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Introduction
EasyPREP
BOD-300
is an automated offline system for the sample preparation and analysis for Biochemical Oxygen Demand (BOD).
With three liquid-handling peristaltic pumps, the
EasyPREP
BOD-300
adds seed and nitrification inhibitor as well as dilution water to
perform sample dilutions. The low maintenance dissolved oxygen (DO) probe with either optical or membrane technology incorporates a blade
stirrer, keeping the sample homogeneous and accelerating probe equilibration for DO measurement. The software manages sample's initial to
final DO measurement and BOD calculations. User-definable QC control allows flagging and validation of analytical batches. Preparation and
results conform to established methods ISO 5815-1/EN-1899-1/2./5814 (2012), EPA 405.1 and Standard Methods 5210B.
The
EasyPREP BOD-300
accommodates 60 ml, 165 ml or 300 ml bottles for optimized throughput, allowing the analysis of up to 60
position (300 ml bottles), 72 position (60 ml bottles) or 72 position (165 ml bottles) without operator intervention.
Biochemical Oxygen Demand (BOD) analysis is an empirical bioassay-type procedure which determines the dissolved oxygen consumed
by microbial life while assimilating and oxidizing the organic matter present in the sample water. The standard test conditions include dark
incubation at 20 °C for a specific time period (often 5 days, or 7 days), in an entirely filled and closed bottle. Dissolved oxygen (DO) is
measured before and after incubation, and the BOD is computed from the difference.
Carbonaceous biochemical oxygen demand (cBOD) method differs from the BOD method. cBOD includes nitrification inhibitor added in the
bottles on Day 0. Nitrification inhibitors available in the market include: allylthiourea (ATU) or 2-chloro-6-(trichloromethyl) pyridine (TCMP). The
nitrification inhibitor is added by its own peristaltc pump in liquid form.
