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SPI Supplies SPI-DRY, Operation Manual

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SPI Supplies SPI-DRY Operation Manual Download Page 23

 

SPI Supplies 

23 

SPI-DRY Critical Point Dryer 

 

 

 

 

 

 

 

 

 

Buffer wash 

 

 

 

 

 

 

 

Buffer wash 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Figure 6.2 - Process Flow Chart 

 

6.3.1 

Washing 

Specimens must be washed free of mucus, blood, serum or any other contaminant likely to be fixed on 
the surface.  The washing medium must be physiological, i.e. must not cause any changes in shape or 
form of the tissue. 

Glutaraldehyde 

Fixation 

Load into 

CPD Chamber 

At 10

0

 C or less 

Fill Chamber 

With Liquid CO

2 

Osmium 

Post-Fixation 

Purge and Soak 

repeatedly until all 

Solvent Removed 

Heat Chamber 

To above Critical 

Temperature 

Dehydration using 

Alcohol or

 Acetone 

Vent Chamber 

Gradually 

Remove Dried 

Specimens 

Dehydration in 

Absolute Grade & 

Molecular Sieve 

Summary of Contents for SPI-DRY

Page 1: ...Rev 2 2 April 2021 SPI DRYTM Critical Point Dryer Operation Manual SPI Supplies Part 13200JE AB...

Page 2: ...1 800 2424 SPI Toll free from USA Canada 1 610 436 5400 FAX 1 610 436 5755 E mail spi3spi 2spi com For further information regarding any of the other products designed and manufactured by SPI Supplies...

Page 3: ...r use of your unit according to the operation manual should result in trouble free operation Any improper use of the SPI Supplies unit through modifications or unreasonable operating procedures will v...

Page 4: ...n Introduces this manual Section 4 General Description Identifies each of the equipment items and provides an overview of their functions and how they work Section 5 Installation Instructions on how t...

Page 5: ...unctionality Signs 10 2 4 5 Serious Damage to Instruments 10 2 4 6 Hazard to Operator 10 2 5 Risk Analysis 11 2 5 1 Personal Operational Risks 11 2 5 2 Hazardous Materials 11 2 6 Good Working Practice...

Page 6: ...Preparation 23 6 3 1 Washing 24 6 3 2 Fixation 25 6 3 3 Dehydration 25 6 3 4 Substitution 26 6 4 Complete Trial Operation Run 26 Section 7 Maintenance 28 7 1 Chamber Components 29 7 1 1 Viewing Window...

Page 7: ...in this Manual 10 Figure 2 5 Typical Warnings as shown in this Manual 10 Figure 6 1 Phase Diagram 21 Figure 6 2 Process Flow Chart 24 Figure 7 1 Cross section of SPI DRY Critical Point Dryer 28 Figur...

Page 8: ...nstrument or modification of the instrument 2 2 Conformity This instrument is supplied in a form that complies with the protection requirements of the EC Electromagnetic Compatibility Directive 89 336...

Page 9: ...ock Caution refer to accompanying documents Easily touched higher temperature parts Warning risk of electric shock Figure 2 1 Hazard Warning Symbols 2 4 3 Hazard Warning Labels used in Equipment Manua...

Page 10: ...anual This typical caution sign is used where serious damage will be caused by incorrect operation of instrumentation They will follow the same form as functionality warnings but with a triangular war...

Page 11: ...ts before use in the vacuum system It should be noted that isopropanol is a flammable liquid and as such should not be used on hot surfaces In addition it is recommended that protective gloves be worn...

Page 12: ...bend from the knees when lifting heavy objects Wear protective clothing when using liquid nitrogen Affix pressurised gas cylinders firmly to walls or racks Use the correct regulating valves on gas cyl...

Page 13: ...ier costs This covers defects which arise as a result of a failure in design or manufacturing It is a condition of warranty that equipment must be used in accordance with the manufacturers instruction...

Page 14: ...t Dryer Temperature and Pressure gauges High pressure CO2 transfer hose Tubing for water connections Tissue Baskets and Holder Tool Kit Spare Parts Kit Operation Manual 4 1 1 Accessories The following...

Page 15: ...to the water jacket to lower the temperature of the chamber to below 20C Samples that have previously been chemically dehydrated are loaded into the chamber which is then filled with liquid CO2 A ser...

Page 16: ...e ends of the water jacket are sealed with two annular end plates and gaskets Two water connectors are screwed into the vessel wall one at each end to allow for connection of the water supply Both end...

Page 17: ...e preparation is carried out with the samples in the baskets to the point where final substitution is made pure ethanol acetone amyl acetate etc At this point the boat is filled with the substitution...

Page 18: ...ragraph 3 1 5 1 Unpacking Checklist The Equipment package will normally be despatched from the factory in one box Inside the box the following will be found refer and check each item off against the s...

Page 19: ...sure transfer hose to the INLET valve of the pressure vessel The fiber washer is necessary to ensure a good seal Tighten with a wrench Connect the other end of the high pressure transfer hose to the C...

Page 20: ...orphology and ultra structure Since liquid CO2 is not sufficiently miscible with water it is necessary to use an intermediate fluid which is miscible with both water and liquid CO2 In practice interme...

Page 21: ...the TEMPERATURE and PRESSURE gauges As the temperature of the chamber nears 30C the pressure should rise to approximately 1100 psi Once at this point the surface of the liquid will start to dissolve...

Page 22: ...llege London The sequence of operations outlined is very comprehensive In many cases it may be possible to omit some of the stages With difficult specimens however care taken in fixation and dehydrati...

Page 23: ...ing medium must be physiological i e must not cause any changes in shape or form of the tissue Glutaraldehyde Fixation Load into CPD Chamber At 100 C or less Fill Chamber With Liquid CO2 Osmium Post F...

Page 24: ...h routes if any give the best results It may well be that they all give similar quality The most convenient method is route a as it takes the shortest time and uses the cheapest transition fluid Route...

Page 25: ...om the chamber fill the chamber with liquid and close all of the gas control valves Allow 1 hour for the liquid glass to impregnate the samples Please note this time is only a guide for tissue samples...

Page 26: ...door and remove the liquid transfer boat with the now dried samples t Mount the samples on the desired mount and prepare them for examination in the SEM or other instrument NOTE If the system is to b...

Page 27: ...mance it is recommended that the following procedures be carried out on a regular basis a Regularly clean the inside and outside surfaces of the pressure vessel including the O ring and associated sea...

Page 28: ...se b Unscrew and remove the DRAIN valve and adapter plug c Remove the insert and O ring for inspection and cleaning d Re assemble in reverse order using a new O Ring and bonded seal 7 1 4 Gas Control...

Page 29: ...ing is carried out rapidly The use of a thermal transfer grease between the thermometer stem and the pressure vessel will reduce this lag b Pressure Gauge a Using a well fitting open end wrench on the...

Page 30: ...g We hope that you experience the minimum of problems throughout the lifespan of the instrument but inevitably problems may occur Any known problems associated with this type of instrument have been l...

Page 31: ...as they occur To maintain the equipment to the best operating conditions a maintenance schedule is suggested as part of a fault prevention programme the following items are suggested to be included i...

Page 32: ...umbo Safety Valve Bonded Seal EPDM fits both Regular and Jumbo Window O Ring Nitrile fits both Regular and Jumbo Window O Ring EPDM fits both Regular and Jumbo Control Valve Seating O Ring Nitrile Con...

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