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Instruction manual 

 

 

 

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Summary of Contents for MCO-5M

Page 1: ...from Instruction Manual x If you find out Refer to page ƔƔ in them this page means not page in service manual but page in the lower corner of each page in the extract from Instruction manual This page number is not corresponded with serial number in Service manual ...

Page 2: ...56 MCO 5M Multi gas Incubator INSTRUCTION MANUAL ...

Page 3: ...erilizing of chamber and attachments P 18 Connection of N2 or O2 gas cylinder P 19 Connection of gas injection nozzle P 20 Connection of CO2 gas cylinder P 21 OPERATING INSTRUCTIONS Set of chamber temperature and CO2 density P 22 UV lamp P 23 Change of setting of UV lamp ON period P 24 Automatic gas cylinder changeover P 25 Key lock function P 27 Water level sensor P 28 ALARMS SAFETY FUNCTIONS P 2...

Page 4: ...ion is prohibited this symbol means an instruction must be followed Be sure to keep this manual in a place accessible to users of this unit Label on the unit This mark is labeled on the cover in which the electrical components of high voltage are enclosed to prevent the electric shock The cover should be removed by a qualified engineer or a service personnel only WARNING As with any equipment that...

Page 5: ...ground the unit through a gas pipe water main telephone line or lightning rod Such grounding may cause electric shock in the case of an incomplete circuit Check the gas type and ensure that it is fit for the purpose Make sure that all pipes are connected correctly and are not liable to become disconnected Ensure that the gas pressure is set at the specified value Improper connection of the gas pip...

Page 6: ...y Never expose the eyes directly to UV light as UV light can cause permanent damage to eyes Never remove cover when UV light is ON when an optional UV system kit MCO 18UVS2 is installed Hazardous UV light Do not press door switch Never disassemble repair or modify the unit yourself Any such work carried out by an unauthorized person may result in fire or injury due to a malfunction Disconnect the ...

Page 7: ...l Current leakage or electric shock may result form deterioration of insulation by spilled water Do not climb onto the unit and do not put articles on the unit This may cause injury by tipping or damage to the unit When stacking the unit follow the procedure shown on page 35 Always hold the handle when closing the door This will reduce the likelihood of a trapped finger Do not damage the power sup...

Page 8: ...ed for long periods The deteriorated insulation may cause electric shock current leakage or fire Do not put the packing plastic bag within reach of children as suffocation may result Take care of the inside of the outer door It may get hot Use the power supply cord enclosed with the unit Use of an inferior power supply cord may cause electric shock or fire CAUTION ...

Page 9: ...in the direct air flow from an air conditioning system Cool air from an air conditioning system may cause condensation and lead to possible contamination 5 Always keep the chamber clean The condensation may be caused on the inside of the door by spilled water form humidifying pan or opening of outer door for long period Wipe off the condensation completely with a sterile dry gauze Especially when ...

Page 10: ...dition immediately when the alarm is activated Refer to page 25 for alarm details 13 Do not use CAL key Do not use CAL key on the control panel in normal use Pressing this key leads the calibration mode Wrong key operation affects the basic performance Never touch any other keys on the control panel in the event of pressing CAL key accidentally After about 90 seconds the unit returns to chamber te...

Page 11: ...r in which the electrical components of high voltage are enclosed to prevent the electric shock The cover should be removed by a qualified engineer or a service personnel only This symbol means UV caution This symbol means attention or refer to document This symbol means hot surface This symbol means earth This symbol means power switch ON This symbol means power switch OFF ...

Page 12: ...o notify the recommendation of UV lamp replacement when the accumulated ON time of UV lamp is over about 1 000 hours The blink of the UV indicator on the control panel recommends the replacement of UV lamp For the replacement contact Sanyo sales representative or agent E18 will be displayed on the temperature indicator when the UV lamp is burned out Contact Sanyo sales representative or agent for ...

Page 13: ...ft side Rear right side 10 20 1 2 3 4 5 12 6 7 inside 8 13 15 16 inside 19 14 When MCO 18UVS2 is installed When MCO 18UVS2 is installed 18 Switch cover 19 8 9 17 22 21 Chageable Connecting port for N2 O2 gas Connecting port for CO2 gas 11 23 ...

