6
STEINDORFF
®
VI.
OPERATION:
1.
Illumination:
Turn on the power switch; adjust the brightness adjusting knob slowly to the desired level. In
commonly instance, don’t let it reach the lightest state, otherwise, that shorten the life of the bulb.
2.
Focusing:
Begin observation of your specimen with the 4× Objective. Turn the coarse focusing knob until a
clear image is obtained, then use the fine focusing knob to enhance the observation of the
specimen to its clearest image. When the desired view is obtained under the lowest power (4×),
rotate the nosepiece to the next higher magnification (10×). The nosepiece should “click” into
position. You can obtain clear image with a little fine adjustment.
It is important to remember when adjusting the focus that the objective should never touch the
specimen. When increasing to 100× magnification, the objective will appear to be very close to
the slide. Because the 100× is an oil immersion objective, a drop of cedar-wood oil or other
immersion oil should be applied in the gap between the objective and the specimen. (Note: All
slides should be prepared with cover slips over the specimen for observation under the 100×
objective.) The 100 × oil immersion objectives should be wiped off with a piece of soft clean
cloth or lens tissue to remove the immersion oil immediately after using.
3.
The center of objective superposition with the rotatable mechanical stage:
When the sample is clear enough, find a clear object and let
it coincide with the point of cross. Turning mechanical stage,
if axes of objective don’t coincide with the center of
mechanical stage, the point of object will circumrotate
around “S”. The track is circle, and adjust center of objective
to let “O1” move to “S” to see if they coincide or not. If not,
please adjust them.
4.
Adjusting Abbe condenser:
1)
The Abbe condenser can be moved up or down by
turning condenser focusing knob. When using high power objectives, better to making
the condenser up and whereas making the condenser down. Iris diaphragm should be
adjusted according to objective’s numerical aperture, in order to obtain better effect.
2)
Take off the eyepiece to observe the center of aperture diaphragm on the condenser
overlay with center of objective's aperture. If both is away, may be adjusted the
condenser center adjusting screw until both is superposition.
