Stat Fax 303 Plus Operator'S Manual Download Page 20

16 

Stat Fax 303 Plus Operator’s Manual Rev. J

 

Awareness Technology, Inc. 

13. Will this test be stored? If so, you must remember to store it before you cancel the mode. 

14. For modes using duplicate calibrators, the instrument will ask you if you accept the standard 
 

curve before proceeding. What criteria will be used to determine “bad curves?” Then, you will 

 

be asked which points you wish to delete. Again, some preestablished criteria for selecting 

 

“bad points” and perhaps a maximum number of acceptable deletions might be needed. 

NOTES:  

1.  For the purposes of this manual, the terms “standard(s)” and “calibrator(s)” are used 
 

interchangeably to designate reference materials of known concentrations. The terms 

 

“specimen(s)” and “sample(s)” are also used interchangeably to mean materials of unknown 

 concentrations. 

2.  Pressing the ENTER key will bypass many unwanted options. 

To begin, turn the instrument on and perform the Check Out Procedure (Section 1.4.5). When it 
is finished, the instrument will display 

“Set Carrier”

. Press the CLEAR key twice to cancel the 

Check Out. See 

“Ready”

 and the time in the display. 

If you do not wish to perform the Check Out, turn the instrument on and see 

“Ready”

 and the 

time in the display. The lamp will warm up for 45 seconds. During this time, you may begin 
making your selections. If the lamp warm up is not completed by the time you press the ENTER 
key to read the first strip, the display will indicate 

“LAMP WARM UP 00”

 where 00 represents 

the number of seconds remaining. When the instrument is ready, a double beep will be heard, 
and the instrument will begin reading. 

GENERAL OPERATION TIPS: 

• 

Wipe any dust or fingerprints from the bottoms of the strips before using. 

•   Do not read strips that contain bubbles or condensation. 

•   Use the same volume for the blank as you use for the samples. 

2.1.1 Selecting a Strip Format 

Microstrips are typically either 8 or 12 wells in length. Using the keyboard, this instrument can be 
configured for reading and labeling either type of strip. When using the standard 12-well carrier, 
you may still select either strip format. 

First, press the ALT key. ALT stands for alternate functions. You can use this key to access 
several functions, including changing the strip type. The first display is 

“SAVE TEST Y/N”

Respond by pressing the NO key. Next you will see 

“STRIP TYPE Y/N”

. This time, answer with 

the YES key. 

The display will ask 

“8 WELLS Y/N”

.  If you want to read 8 well strips, answer YES and see 

“8

Well Strips (A-H)”

 printed. If you want to read 12 well strips, press NO and see 

“12 Well Strips 

1-12”

 printed. The display will return to “

Ready”

You may change between reading 8 well strips and 12 well strips via the ALT key whenever the 
display indicates 

“Ready”

If a test is stored in the test menu, the strip type will also be saved. 

Summary of Contents for 303 Plus

Page 1: ...Copyright 2001 Doc 303 Revised 04 2007 Rev J Stat Fax 303 Plus Operator s Manual...

Page 2: ......

Page 3: ...g a Strip Format 16 2 1 2 Selecting a Mode 17 2 1 3 Quitting a Mode 18 2 1 4 Selecting Filters 18 2 1 5 Selecting a Blank 19 2 1 6 Selecting Duplicates 19 2 1 7 Unit Codes 20 2 1 8 Selecting Cutoff In...

Page 4: ...bration and Linearity 36 3 0 Additional Tips and Information 37 3 1 Restoring Electronic Calibration 37 3 2 Operating Precautions 37 3 3 Maintenance 38 3 4 Troubleshooting 39 3 5 References 41 4 0 App...

Page 5: ...ates concentrations and prints results It comes with a built in thermal graphics printer with the capability to plot standard curves Besides providing absorbance readings this instrument also offers p...

Page 6: ...e design with a timed lamp saving feature further assures the reliability of this instrument 1 2 Principles of Operation Light energy from an overhead lamp is focused by a lens directed through an ape...

