Stat Fax 303 Plus, Operator'S Manual

Page: 27 / 52

Awareness Technology, Inc. Stat Fax 303 Plus Operator’s Manual Rev. J 23
2.2 Calculation Programs
Be sure to familiarize yourself with the general selections in section 2.1. Unless otherwise
specified, all general selections are available as described. In addition, all modes may be stored
and recalled in the user test menu (section 2.3), and any additional prompts that appear in
recalled tests are discussed in section 2.3.3. Note that the Absorbance and Uptake Modes do
not have curve storage. When selected, all modes print the date, time, mode, and strip type (8 or
12). The instrument automatically transports the samples through the optical system and back
out again, with the carrier position and readings displayed and printed simultaneously. The
instrument reads and prints results of an 8 well strip in about 30 seconds. Sample printouts from
all modes are found in Section 4.3, Appendix 3.
2.2.1 Absorbance Mode
In this mode, absorbances will be read at the user selected wavelengths, and the absorbance
will be displayed and printed. Use of a blank is optional.
After the filters are selected, the reader displays “Set Carrier” , “Then Press Enter” . Press the
BLANK key to blank on the first well. - “Blank(s) in well 1” is printed. Position the carrier in the
instrument to read your first strip and press ENTER. Absorbance mode does not require the first
strip to be in the right-most carrier position. The instrument reads the strip and simultaneously
displays and prints the results. If blanking was selected, the letter
“B” will appear beside the first
well number
When the instrument is ready for the next strip, the display will indicate “Set Carrier” , “Then
Press Enter” . To continue reading in the Absorbance mode, position the carrier for the next strip
and press the ENTER key. Since there are no concentration interpretations in absorbance mode,
the cutoff and control options are not available.
Press the CLEAR key twice to cancel the mode.
2.2.2 Calibrator (Single Standard) Mode
In this mode, a single calibrator material of known concentration is used to calculate
concentrations of unknown samples according to Beer’s Law. Well #A-1 (the first well of the
right-most carrier position) is always considered to be the blank, with the calibrator well(s)
immediately following. If the duplicate blank option is selected, the first two wells contain the
blank material.
After the filter, duplicates, and unit options, the instrument will display “ Cal 1 = ” . Type the
concentration value assigned to your calibrator material, then press the ENTER key. The printer
will print
“Cal 1 = XXXX” , where XXXX is the calibrator value you have entered. Note that the
instrument allows calibrator values up to 999999, and calibrators less than 1000 can have up to
two places after the decimal point. Decimal places for the reported concentrations will be the
same as those selected for the calibrator.
The display will then prompt “ Cal Limits Y/N” . Select NO to continue, or select YES to enter
absorbance limits for the calibrator, the display will prompt “Cal 1 >=” . Enter the minimum
acceptable absorbance value for the calibrator.
“Set Carrier to X” , “Then Press Enter” is displayed, where X is either 1 or A, depending on the
strip type. Load the first strip into the right-most carrier position and press ENTER. While the
strip is being automatically positioned for reading, the display will indicate
“Positioning Strp” .