Shodex IC SI-36 4D Operation Manual Download Page 1

ver.200603E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

Operation Manual 

Shodex

 IC SI-36 4D 

 

Please read this manual carefully to achieve the best and consistent column performance for a long time

 

 

 

Important Handling Instructions 

 

  Caution! 

 

 

· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column 

and understand their proper handling methods to prevent potential health hazards or death 

from occurring. 

 

·

 

Please wear appropriate personal protective equipment such as lab goggles and gloves 

when handling organic solvents and acid and alkaline reagents. Avoid any direct physical 

contact to prevent chemical injuries.   

 

Before Using the Column 

(1) Please visually inspect the package and outside of the column for any damage.   

 

(2) Please check if product name and serial number written on the column package, adhesive label, and   

enclosed Certificate of Analysis (CoA) are matching and correct.   

 

1. Introduction 

Thank you for purchasing the Shodex

  product. Shodex

  IC SI-36 4D is a

  suppressor type ion 

chromatography column for analysis of anions. The column is used with hydroxide eluents such as 

potassium hydroxide and sodium hydroxide. The column is suitable for the separations of standard anions 

including fluoride, chloride, nitrite, bromide, nitrate, sulfate, and phosphate, as well as sulfite. 

 

 

2. Column Components   

 

 

 

 

 

        

 

 

 

 

 

 

3. Column Specifications 

Product 

Code 

Product Name 

Column Size (mm) 

Particle Size 

m) 

Theoretical Plate 

Number 

(Per Column) 

I.D. 

Length 

F6999361 

IC SI-36 4D 

4.0 

150 

  3.5 

≥ 

8,500 

F6709620 

IC SI-90G 

4.6 

 

10 

(Guard Column) 

 

Base Material 

: Spherical porous particles of polyvinyl alcohol modified with quaternary ammonium group

 

Column Housing : PEEK   
Screw Type 

: Internally-threaded type No.10-32 UNF 

Shipping Solvent : 10 mM Sodium Sulfate Solution (SI-36 4D) 
 

: 1.8 mM Sodium Carbonate So 1.7 mM Sodium Bicarbonate Solution (SI-90G) 

 

 

 

Summary of Contents for IC SI-36 4D

Page 1: ...ckage adhesive label and enclosed Certificate of Analysis CoA are matching and correct 1 Introduction Thank you for purchasing the Shodex product Shodex IC SI 36 4D is a suppressor type ion chromatography column for analysis of anions The column is used with hydroxide eluents such as potassium hydroxide and sodium hydroxide The column is suitable for the separations of standard anions including fl...

Page 2: ...e is less than 10 25 μL per column When analyzing low concentration samples 0 1 mg L increasing the injection volume to 100 200 μL may improve the detection 4 For the samples containing proteins remove the proteins prior to the sample injection The use of an ultrafiltration membrane is recommended 5 For the samples containing hydrophobic impurities remove the impurities prior to the sample injecti...

Page 3: ...ss than 0 35 mL min at the end of analysis Then turn off the column oven and let the column temperature return to room temperature before stopping the pump If the pump was stopped while the eluent inside the column was still hot as the eluent temperature decreases its volume also decreases This may result in creating an empty space in the column and deteriorates the column 6 3 Eluents 1 Use freshl...

Page 4: ...cleaning steps for adsorbing components For an efficient cleaning reverse the flow direction and use the flow rate at 0 35 mL min Method 1 Adsorption of hydrophilic compounds Washing Solution Cleaning Time 1 H2O 25 min 2 100 mM KOH or NaOH aq solution 100 min 3 H2O 25 min 4 Eluent 100 min Method 2 Adsorption of hydrophobic compounds Washing Solution Cleaning Time 1 H2O 25 min 2 H2O CH3CN 95 5 20 m...

Page 5: ...late Number N 2 Asymmetry Factor Fas 9 Additional Warnings 1 Do not remove end fittings 2 Do not make a strong impact on the column Do not drop or hit the column on a hard surface Refer to the Shodex website https www shodex com for product details and their applications For additional assistance contact the distributor from whom you purchased the column or contact your regional Shodex support off...

Page 6: ... anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained...

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