Page 14: ...05MPaG 0 5kgf cm2G 7 1psiG Refer to page25 for automatic cylinder changeover 12 Door switch Detects the door opening closing and stops the circulating fan and electromagnetic valve for CO2 and N2 O2 when door is open UV lamp is also deactivated by door opening When an optional UV system kit MCO 18UVS2 is installed 13 Humidifying pan Use sterile distilled water to fill the pan 14 Humidifying pan co...

Page 15: ...regulator is used to set the upper temperature limit 7 Over heat lamp OVER HEAT This lamp lights when the chamber temperature reaches the upper limit set value 8 CO2 inject lamp INJECT This lamp lights when CO2 gas is being injected 9 Digital CO2 density indicator CO2 Normally this indicator shows the CO2 concentration in the chamber In the setting mode it indicates the set value of the CO2 concen...

Page 16: ...der B 17 Gas supply line switching key GAS SUPPLY This is a key to select N2 or O2 gas supply By pressing this key the N2 or O2 gas supply line A B is changed in O2 density set mode The supply line is changed automatically when N2 or O2 gas cylinder is empty Similarly by pressing this key in CO2 density set mode CO2 gas supply line is changed automatically when an automatic gas cylinder changeover...

Page 17: ...V 2A Note When the power switch is OFF or the power failure condition the contact output is CLOSE The remote alarm cannot be silenced by pressing the alarm buzzer stop key BUZZER since the remote alarm is not conjunct with the BUZZER key The contact output is kept even though the remote alarm is silenced by pressing buzzer stop key BUZZER Remote alarm terminal Remote alarm terminal REMOTE ALARM MA...

Page 18: ...here the incubator is installed 3 A location away from heat generating sources 4 A location with a sturdy and level floor WARNING Install the unit on a sturdy floor If the floor is not strong enough or the installation site is not adequate this may result in injury from the unit falling or tipping over Select a level and sturdy floor for installation This precaution will prevent the unit from tipp...

Page 19: ... packaging materials and tapes Remove all transportation packaging materials and tapes Open the doors and ventilate the unit If the outside panels are dirty clean them with a diluted neutral dishwashing detergent Undiluted detergent can damage the plastic components For the dilution refer to the instruction of the detergent After the cleaning with the diluted detergent always wipe it off with a we...

Page 20: ...with a diluted neutral dishwashing detergent and then wash out the detergent with distilled water Undiluted detergent can damage the plastic components For the dilution refer to the instruction of the detergent 3 Wipe the attachments with a gauze containing alcohol for sterilization and then wipe off with a dry gauze 4 Wipe the inside wall of the chamber with a gauze containing alcohol for sterili...

Page 21: ...or O2 inlet A B located at the rear left side of the incubator Connect the main cylinder to the inlet A and connect the sub cylinder to the inlet B so that the gas supply can be switched automatically when one gas cylinder is empty Connect the same gas type to both inlet A and B Never connect N2 gas and O2 gas at the same time to the inlet A and B respectively 3 Set the N2 or O2 gas pressure on th...

Page 22: ...midity after opening of the inner door Fill the humidifying pan with sterile distilled water so that the injection nozzle can be under the water level 1 Inside of unit is arranged as shown in Fig 1 when humidifying pan cover removed There is a gas injection port in the lower left hand corner 2 Connect gas injection nozzle to gas injection port using connecting pipe Fig 2 3 Connecting gas injection...

Page 23: ...kgf cm2 G 30psiG on the secondary side 2 The connecting port for CO2 gas is located at the rear left side of the incubator see page 11 Use the port A only when MCO 5GC is not installed With MCO 5GC installed use the port A and B When MCO 5GC is not installed Using the gas supply pipe provided connect the pressure regulator to the CO2 connecting port A located at the rear left hand side of the incu...

Page 24: ...erical value shift key set the figure to 05 0 When pressed the figure of settable digit changes 6 Press ENT key ENT Set CO2 density is memorized and Left digit in O2 density indicator is flashed When pressed the changeable digit is shifted 7 By pressing digit shift key and numerical value shift key set the figure to 05 0 When pressed the figure of settable digit changes 8 Press ENT key ENT Set O2 ...