Page 7: ......

Page 8: ...strument may be impaired Use Proper Power Cord Use only the power cord specified for this product and certified for the country of use Ground the Product This product is grounded through the grounding...

Page 9: ......

Page 10: ...the unit should be turned off Look at the rear panel of the instrument to check that the power switch is in the Off position Power Cord Requirements Use only the power cord specified for this product...

Page 11: ...normally if the power supply is interrupted If this occurs turn the instrument off for a moment When you turn the instrument back on it will resume normal operation but a standard curve which was not...

Page 12: ...cut a new straight edge before trying again Drop the roll of paper into the printer paper well in back spooling up any loose paper Replace the paper cover by pinching its sides together and placing it...

Page 13: ...crostrip reader More details on the operation of each feature are provided in section 2 Operating Procedures The voltage select switch is on the base The power switch and serial connector are on the r...

Page 14: ...ptake Mode Pressing the INTRP key causes the reader to ask for positive and negative cutoff points to be used for automatic sample interpretation Pressing the CNTRL key causes the reader to ask for th...

Page 15: ...total number of wells to read and print The key assignments for the filters are as follows For all models key 1 is 405nm key 2 is 450nm key 3 is 492nm key 4 is 630nm 6 filter UV key 5 is 545nm key 6 i...

Page 16: ...CD 16 characters 5x7 dot matrix Internal Printer Thermal dot matrix 20 characters per line Keyboard 16 key membrane switch 4X4 Calculation Modes Single point calibration Multi point calibration with r...

Page 17: ...supplier Humidity 80 for temperature up to 31 C decreasing linearity to 50 humidity at 40 C Mains supply voltage fluctuations not to exceed 10 of nominal voltage Recommended Environmental Conditions...

Page 18: ...14 Stat Fax 303 Plus Operator s Manual Rev J Awareness Technology Inc Notes...

Page 19: ...o aid the user this instrument may be operated very simply in most modes by bypassing optional features For every test the instrument will require a mode selection and filter choices Thereafter only t...

Page 20: ...lections If the lamp warm up is not completed by the time you press the ENTER key to read the first strip the display will indicate LAMP WARM UP 00 where 00 represents the number of seconds remaining...

Page 21: ...gle point standard curve passing through the point 0 0 A blank is required in the first well or first two wells if duplicate blank is selected A factor equal to the concentration of the calibrator the...

Page 22: ...entration 2 1 3 Quitting a Mode If a test is to be saved in the user test menu this must be done before canceling the mode See section 2 3 1 for instructions on Storing a User Test To quit a mode pres...

Page 23: ...ank and calibrators When duplicates are chosen the mean absorbance reading is used as the reference absorbance for the blank and calibrator You may also choose to read specimens singly or in duplicate...

Page 24: ...utoff When a single upper cutoff is used any concentration which is equal to or greater than the cutoff will be labeled as positive The letters P and N are shown in the right most column of the printo...

Page 25: ...the 8 well mode the first set of wells right most carrier position will be Strip 1 row A through H If you are in the 12 well mode the first set of wells right most carrier position will be Row A stri...

Page 26: ...eak apart wells in the 12 well carrier start from the front of row A and fill all 12 locations then begin at the front of row B etc and continue until done Ensure that the wells are firmly placed in t...

Page 27: ...y for the next strip the display will indicate Set Carrier Then Press Enter To continue reading in the Absorbance mode position the carrier for the next strip and press the ENTER key Since there are n...

Page 28: ...can have any positive or negative numerical value including 0 and 1 Press YES After the filters are selected if you chose no differential the Offset Absorbance questions will follow see section 2 4 5...

Page 29: ...w mNeg C mNeg C Type in each value and press ENTER The display will show Num of PCs Type in the number of Positive Controls and press ENTER The display will show Pos C Pos C Type in each value and pre...

Page 30: ...same order described in the regular cutoff mode NOTE Be sure to read the and signs in each display because blank positive control and negative control parameters are entered in a reverse way when usin...

