2 1111 0010 0242 Returns to normal on line mode End Run from Ring Buffer Rx All 2 0 Echoed command sequence 1 13...

Page 37: ...Buffer Status 1 1 4 01 04 0000 0001 0000 0100 Mode state 2 13 0D 0000 1101 CR Completion indicator Get Ring Buffer Contents Tx All 2 0 245 F5 1111 0101 0245 Get the contents of the ring buffer Rx All...

Page 38: ...or Galvo B DAC Values for All Nominal Filter Positions 0 4095 0000 FF0F 0000 0000 0000 0000 1111 1111 0000 1111 0000 0000 0255 0015 Filter galvo DAC value encoded into a 16 bit unsigned short 2 bytes...

Page 39: ...f two bytes ordered in Little Endian least significant byte last format see NOTE 3 The value stored in these two bytes is always unsigned meaning that the value will always be positive negative values...

Page 40: ...LAMBDA 421 OPERATION MANUAL REV 1 00N 20170623 32 This page intentionally blank...

Page 41: ...ctions 5 introduction 2 features 2 general description 2 technical support 1 L LED control switch 7 LED control switch 7 light beam combiner output 8 M mains fuses iii Maintenance 13 N notes user 34 O...

Page 42: ...cations 23 dimensions 23 light guide 23 electrical 23 cables 23 mains fuse 23 mains voltage 23 power cord 23 lamp life 23 output range 23 lamp 23 light guide dimensions 23 weight 23 technical support...

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