Page 25: ...ible blue light can be checked under the humidifying pan cover The UV light is harmful to eyes Never turn on the UV light with the inner door or humidifying pan cover opened The UV lamp is ON for a predetermined period after the outer door is closed or every 12 hours when the outer door is not opened more than 12 hours continuously The period of factory setting is 5 minutes The period can be chang...

Page 26: ...sed the figure of settable digit changes 5 Press ENT key ENT Set value is memorized and the display return to normal display mode The available set range for the UV lamp is between 0 and 30 minutes 000 to 030 When set to 000 the UV lamp is not turned on The UV lamp is turned off during ON period when the outer door is opened After closing the outer door the lamp turns on during predetermined perio...

Page 27: ...dicator O2 density indicator When N2 or O2 gas supply line is switches automatically Current chamber temperature and E02 is displayed alternately Current CO2 density is displayed Current O2 density and empty cylinder is displayed alternately A or b When CO2 gas supply line is switches automatically only when MCO 5GC is installed Current chamber temperature and E01 is displayed alternately Current ...

Page 28: ...he blinking CO2 gas supply lamp changes to continuous ON and cylinder A is selected 4 Press ENT key ENT The setting of CO2 gas cylinder is memorized and the left digit on the O2 density indicator blinks 5 Press gas supply line switching key once 䋨Pressing the key again selects N2 gas cylinder A䋩 The lamp ON of N2 O2 gas supply lamp changes to blinking and cylinder B is selected 6 Press ENT key ENT...

Page 29: ...cedure for key lock setting change from key lock OFF to key lock ON Description of operation Key operated Indication after operation 1 The current chamber temperature is displayed 2 Press digit shift key for 5 seconds L0 is displayed in the temperature indicator 3 Press numerical value shift key and scroll the figure to 1 When pressed the figure of settable digit changes 4 Press ENT key ENT The ke...

Page 30: ...the humidifying pan if the sensor is in lower position after maintenance At the time of installation of humidifying pan check that the pan is set properly and sensor is down in the pan The water level alarm lamp RH PAN blinks if the sensor is not down completely In this case set the pan again in adequate location Use an alcohol for sterilization when cleaning the sensor taking care not to stress t...

Page 31: ...emperature indicator Intermittent tone Remote alarm N2 O2 gas cylinder empty If the O2 density does not change when the N2 O2 gas valve is opened E02 is displayed alternately with the temperature on the temperature indicator Intermittent tone Remote alarm Automatic changeover of N2 O2 gas connection port CO2 gas line changeover When the gas supply line is switched only when MCO 5GC is installed E0...

Page 32: ...rature indicator Intermittent tone Remote alarm Door heater abnormality If the door heater goes open circuit or the door heater relay short circuit E14 is displayed alternately with the temperature on the temperature indicator Intermittent tone Remote alarm Disconnection of S S R for each heater If the relay of main heater bottom heater or sensor box heater goes open circuit E16 is displayed alter...

Page 33: ...s CAL key for 5 seconds The left digit is flashed The settable digit is shifted 3 Set the figure to F25 with the digit shift key and numerical value shift key When pressed the figure of settable digit changes 4 Press ENT key ENT The current setting is displayed The middle digit is flashed 5 Set the figure to 020 with the numerical value shift key When pressed the figure of settable digit changes 6...

Page 34: ...ments Do not clean the inside of the unit with a solution of disodium chlorate or other halogen based solution because this may cause rust 1 Take out all trays and the humidifying pan from the chamber 2 Remove ducts side support right and left side and the fan as shown in the figures on page 33 and 34 3 Clean all the attachments with a diluted neutral dishwashing detergent and then rinse away the ...

Page 35: ...nder and turn off the power 2 Open the outer and inner door 3 Pull out the all trays See Fig 1 4 By lifting up the humidifying pan cover unhook it at the rear and remove the cover as shown in Fig 2 5 Take out the humidifying pan See Fig 3 6 Loosen and remove the screw fixing the clamp and remove the clamp See Fig 4 and Fig 5 7 Remove all tray supports on the right and left side by lifting up the f...

