46 |
P a g e
MAINTENANCE
C
ALIBRATE THE
T
EMPERATURE
D
ISPLAY
Note:
This procedure requires a temperature reference device. Please see the
on page 10 for the device requirements.
Temperature calibrations match the temperature display to the actual air temperature inside the
incubation chamber. The actual air temperature is supplied by a reference sensor device. Calibrations
compensate for software drifts in the controller as well as deviations caused by the natural material
evolution of the sensor probe in the heated chamber space. Calibrate as often as required by your
laboratory or production protocol, or regulatory compliance schedule. Always calibrate to the industry
or regulatory standards required for your application.
Humidity
Humidity affects temperature uniformity and stability in the incubation chamber. The chamber must
be humidified for at least 24 hours in order to conduct an accurate calibration.
A Suggested Calibration Set Up
CO
2
Calibration
: A CO
2
calibration may be
performed during the temperature calibration so
long as the chamber door and access port
remain closed and sealed.
1.
Introduce the reference device
thermocouple sensor probe through the
access port on the right side of the incubator.
2
. Position the probe in the chamber.
•
Place the probe head as close as
possible to the geometric center
point of the chamber.
•
The probe head must be at least 2
inches (51 mm) from the surface of the
shelving to prevent heatsinking.
3.
Secure the probe head in position with
the non-marking, heat-resistant tape.
4.
Carefully place the access port stopper in
the port over the probe wires. Use non-stick
tape to seal any gaps created between the
stopper and the port by the probe wires.
5.
The incubator door must be closed and
latched. Failure to do so will prevent an
accurate calibration.
2” (51 mm)
Use non-marking, heat-resistant polyamide tape
to hold the thermocouple probe in place. The
manufacturer recommends Kapton brand tape,
0.5 inches width (12.7 mm), 2 mil thickness.