Page 31: ...e in the number of Cutoff Controls and press ENTER The display will show C O C C O C Type in each value and press ENTER The display will show Num of PCs Type in the number of Positive Controls and pre...

Page 32: ...to access the regression mode Regression Y N will be displayed Answer YES This mode calculates a best fit linear equation based upon the standard points It is designed for use with either linear loga...

Page 33: ...right most carrier position and press ENTER While the strip is being automatically positioned for reading the display will indicate Positioning Strp After the strip or strips containing the standards...

Page 34: ...est 2 calibrator points The calibrators absorbances must be either decreasing or increasing down the strip i e if the first calibrator is the darkest the second must be the next darkest etc and if the...

Page 35: ...e is obtained or the mode is canceled To read and calculate specimens answer NO to the question DELETE WELLS Y N The instrument will be ready for the next strip when it displays Set Carrier Then Press...

Page 36: ...Enter To continue reading in the Uptake mode load the next strip and press the ENTER key Press CLEAR twice to cancel the mode 2 3 User Test Menu This instrument offers the operator 8K bytes of non vol...

Page 37: ...u will see DELETE TEST Y N Answer YES When you see DELETE TEST in the display again type in the number of the test you wish to delete and press the ENTER key The instrument will display DELETE TEST Y...

Page 38: ...e lost when the instrument is turned off or unplugged The time will be displayed whenever the instrument displays Ready The time and date will be printed on all assay reports on either printer The dat...

Page 39: ...ORY IS FULL is displayed if you attempt to store more tests than are available DO ABS SET TEST 212 is printed when the factory set absorbance gain calibration has been lost from non volatile memory In...

Page 40: ...e from your instrument supplier This is because in vertical photometry the fill volume and degree of meniscus determine the pathlength and absorbance is proportional to pathlength For lab test results...

Page 41: ...ires only minimal adjustment from the keyboard to remain calibrated Note that the number entered in test 212 is multiplied by the current absorbance factor set to 1 when reset Thus running test 212 an...

Page 42: ...ive foam This reader is essentially a maintenance free instrument To ensure maximum trouble free operation the instrument need only be kept dry Although the filters are well sealed their life may be d...

Page 43: ...on the internal printer After each well is read the result will remain on the display to permit the user to note the result Press ENTER to advance the strip to the next well Problem The lamp does not...

Page 44: ...oblem The printing is dim or incomplete Solution Use only the specified paper available from your dealer Problem The normal operation of the instrument is suddenly interrupted Solution As with all mic...

Page 45: ...rference filter temperature and humidity recommendations adapted from Melles Griot Optical Guide 3 1985 pp 266 269 2 Other engineering data supplied by Awareness Technology Inc Palm City FL 1987 2006...

Page 46: ...42 Stat Fax 303 Plus Operator s Manual Rev J Awareness Technology Inc Notes...

Page 47: ...rument Appendix 2 is a sheet which may be used to document your stored menu tests Appendix 3 is a set of sample printouts of data from each calculation mode Some modes have been set up to show various...

Page 48: ...d water wait mix and read Using predispensed dyes reduces the degree of pipetting precision required to reconstitute the check set strips Since the strip reader looks through the sample from top to bo...

Page 49: ...Awareness Technology Inc Stat Fax 303 Plus Operator s Manual Rev J 45 4 2 Appendix 2 Test Log Microstrip Reader SN ________ User Test Menu Log Page ___ Test Analyte Stored By Date Notes...

Page 50: ...46 Stat Fax 303 Plus Operator s Manual Rev J Awareness Technology Inc 4 3 Appendix 3 Sample Printouts...

Page 51: ...Awareness Technology Inc Stat Fax 303 Plus Operator s Manual Rev J 47...

Page 52: ...he problem support staff at Awareness Technology is happy to assist you and can be reached in the United States by Phone 772 283 6540 Fax 772 283 8020 e mail support awaretech com Mailing address Awar...

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