Page 36: ...ttachments in the chamber with the reversed order mentioned above Note Always insert the fan on the motor shaft surely Check that the fan does not hit the rear panel by turning the fan manually Improper insertion may cause poor performance As shown in the figure set the shelf with the edge bent upward Improper setting may cause a tilted or unstable condition Fig 7 Fig 8 Upward bend ...

Page 37: ...water sufficiently Finally wipe the pan with a soft cloth and alcohol for disinfection 4 Wipe off the bottom of the chamber completely 5 Place the pan under the pan cover and pour the sterile distilled water about 1 5 liters into it See Fig 3 Make sure that the water is pre heated at 37o C 6 Push the pan into its correct position and replace the cover over the pan Close the inner and outer door 7 ...

Page 38: ...or goes out 3 Adjust the set value to the actual value of 36 8o C with the key and key then press ENT key 4 The unit automatically reverts to the display mode Note It is important to accurately measure the temperature inside the unit when performing temperature calibration Particularly the temperature gauge used must have an accuracy of 0 5 Class or better The temperature must be measured at sever...

Page 39: ...2 density 1 Press the CAL key for about 5 seconds 2 The third digit on the temperature indicator flashes and the CO2 density indicator goes out 3 Press the CAL key once again 4 The third digit on the CO2 density indicator flashes and the temperature indicator goes out 5 Set the present correct CO2 density with the key and key then press the ENT key 6 The unit automatically reverts to the display m...

Page 40: ...y 1 Press the CAL key for about 5 seconds 2 The third digit of the temperature indicator flashes and the other indicators go out 3 Press the CAL key once again 4 The third digit of the CO2 indicator flashes and the other indicators go out 5 Press the CAL key once again 6 The third digit of the O2 indicator flashes and the other indicators go out 7 Set the present correct O2 density with the key an...

Page 41: ...rimary pressure of less than 3 8MPaG 38kgf cm2 G means a little gas in the cylinder Replace the cylinder soon If the chamber temperature is not equal to the set temperature The ambient temperature must always be at least 5o C less than the set temperature The outer door was closed while the inner door was left open If the gas density does not coincide with the set value The secondary pressure is n...

Page 42: ...oltage 6 Other supply voltage fluctuations as stated by the manufacturer 7 Transient overvoltages according to Installation Categories Overvoltage Categories II For mains supply the minimum and normal category is II 8 Pollution degree 2 in accordance with IEC 664 DISPOSAL OF UNIT WARNING If the unit is to be stored unused in an unsupervised area for an extended period ensure that children do not h...

Page 43: ...t to avoid scratches or damage Fig A 5 Fix the stacking plate A at 2 locations on the top front of the lower unit by using 2 screws contained in accessories bag 6 Remove the front panel on the upper unit by unscrewing the 4 fixing screws and then disconnect the wires and gas tube 7 Stack the unit so that both units can be aligned straight Also check the upper unit is level If it is not level keep ...

Page 44: ...98 42 STACKED MODULE Front panel Stacking plate A Protective sticker Stacking plate B Fig A View from quarter rear side ...

Page 45: ...m Zirconia sensor direct detection in chamber O2 density display Digital display Air circulation Fan assisted Air filter 0 3μm Efficiency 99 97 or more Water level sensor Optical type Alarm High Low temperature alarm CO2 density alarm O2 density alarm Door alarm Upper limit temperature alarm Remote alarm contact Allowable contact capacity DC 30 V 2 A CO2 inlet connection 4 to 6 mm diameter tube CO...

Page 46: ...ture 25o C setting 37o C 5 no load O2 control range 1 to 18 22 to 80 O2 variation 0 2 ambient temperature 25o C setting 37o C 5 no load Chamber humidity 95 5 R H Maximum heat emission 740 kJ h Noise level 24 db A scale Usable environment condition Temperature 5o C to 35o C Humidity equal or less than 80 R H The designed performance may not be obtained when the ambient temperature is equal or less ...

Page 47: ... the unit Unit interior No contamination Decontaminated Contaminated Others 3 Instructions for safe repair maintenance of the unit a The unit is safe to work on b There is some danger see below Procedure to be adhered to in order to reduce safety risk indicated in b below Date Signature Address Division Telephone Product name Multi gas incubator Model MCO 5M Serial number Date of Installation Plea...

Page 48: ...JK1284 250B ...

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